:Clavaminate synthase

{{Infobox enzyme

| Name = Clavaminate synthase

| EC_number = 1.14.11.21

| CAS_number = 122799-56-8

| GO_code =

| image =

| width =

| caption =

}}

Clavaminate synthase ({{EC number|1.14.11.21}}, clavaminate synthase 2, clavaminic acid synthase) is an enzyme with systematic name deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating).{{cite journal | vauthors = Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA | title = Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction | journal = Biochemistry | volume = 30 | issue = 8 | pages = 2281–92 | date = February 1991 | pmid = 1998687 | doi = 10.1021/bi00222a034 }}{{cite journal | title = Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation |author1 = Zhou, J. |author2 =Gunsior, M. |author3 =Bachmann, B.O. |author4 =Townsend, C.A. |author5 =Solomon, E.I. |author-link5 = Edward I. Solomon |journal = J. Am. Chem. Soc. |year = 1998 |volume = 120 |pages = 13539–13540 |doi=10.1021/ja983534x}}{{cite journal | vauthors = Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, Schofield CJ | title = Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase | journal = Nature Structural Biology | volume = 7 | issue = 2 | pages = 127–33 | date = February 2000 | pmid = 10655615 | doi = 10.1038/72398 | author-link6 = Christopher J. Schofield }}{{cite journal | vauthors = Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI | title = Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities | journal = Journal of the American Chemical Society | volume = 123 | issue = 30 | pages = 7388–98 | date = August 2001 | pmid = 11472170 | doi = 10.1021/ja004025+| author-link6 = Edward I. Solomon }}{{cite journal | vauthors = Townsend CA | title = New reactions in clavulanic acid biosynthesis | journal = Current Opinion in Chemical Biology | volume = 6 | issue = 5 | pages = 583–9 | date = October 2002 | pmid = 12413541 | doi = 10.1016/S1367-5931(02)00392-7 }} This enzyme catalyses the following chemical reaction

:(1) deoxyamidinoproclavaminate + 2-oxoglutarate + O₂ $\backslash rightleftharpoons$ amidinoproclavaminate + succinate + CO₂

:(2) proclavaminate + 2-oxoglutarate + O₂ $\backslash rightleftharpoons$ dihydroclavaminate + succinate + CO₂ + H₂O

:(3) dihydroclavaminate + 2-oxoglutarate + O₂ $\backslash rightleftharpoons$ clavaminate + succinate + CO₂ + H₂O

Clavaminate synthase contains nonheme iron.