:23S rRNA pseudouridine2457 synthase

{{Short description|Class of enzymes}}

{{Infobox enzyme

| Name = 23S rRNA pseudouridine²⁴⁵⁷ synthase

| EC_number = 5.4.99.20

| CAS_number =

| GO_code =

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23S rRNA pseudouridine²⁴⁵⁷ synthase ({{EnzExplorer|5.4.99.20}}, RluE, YmfC) is an enzyme with systematic name 23S rRNA-uridine²⁴⁵⁷ uracil mutase.{{cite journal | vauthors = Del Campo M, Kaya Y, Ofengand J | title = Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli | journal = RNA | volume = 7 | issue = 11 | pages = 1603–15 | date = November 2001 | pmid = 11720289 | pmc = 1370202 }}{{cite journal | vauthors = Pan H, Ho JD, Stroud RM, Finer-Moore J | title = The crystal structure of E. coli rRNA pseudouridine synthase RluE | journal = Journal of Molecular Biology | volume = 367 | issue = 5 | pages = 1459–70 | date = April 2007 | pmid = 17320904 | pmc = 1876706 | doi = 10.1016/j.jmb.2007.01.084 }} This enzyme catalyses the following chemical reaction

: 23S rRNA uridine²⁴⁵⁷ $\backslash rightleftharpoons$ 23S rRNA pseudouridine²⁴⁵⁷

The enzyme modifies uridine²⁴⁵⁷ in a stem of 23S RNA in Escherichia coli.