:TRNA pseudouridine13 synthase

{{Infobox enzyme

| Name = tRNA pseudouridine¹³ synthase

| EC_number = 5.4.99.27

| CAS_number = 430429-15-5

| GO_code =

| image =

| width =

| caption =

}}{{Enzyme-stub}}

tRNA pseudouridine¹³ synthase ({{EnzExplorer|5.4.99.27}}, TruD, YgbO, tRNA PSI13 synthase, RNA:PSI-synthase Pus7p, Pus7p, RNA:pseudouridine-synthase Pus7p, Pus7 protein) is an enzyme with systematic name tRNA-uridine¹³ uracil mutase.{{cite journal | vauthors = Ericsson UB, Nordlund P, Hallberg BM | title = X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold | journal = FEBS Letters | volume = 565 | issue = 1–3 | pages = 59–64 | date = May 2004 | pmid = 15135053 | doi = 10.1016/j.febslet.2004.03.085 | s2cid = 31602560 }}{{cite journal | vauthors = Chan CM, Huang RH | title = Enzymatic characterization and mutational studies of TruD--the fifth family of pseudouridine synthases | journal = Archives of Biochemistry and Biophysics | volume = 489 | issue = 1–2 | pages = 15–9 | date = September 2009 | pmid = 19664587 | doi = 10.1016/j.abb.2009.07.023 }}{{cite journal | vauthors = Kaya Y, Ofengand J | title = A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya | journal = RNA | volume = 9 | issue = 6 | pages = 711–21 | date = June 2003 | pmid = 12756329 | pmc = 1370438 | doi = 10.1261/rna.5230603 }}{{cite journal | vauthors = Behm-Ansmant I, Urban A, Ma X, Yu YT, Motorin Y, Branlant C | title = The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs | journal = RNA | volume = 9 | issue = 11 | pages = 1371–82 | date = November 2003 | pmid = 14561887 | pmc = 1287059 | doi = 10.1261/rna.5520403 }}{{cite journal | vauthors = Urban A, Behm-Ansmant I, Branlant C, Motorin Y | title = RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p | journal = The Journal of Biological Chemistry | volume = 284 | issue = 9 | pages = 5845–58 | date = February 2009 | pmid = 19114708 | doi = 10.1074/jbc.m807986200 | s2cid = 19586610 | url = https://hal.archives-ouvertes.fr/hal-01738433/file/Urban%20et%20al.%2C%20JBC%2C%202009.pdf | doi-access = free }} This enzyme catalyses the following chemical reaction

: tRNA uridine¹³ $\backslash rightleftharpoons$ tRNA pseudouridine¹³

Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine¹³ in tRNA.