:ACES (buffer)

{{chembox

| Verifiedfields = changed

| Watchedfields = changed

| verifiedrevid = 477235210

| ImageFile=ACES_buffer.svg

| ImageSize=

| Name = ACES

| PIN=2-[(2-Amino-2-oxoethyl)amino]ethane-1-sulfonic acid

| OtherNames=N-(2-Acetamido)-2-aminoethanesulfonic acid

|Section1={{Chembox Identifiers

| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}

| ChemSpiderID = 73843

| InChI = 1/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10)

| InChIKey = DBXNUXBLKRLWFA-UHFFFAOYAH

| SMILES1 = O=S(=O)(O)CCNCC(=O)N

| StdInChI_Ref = {{stdinchicite|correct|chemspider}}

| StdInChI = 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10)

| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}

| StdInChIKey = DBXNUXBLKRLWFA-UHFFFAOYSA-N

| CASNo_Ref = {{cascite|correct|CAS}}

| CASNo=7365-82-4

| UNII_Ref = {{fdacite|correct|FDA}}

| UNII = I5SPL5YVBY

| PubChem=81832

| ChEBI_Ref = {{ebicite|correct|EBI}}

| ChEBI = 39062

| SMILES=C(CS(=O)(=O)O)NCC(=O)N

}}

|Section2={{Chembox Properties

| Formula =

| C=4 | H=10 | N=2 | O=4 | S=1

| MolarMass=182.199

| Appearance=

| Density=

| MeltingPt=

| BoilingPt=

| Solubility=

}}

|Section3={{Chembox Hazards

| MainHazards=

| FlashPt=

| AutoignitionPt =

}}

}}

ACES (N-(2-acetamido)-2-aminoethanesulfonic acid) is a chemical compound that is one of Good's buffers. It was developed in the 1960s to provide buffer solutions with pH ranging from 6.15-8.35 for use in various applications. With a pKa of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.Good, N.E., "Hydrogen ion buffers for biological research." "Biochemistry", 5(2), 467–477.

Applications

ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.Liu, Q., et al., "pK-matched running buffers for gel electrophoresis." "Anal. Biochemistry.", 270(1):112-122.

ACES use in isoelectric focusing of proteins has also been documented.Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." "Electrophoresis", 9(2):65-73. Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE system.Strating, H., and Clarke, A.J., "Differentiation of bacterial autolysins by zymogram analysis." "Anal. Biochem.", 291(1):149-154. Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.Tunnicliff, G., and Smith, J.A., "Competitive inhibition of γ-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-ε-ethanesulfonic acid and related buffer." "J. Neurochem.", 36(3):1122-1126.

References