:4'-methoxyisoflavone 2'-hydroxylase

{{Short description|Class of enzymes}}

{{infobox enzyme

| Name = isoflavone 2'-hydroxylase

| EC_number = 1.14.14.89

| CAS_number = 110183-49-8

| GO_code = 0047957

| image =

| width =

| caption =

}}

In enzymology, a 4'-methoxyisoflavone 2'-hydroxylase ({{EC number|1.14.14.89}}, Formerly {{EC number|1.14.13.53}}) is an enzyme that catalyzes the chemical reaction

:formononetin + NADPH + H⁺ + O₂ $\backslash rightleftharpoons$ 2'-hydroxyformononetin + NADP⁺ + H₂O

The 4 substrates of this enzyme are formononetin, NADPH, H⁺, and O₂, whereas its 3 products are 2'-hydroxyformononetin, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is formononetin,NADPH:oxygen oxidoreductase (2'-hydroxylating). Other names in common use include isoflavone 2'-monooxygenase (ambiguous), and isoflavone 2'-hydroxylase (ambiguous). This enzyme participates in isoflavonoid biosynthesis. It employs one cofactor, heme.