:Bis-tris methane

{{chembox

| Verifiedfields = changed

| Watchedfields = changed

| verifiedrevid = 477373048

| ImageFile = BisTris.png

| ImageFile_Ref = {{chemboximage|correct|??}}

| ImageSize = 160

| ImageName = Skeletal formula of bis-tris methane

| PIN = 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol{{cite web|title=Bistris - Compound Summary|url=https://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=81462&loc=ec_rcs#x291|work=PubChem Compound|publisher=National Center for Biotechnology Information|access-date=9 April 2012|location=USA|date=23 June 2005|at=Identification}}

| OtherNames = {{Unbulleted list|Bis-(2-hydroxy-ethyl)-amino-tris(hydroxymethyl)-methane|2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol|2,2-Bis(hydroxymethyl)-2,2,2-nitrilotriethanol}}

|Section1={{Chembox Identifiers

| CASNo = 6976-37-0

| CASNo_Ref = {{cascite|correct|CAS}}

| UNII_Ref = {{fdacite|correct|FDA}}

| UNII = Q1XC3631CP

| PubChem = 81462

| ChemSpiderID = 73505

| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}

| EINECS = 230-237-7

| MeSHName = Bistris

| ChEBI = 41250

| ChEBI_Ref = {{ebicite|correct|EBI}}

| RTECS =

| Beilstein = 2205275

| Gmelin = 4519

| SMILES = OCCN(CCO)C(CO)(CO)CO

| StdInChI = 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

| StdInChI_Ref = {{stdinchicite|correct|chemspider}}

| StdInChIKey = OWMVSZAMULFTJU-UHFFFAOYSA-N

| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}

}}

|Section2={{Chembox Properties

| C=8 | H=19 | N=1 | O=5

| Appearance = White crystals

| Odor = Odourless

| MeltingPtC = 102 to 103

| Solubility = 209.2 g L⁻¹ (at 20 °C)

| pKa = 6.46

| pKb = 7.54

| LambdaMax = 280 nm

| Absorbance = 0.15

}}

|Section3={{Chembox Hazards

| GHSPictograms = {{gHS exclamation mark}}

| GHSSignalWord = WARNING

| HPhrases = {{h-phrases|315|319|335}}

| PPhrases = {{p-phrases|261|305+351+338}}

| NFPA-H = 2

| NFPA-F = 0

| NFPA-R = 0

}}

|Section4={{Chembox Related

| OtherFunction_label = alkanols

| OtherFunction = {{unbulleted list|N-Methylethanolamine|Dimethylethanolamine|Diethylethanolamine|Diethanolamine|N,N-Diisopropylaminoethanol|Methyl diethanolamine|Triethanolamine|Meglumine}}

| OtherCompounds = Diethylhydroxylamine

}}

}}

Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C. It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.{{Cite journal|last1=Ferreira|first1=Carlos M. H.|last2=Pinto|first2=Isabel S. S.|last3=Soares|first3=Eduardo V.|last4=Soares|first4=Helena M. V. M.|date=2015|title=(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review|url=https://xlink.rsc.org/?DOI=C4RA15453C|journal=RSC Advances|language=en|volume=5|issue=39|pages=30989–31003|doi=10.1039/c4ra15453c|issn=2046-2069|hdl=10400.22/7404|hdl-access=free}}