:TRNA pseudouridine65 synthase

{{Infobox enzyme

| Name = tRNA pseudouridine⁶⁵ synthase

| EC_number = 5.4.99.26

| CAS_number = 430429-15-5

| GO_code =

| image =

| width =

| caption =

}}

tRNA pseudouridine⁶⁵ synthase ({{EnzExplorer|5.4.99.26}}, TruC, YqcB) is an enzyme with systematic name tRNA-uridine⁶⁵ uracil mutase.{{cite journal | vauthors = Del Campo M, Kaya Y, Ofengand J | title = Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli | journal = RNA | volume = 7 | issue = 11 | pages = 1603–15 | date = November 2001 | pmid = 11720289 | pmc = 1370202 }} This enzyme catalyses the following chemical reaction

: tRNA uridine⁶⁵ $\backslash rightleftharpoons$ tRNA pseudouridine⁶⁵

TruC specifically modifies uridines at positions 65 in tRNA.