:Hygromycin B 4-O-kinase

{{Infobox enzyme

| Name = Hygromycin B 4-O-kinase

| EC_number = 2.7.1.163

| CAS_number =

| GO_code =

| image =

| width =

| caption =

}}

Hygromycin B 4-O-kinase ({{EC number|2.7.1.163}}, hygromycin-B kinase) is an enzyme with systematic name ATP:hygromycin-B 4-O-phosphotransferase.{{cite journal | vauthors = Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW | title = Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli | journal = Antimicrobial Agents and Chemotherapy | volume = 24 | issue = 5 | pages = 689–95 | date = November 1983 | pmid = 6318654 | pmc = 185926 | doi = 10.1128/aac.24.5.689 }} This enzyme catalyses the following chemical reaction

: ATP + hygromycin B $\backslash rightleftharpoons$ ADP + 4-O-phosphohygromycin B

Phosphorylates the antibiotic hygromycin B.