:Pentachlorophenol monooxygenase

{{Short description|Class of enzymes}}

{{Infobox enzyme

| Name = Pentachlorophenol monooxygenase

| EC_number = 1.14.13.50

| CAS_number = 136111-57-4

| GO_code =

| image =

| width =

| caption =

}}

Pentachlorophenol monooxygenase ({{EC number|1.14.13.50}}, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol 4-monooxygenase, PCP hydroxylase, pentachlorophenol hydroxylase, PcpB, PCB 4-monooxygenase, PCB4MO) is an enzyme with systematic name pentachlorophenol,NADPH:oxygen oxidoreductase (hydroxylating, dechlorinating).{{cite journal | vauthors = Schenk T, Müller R, Mörsberger F, Otto MK, Lingens F | title = Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790 | journal = Journal of Bacteriology | volume = 171 | issue = 10 | pages = 5487–91 | date = October 1989 | doi = 10.1128/jb.171.10.5487-5491.1989 | pmid = 2793827 | pmc = 210388 }}{{cite journal | vauthors = Xun L, Topp E, Orser CS | title = Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries | journal = Journal of Bacteriology | volume = 174 | issue = 9 | pages = 2898–902 | date = May 1992 | doi = 10.1128/jb.174.9.2898-2902.1992 | pmid = 1569020 | pmc = 205942 }}{{cite journal | vauthors = Xun L, Topp E, Orser CS | title = Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase | journal = Journal of Bacteriology | volume = 174 | issue = 17 | pages = 5745–7 | date = September 1992 | doi = 10.1128/jb.174.17.5745-5747.1992 | pmid = 1512208 | pmc = 206524 }}{{cite journal | vauthors = Lange CC, Schneider BJ, Orser CS | title = Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723 | journal = Biochemical and Biophysical Research Communications | volume = 219 | issue = 1 | pages = 146–9 | date = February 1996 | pmid = 8619798 | doi = 10.1006/bbrc.1996.0196 }}{{cite journal | vauthors = Nakamura T, Motoyama T, Hirono S, Yamaguchi I | title = Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase | journal = Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | volume = 1700 | issue = 2 | pages = 151–9 | date = August 2004 | pmid = 15262224 | doi = 10.1016/j.bbapap.2004.04.008 }} This enzyme catalyses the following chemical reaction

: (1) pentachlorophenol + 2 NADPH + H⁺ + O2 $\backslash rightleftharpoons$ 2,3,5,6-tetrachlorohydroquinone + 2 NADP⁺ + chloride + H₂O

: (2) 2,3,5,6-tetrachlorophenol + NADPH + H⁺ + O2 $\backslash rightleftharpoons$ 2,3,5,6-tetrachlorohydroquinone + NADP⁺ + H₂O

Pentachlorophenol monooxygenase is a flavoprotein (FAD).