2016 U.S. Open Cup

{{main article|2016 U.S. Open Cup qualification}}

All United States Division I (MLS), Division II (NASL), and Division III (USL) teams qualified automatically, except teams that are majority owned by a higher-level outdoor professional league team. This rule prevented United Soccer League teams Bethlehem Steel FC, LA Galaxy II, New York Red Bulls II, Orlando City B, Portland Timbers 2, Real Monarchs, Seattle Sounders FC 2 and Swope Park Rangers from competing. New York Cosmos B (NPSL) also stated that they would not enter the competition. The Rio Grande Valley FC Toros were ruled ineligible at the request of the Houston Dynamo. The New York Athletic Club (NPSL) withdrew before the access list was announced on February 5.

{{#invoke:Build bracket|main

| rounds = 3

| col1-matches = 3,5.5,8,10,12.5,14.5,17,19.5,22,24.5,26.5,29,31.5,34,36.5,38.5,41,43,45.5,47.5,49.5,52,54

| col2-matches = 3,5.5,8,10,13.5,17,19.5,22,24.5,26.5,29,31.5,34,36.5,38.5,41,43,46.5,49.5,52,54

| col3-matches = 3,5.5,9,13.5,17,19.5,22,25.5,29,31.5,34,37.5,42,48,53

| col1-col2-paths = 3-3,5.5-5.5,8-8,10-10,(12.5,14.5)-13.5,17-17,19.5-19.5,22-22,24.5-24.5,26.5-26.5,29-29,31.5-31.5,34-34,36.5-36.5,38.5-38.5,41-41,43-43,(45.5,47.5)-46.5,49.5-49.5,52-52,54-54

| col2-col3-paths = 3-3,5.5-5.5,(8,10)-9,13.5-13.5,17-17,19.5-19.5,22-22,(24.5,26.5)-25.5,29-29,31.5-31.5,34-34,(36.5,38.5)-37.5,(41,43)-42,(46.5,49.5)-48,(52,54)-53

| RD1=First round
May 11

| RD2=Second round
May 18–19

| RD3=Third round
June 1

| RD1-seed01= NPSL

| RD1-team01= Richmond Strikers

| RD1-score01= 1

| RD1-seed02= USASA

| RD1-team02= Aromas Café FC

| RD1-score02= 2*

| RD1-seed03= PDL

| RD1-team03= The Villages SC

| RD1-score03= 1

| RD1-seed04= NPSL

| RD1-team04= Kraze United

| RD1-score04= 0

| RD1-seed05= PDL

| RD1-team05= Reading United

| RD1-score05= 2

| RD1-seed06= NPSL

| RD1-team06= Atlanta Silverbacks Reserves

| RD1-score06= 0

| RD1-seed07= NPSL

| RD1-team07= Fredericksburg FC

| RD1-score07= 1(7)

| RD1-seed08= USASA

| RD1-team08= West Chester United

| RD1-score08= 1(8)

| RD1-seed09= USASA

| RD1-team09= {{nowrap|New York Pancyprian-Freedoms}}

| RD1-score09= 2

| RD1-seed10= PDL

| RD1-team10= New York Red Bulls U-23

| RD1-score10= 1

| RD1-seed11= NPSL

| RD1-team11= Clarkstown SC Eagles

| RD1-score11= 1

| RD1-seed12= PDL

| RD1-team12= Jersey Express

| RD1-score12= 2

| RD1-seed13= PDL

| RD1-team13= Charlotte Eagles

| RD1-score13= 3*

| RD1-seed14= NPSL

| RD1-team14= Myrtle Beach Mutiny

| RD1-score14= 2

| RD1-seed15= NPSL

| RD1-team15= Miami Fusion FC

| RD1-score15= 4*

| RD1-seed16= USASA

| RD1-team16= Boca Raton Football Club

| RD1-score16= 1

| RD1-seed17= NPSL

| RD1-team17= Indiana Fire

| RD1-score17= 1

| RD1-seed18= PDL

| RD1-team18= Cincinnati Dutch Lions

| RD1-score18= 0

| RD1-seed19= USASA

| RD1-team19= Southie FC

| RD1-score19= 2

| RD1-seed20= PDL

| RD1-team20= Seacoast United Phantoms

| RD1-score20= 0

| RD1-seed21= PDL

| RD1-team21= Long Island Rough Riders

| RD1-score21= 1

| RD1-seed22= USASA

| RD1-team22= Lansdowne Bhoys FC

| RD1-score22= 2

| RD1-seed23= PDL

| RD1-team23= Michigan Bucks

| RD1-score23= 0(3)

| RD1-seed24= NPSL

| RD1-team24= Detroit City FC

| RD1-score24= 0(4)

| RD1-seed25= NPSL

| RD1-team25= AFC Cleveland

| RD1-score25= 1

| RD1-seed26= PDL

| RD1-team26= GPS Portland Phoenix

| RD1-score26= 0

| RD1-seed27= PDL

| RD1-team27= Mississippi Brilla

| RD1-score27= 2

| RD1-seed28= USASA

| RD1-team28= Motagua New Orleans

| RD1-score28= 0

| RD1-seed29= NPSL

| RD1-team29= FC Wichita

| RD1-score29= 1

| RD1-seed30= PDL

| RD1-team30= Des Moines Menace

| RD1-score30= 2

| RD1-seed31= USASA

| RD1-team31= NTX Rayados

| RD1-score31= {{abbr|for|forfeit}}

| RD1-seed32= NPSL

| RD1-team32= Corinthians FC of San Antonio

| RD1-score32= –

| RD1-seed33= PDL

| RD1-team33= FC Tucson

| RD1-score33= 3

| RD1-seed34= USASA

| RD1-team34= San Nicolas FC

| RD1-score34= 0

| RD1-seed35= PDL

| RD1-team35= Albuquerque Sol FC

| RD1-score35= 0

| RD1-seed36= USSSA

| RD1-team36= Harpo's FC

| RD1-score36= 2

| RD1-seed37= PDL

| RD1-team37= Portland Timbers U-23

| RD1-score37=0

| RD1-seed38= USASA

| RD1-team38= La Máquina FC

| RD1-score38=2

| RD1-seed39= NPSL

| RD1-team39= Sacramento Gold

| RD1-score39= 3

| RD1-seed40= PDL

| RD1-team40= Burlingame Dragons FC

| RD1-score40= 1

| RD1-seed41= PDL

| RD1-team41= Ventura County Fusion

| RD1-score41= {{abbr|for|forfeit}}

| RD1-seed42= USASA

| RD1-team42= L.A. Wolves FC

| RD1-score42= –

| RD1-seed43= PDL

| RD1-team43= Seattle Sounders FC U-23

| RD1-score43= 3*

| RD1-seed44= USASA

| RD1-team44= Outbreak FC

| RD1-score44= 1

| RD1-seed45= USCS

| RD1-team45= San Francisco City FC

| RD1-score45= 0

| RD1-seed46= NPSL

| RD1-team46= CD Aguiluchos USA

| RD1-score46= 3

| RD2-seed01= USL

| RD2-team01= Richmond Kickers

| RD2-score01= 4

| RD2-seed02= USASA

| RD2-team02= Aromas Café FC

| RD2-score02= 0

| RD2-seed03= USL

| RD2-team03= Charleston Battery

| RD2-score03= –

| RD2-seed04= PDL

| RD2-team04= The Villages SC

| RD2-score04= {{abbr|for|forfeit}}

| RD2-seed05= NPSL

| RD2-team05= Chattanooga FC

| RD2-score05= 0(4)

| RD2-seed06= PDL

| RD2-team06= Reading United

| RD2-score06= 0(1)

| RD2-seed07= USASA

| RD2-team07= West Chester United

| RD2-score07= 0

| RD2-seed08= USL

| RD2-team08= Harrisburg City Islanders

| RD2-score08= 2

| RD2-seed09= USASA

| RD2-team09= New York Pancyprian-Freedoms

| RD2-score09=0

| RD2-seed10= PDL

| RD2-team10= Jersey Express

| RD2-score10=1

| RD2-seed11= PDL

| RD2-team11= Charlotte Eagles

| RD2-score11= 0

| RD2-seed12= USL

| RD2-team12= Charlotte Independence

| RD2-score12= 2

| RD2-seed13= USL

| RD2-team13= Wilmington Hammerheads FC

| RD2-score13= 6

| RD2-seed14= NPSL

| RD2-team14= Miami Fusion FC

| RD2-score14= 0

| RD2-seed15= USL

| RD2-team15= FC Cincinnati

| RD2-score15= 2

| RD2-seed16= NPSL

| RD2-team16= Indiana Fire

| RD2-score16= 1

| RD2-seed17= USL

| RD2-team17= Rochester Rhinos

| RD2-score17= 7

| RD2-seed18= USASA

| RD2-team18= Southie FC

| RD2-score18= 0

| RD2-seed19= USL

| RD2-team19= Pittsburgh Riverhounds

| RD2-score19= 0

| RD2-seed20= USASA

| RD2-team20= Lansdowne Bhoys FC

| RD2-score20= 2

| RD2-seed21= USL

| RD2-team21= Louisville City FC

| RD2-score21= 1(3)

| RD2-seed22= NPSL

| RD2-team22= Detroit City FC

| RD2-score22= 1(1)

| RD2-seed23= USL

| RD2-team23= Saint Louis FC

| RD2-score23= 2

| RD2-seed24= NPSL

| RD2-team24= AFC Cleveland

| RD2-score24= 0

| RD2-seed25= PDL

| RD2-team25= Mississippi Brilla

| RD2-score25= 0(9)

| RD2-seed26= USL

| RD2-team26= Oklahoma City Energy FC

| RD2-score26= 0(10)

| RD2-seed27= PDL

| RD2-team27= Des Moines Menace

| RD2-score27= 2

| RD2-seed28= USL

| RD2-team28= Tulsa Roughnecks FC

| RD2-score28= 0

| RD2-seed29= USL

| RD2-team29= San Antonio FC

| RD2-score29= 3

| RD2-seed30= NPSL

| RD2-team30= Corinthians FC of San Antonio

| RD2-score30= 1

| RD2-seed31= PDL

| RD2-team31= FC Tucson

| RD2-score31= 0

| RD2-seed32= USL

| RD2-team32= Arizona United SC

| RD2-score32= 5

| RD2-seed33= USL

| RD2-team33= {{nowrap|Colorado Springs Switchbacks FC}}

| RD2-score33= 1

| RD2-seed34= USSSA

| RD2-team34= Harpo's FC

| RD2-score34= 0

| RD2-seed35= USASA

| RD2-team35= La Máquina FC

| RD2-score35= 2

| RD2-seed36= NPSL

| RD2-team36= Sacramento Gold

| RD2-score36= 0

| RD2-seed37= USASA

| RD2-team37= L.A. Wolves FC

| RD2-score37= 1(4)

| RD2-seed38= USL

| RD2-team38= Orange County Blues FC

| RD2-score38= 1(2)

| RD2-seed39= PDL

| RD2-team39= Kitsap Pumas

| RD2-score39= 1

| RD2-seed40= PDL

| RD2-team40= Seattle Sounders FC U-23

| RD2-score40= 0

| RD2-seed41= USL

| RD2-team41= Sacramento Republic FC

| RD2-score41= 5

| RD2-seed42= NPSL

| RD2-team42= CD Aguiluchos USA

| RD2-score42= 0

| RD3-seed01= NASL

| RD3-team01= {{nowrap|Fort Lauderdale Strikers}}

| RD3-score01= 1(2)

| RD3-seed02= USL

| RD3-team02= Richmond Kickers

| RD3-score02= 1(0)

| RD3-seed03= NASL

| RD3-team03= Jacksonville Armada FC

| RD3-score03= 2*

| RD3-seed04= USL

| RD3-team04= Charleston Battery

| RD3-score04= 1

| RD3-seed05= NPSL

| RD3-team05= Chattanooga FC

| RD3-score05= 1

| RD3-seed06= USL

| RD3-team06= Harrisburg City Islanders

| RD3-score06= 2

| RD3-seed07= NASL

| RD3-team07= New York Cosmos

| RD3-score07= 2

| RD3-seed08= PDL

| RD3-team08= Jersey Express

| RD3-score08= 0

| RD3-seed09= NASL

| RD3-team09= Carolina RailHawks

| RD3-score09= 5*

| RD3-seed10= USL

| RD3-team10= Charlotte Independence

| RD3-score10= 0

| RD3-seed11= NASL

| RD3-team11= Miami FC

| RD3-score11= 1

| RD3-seed12= USL

| RD3-team12= Wilmington Hammerheads FC

| RD3-score12= 2

| RD3-seed13= NASL

| RD3-team13= Tampa Bay Rowdies

| RD3-score13= 1

| RD3-seed14= USL

| RD3-team14= FC Cincinnati

| RD3-score14= 0

| RD3-seed15= USL

| RD3-team15= Rochester Rhinos

| RD3-score15= 2

| RD3-seed16= USASA

| RD3-team16= Lansdowne Bhoys FC

| RD3-score16= 0

| RD3-seed17= NASL

| RD3-team17= Indy Eleven

| RD3-score17= 2

| RD3-seed18= USL

| RD3-team18= Louisville City FC

| RD3-score18= 1

| RD3-seed19= USL

| RD3-team19= Saint Louis FC

| RD3-score19= 0

| RD3-seed20= NASL

| RD3-team20= Minnesota United FC

| RD3-score20= 2

| RD3-seed21= NASL

| RD3-team21= Rayo OKC

| RD3-score21= 1

| RD3-seed22= USL

| RD3-team22= Oklahoma City Energy FC

| RD3-score22= 2*

| RD3-seed23= PDL

| RD3-team23= Des Moines Menace

| RD3-score23= 1

| RD3-seed24= USL

| RD3-team24= San Antonio FC

| RD3-score24= 2

| RD3-seed25= USL

| RD3-team25= Arizona United SC

| RD3-score25= 0

| RD3-seed26= USL

| RD3-team26= {{nowrap|Colorado Springs Switchbacks FC}}

| RD3-score26= 3

| RD3-seed27= USASA

| RD3-team27= La Máquina FC

| RD3-score27= 2

| RD3-seed28= USASA

| RD3-team28= L.A. Wolves FC

| RD3-score28= 0

| RD3-seed29= PDL

| RD3-team29= Kitsap Pumas

| RD3-score29= 3

| RD3-seed30= USL

| RD3-team30= Sacramento Republic FC

| RD3-score30= 1

}}Host team listed first
Bold = winner
* = after extra time, ( ) = penalty shootout score

Draw announced April 6. All times local to game site.

{{Football box collapsible

|date = May 11

|time = 5:00 PM EDT

|team1 = The Villages SC (PDL)

|score = 1–0

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-kraze-united-sc-at-the-villages-sc USSF Report]
[http://thevillagessc.com/u-s-open-cup-roundup-the-villages-sc-nets-late-winner/ TVSC Report]

|team2 = Kraze United (NPSL)

|goals1 = Dias {{goal|90+2}}

|goals2 =

|stadium = The Villages Polo Field

|location = The Villages, Florida

|attendance =

|referee = Alexander DeRostaing

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 5:00 PM EDT

|team1 = Richmond Strikers (NPSL)

|score = 1–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-aromas-cafe-fc-at-richmond-strikers USSF Report]

|team2 = Aromas Café FC (USASA)

|goals1 = Bates {{goal|73|pen.}}

|goals2 = Clare {{goal|47}}
van Yahres {{goal|105}}

|aet = y

|stadium = Striker Park

|location = Glen Allen, Virginia

|attendance =

|referee = Matthew Franz

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 5:30 PM EDT

|team1 = Fredericksburg FC (NPSL)

|score = 1–1

|penaltyscore = 7–8

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-west-chester-united-at-fredericksburg-fc USSF Report]

|team2 = West Chester United (USASA)

|goals1 = Zinkam {{goal|87|pen.}}

|goals2 = Sales {{goal}}

|aet = y

|stadium = Battleground Stadium

|location = Fredericksburg, Virginia

|attendance =

|referee = Mark Gorak

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM EDT

|team1 = Miami Fusion FC (NPSL)

|score = 4–1

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-boca-raton-football-club-at-miami-fusion-fc USSF Report]

|team2 = Boca Raton FC (USASA)

|goals1 = Santamaria {{goal|52}}
Faccini {{goal|103||110}}
Marugo {{goal|107}}

|goals2 = Taboada {{goal|67}}

|aet = y

|stadium = North Miami Athletic Stadium

|location = North Miami Beach, Florida

|attendance =

|referee = Andres Pfefferkorn

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM EDT

|team1 = Indy Eleven NPSL (NPSL)

|score = 1–0

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-cincinnati-dutch-lions-at-indy-eleven-npsl USSF Report]
[http://www.indyeleven.com/news/2016/05/11/elevennpsl-recap-051116vcdl IND Report]
[http://cdlfc.com/2016/05/cdl-drops-us-open-cup-match-1-0-vs-indy-eleven-npsl/ CDL Report]

|team2 = Cincinnati Dutch Lions (PDL)

|goals1 = Mitchell {{goal|17}}

|goals2 =

|stadium = Grand Park Championship Field

|location = Westfield, Indiana

|attendance =

|referee = Aaron Hernandez

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM EDT

|team1 = Clarkstown SC Eagles (NPSL)

|score = 1–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-jersey-express-at-clarkstown-sc-eagles USSF Report]

|team2 = Jersey Express S.C. (PDL)

|goals1 = Bardic {{goal|90+1}}

|goals2 = Correa {{goal|22|pen.|55|pen.}}

|stadium = Torne Valley Sports Complex

|location = Hillburn, New York

|attendance =

|referee = Jared Simons

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM EDT

|team1 = Charlotte Eagles (PDL)

|score = 3–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-myrtle-beach-mutiny-at-charlotte-eagles USSF Report]

|team2 = Myrtle Beach FC (NPSL)

|goals1 = Siaj {{goal|49||59}}
Micaletto {{goal|111|pen.}}

|goals2 = Arambula {{goal|68}}
Ibarrondo {{goal|90+1}}

|aet = y

|stadium = Charlotte Christian School

|location = Charlotte, North Carolina

|attendance =

|referee = Kevin Broadley

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM EDT

|team1 = Reading United A.C. (PDL)

|score = 2–0

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-atlanta-silverbacks-at-reading-united-ac USSF Report]
[http://www.readingunitedac.com/news_article/show/647881?referrer_id= RUAC Report]

|team2 = Atlanta Silverbacks (NPSL)

|goals1 = Bennett {{goal|31}}
Pierrot {{goal|86}}

|goals2 =

|stadium = John Gurski Stadium at Wilson High School

|location = Reading, Pennsylvania

|attendance =

|referee = Ryan Dos Reis

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM EDT

|team1 = Michigan Bucks (PDL)

|score = 0–0

|penaltyscore = 3–4

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-detroit-city-fc-at-michigan-bucks USSF Report]
[http://home.detcityfc.com/news_article/show/647641?referrer_id=1533258 DCFC Report]

|team2 = Detroit City FC (NPSL)

|goals1 =

|goals2 =

|aet = y

|stadium = Oakland University Soccer Field

|location = Rochester, Michigan

|attendance =

|referee = Donovan Noocha

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM EDT

|team1 = Long Island Rough Riders (PDL)

|score =1–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-lansdowne-bhoys-fc-at-long-island-rough-riders USSF Report]

|team2 = Lansdowne Bhoys FC (USASA)

|goals1 = Alfonso {{goal|59}}

|goals2 = Oakley {{goal|7}}
McGuigan {{goal|120+6|pen.}}

|stadium = St. Anthony's High School

|location = South Huntington, New York

|attendance =

|referee = Dave Breckner

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM EDT

|team1 = New York Pancyprian-Freedoms (USASA)

|score = 2–1

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-new-york-red-bulls-u23-at-new-york-pancyprian-freedoms USSF Report]

|team2 = New York Red Bulls U-23 (PDL)

|goals1 = Reyering {{goal|51||66}}

|goals2 = Knudson {{goal|15|pen.}}

|stadium = Belson Stadium

|location = Jamaica, New York

|attendance =

|referee = Rosario Candela

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 8:00 PM EDT

|team1 = Southie FC (USASA)

|score = 2–0

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-seacoast-united-phantoms-at-southie-fc USSF Report]
[http://www.seacoastunited.com/Phantoms/News/915311.html SUP Report]

|team2 = Seacoast United Phantoms (PDL)

|goals1 = Sandeman {{goal|39||59|pen.}}

|goals2 =

|stadium = Harry Della Russo Stadium

|location = Revere, Massachusetts

|attendance =

|referee = Randall Kelley

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 8:00 PM EDT

|team1 = GPS Portland Phoenix (PDL)

|score = 0–1

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-afc-cleveland-at-gps-portland-phoenix USSF Report]
[http://www.gps-maine.com/news_article/show/643046?referrer_id=1858306 GPS Report]
[http://afccleveland.com/news/story.php?id=128 CLE Report]

|team2 = AFC Cleveland (NPSL)

|goals1 =

|goals2 = Manfut {{goal|24}}

|stadium = Deering High School

|location = Portland, Maine

|attendance =

|referee = Michael Dee

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM CDT

|team1 = Mississippi Brilla (PDL)

|score = 2–0

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-cd-motagua-of-new-orleans-at-mississippi-brilla USSF Report]
[http://brillasoccer.org/blogs/news/116950084-brilla-tops-cd-motagua MBFC Report]

|team2 = Motagua New Orleans (USASA)

|goals1 = Cruz {{goal|28}}
Flores {{goal|43}}

|goals2 =

|stadium = Traceway Park

|location = Clinton, Mississippi

|attendance =

|referee = Leland Grant

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:05 PM CDT

|team1 = FC Wichita (NPSL)

|score = 1–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-des-moines-menace-at-fc-wichita USSF Report]
[http://www.menacesoccer.com/news/?article_id=765 DSM Report]

|team2 = Des Moines Menace (PDL)

|goals1 = Contreras {{goal|46}}

|goals2 = Preston {{goal|57}}
Veidman {{goal|85}}

|stadium = Stryker Sports Complex

|location = Wichita, Kansas

|attendance =

|referee = Jon Freemon

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 6:30 PM MDT

|team1 = Albuquerque Sol FC (PDL)

|score = 0–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-harpos-fc-at-albuquerque-sol-fc USSF Report]

|team2 = Harpo's FC (USSSA)

|goals1 =

|goals2 = Salvaggione {{goal|14}}
Wheeler {{goal|33}}

|stadium = St. Pius X High School

|location = Albuquerque, New Mexico

|attendance =

|referee = Steven Schafer

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time =

|team1 = NTX Rayados (USASA)

|score = Forfeit

|note = NTX Rayados disqualified due to not currently being active in a USSF sanctioned league.{{cite web|url=http://www.ussoccer.com/stories/2016/05/11/03/10/160510-usoc-corinthians-sc-of-san-antonio-advance-to-usoc-second-round|title=Corinthians SC of San Antonio Advance to USOC Second Round|publisher=ussoccer.com|date=10 May 2016}}

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-corinthians-fc-of-san-antonio-at-ntx-rayados USSF Report]

|team2 = Corinthians FC of San Antonio (NPSL)

|goals1 =

|goals2 =

|stadium =

|location =

|attendance =

|referee =

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM PDT

|team1 = Sacramento Gold (NPSL)

|score = 3–1

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-burlingame-dragons-at-sacramento-gold USSF Report]
[http://www.sacramentogoldfc.net/#!Second-Half-Surge-Slays-Dragons/c12hj/57343c180cf2d5f002bf41a0 SGFC Report]

|team2 = Burlingame Dragons FC (PDL)

|goals1 = Pina {{goal|56||58}}
Mez {{goal|89}}

|goals2 = Cox {{goal|45+3}}

|stadium = Capital Christian School

|location = Sacramento, California

|attendance =

|referee = Turan Ozdemir

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM PDT

|team1 = Ventura County Fusion (PDL)

|score = Forfeit

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-la-wolves-fc-at-ventura-county-fusion USSF Report]

|team2 = L.A. Wolves FC (USASA)

|goals1 = España {{goal|5}}
Ruiz {{goal|26}}

|goals2 = Chica {{goal|45}}

|note = L.A. protested the result, claiming that Ventura County used a player cup-tied to Cal FC.{{cite web|url=http://thecup.us/2016/05/12/la-wolves-to-file-protest-over-us-open-cup-loss-to-ventura-county-fusion/|title=LA Wolves to file protest over US Open Cup loss to Ventura County Fusion|date=12 May 2016}} On May 13, the protest was upheld and L.A. moved on to the next round.{{cite web|url=http://www.ussoccer.com/stories/2016/05/13/18/11/160513-usoc-la-wolves-fc-protest-upheld-team-advances-to-second-round|title=L.A. Wolves FC Protest Upheld; Team Advances to Second Round|date=13 May 2016}}

|stadium = Ventura College Sportsplex

|location = Ventura, California

|attendance =

|referee = Reed Christy

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:00 PM PDT

|team1 = San Francisco City FC (USCS)

|score = 0–3

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-cd-aguiluchos-usa-at-san-francisco-city-fc USSF Report]

|team2 = CD Aguiluchos USA (NPSL)

|goals1 =

|goals2 = Rawsley {{goal|64}}
Santiago {{goal|88}}
Guerra {{goal|90+1}}

|stadium = Beach Chalet #4

|location = San Francisco, California

|attendance =

|referee = Michael Samaan

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM MST

|team1 = FC Tucson (PDL)

|score = 3–0

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-san-nicolas-fc-at-fc-tucson USSF Report]
[http://fctucson.com/team/team-earns-shot-at-az-united-in-open-cup-win/ FCT Report]

|team2 = San Nicolas FC (USASA)

|goals1 = Papa {{goal|6}}
Minh Vu {{goal|32|pen.|57}}

|goals2 =

|stadium = Kino North Stadium

|location = Tucson, Arizona

|attendance =

|referee = Anthony Bersano

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM PDT

|team1 = Portland Timbers U23s (PDL)

|score = 0–2

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-la-maquina-at-portland-timbers-u23s USSF Report]
[https://www.timbers.com/news/us-open-cup-match-recap-portland-timbers-u-23s-0-la-maquina-2 PTU23 Report]

|team2 = La Máquina FC (USASA)

|goals1 =

|goals2 = Velazquez {{goal|6}}
Barboa {{goal|90+3}}

|stadium = Providence Park

|location = Portland, Oregon

|attendance =

|referee = Farhad Dadkho

|stack = yes

}}

{{Football box collapsible

|date = May 11

|time = 7:30 PM PDT

|team1 = Seattle Sounders FC U-23 (PDL)

|score = 3–1

|report =[http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/first-round/160511-usoc-first-round-outbreak-fc-at-sounders-fc-u-23s USSF Report]{{dead link|date=September 2017 |bot=InternetArchiveBot |fix-attempted=yes }}
[http://soundersu23.com/home/915247.html SSU23 Report]

|team2 = Outbreak FC (USASA)

|aet = y

|goals1 = Maguraushe {{goal|42}}
Olsen {{goal|115}}
Apollon {{goal|120}}

|goals2 = Soria {{goal|29}}

|stadium = Bonney Lake High School

|location = Bonney Lake, Washington

|attendance =

|referee = Jeff Arthurholtz

|stack = no

}}

{{reflist|group=note}}

Draw announced April 6. All times local to game site.

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = FC Cincinnati (USL)

|score = 2–1

|report = [https://web.archive.org/web/20160611123841/http://www.fccincinnati.com/fcc-edges-indy-eleven-in-inaugural-open-cup-win/ Report]

|team2 = Indy Eleven NPSL (NPSL)

|goals1 = Cummings {{goal|36|pen.}}
McLaughlin {{goal|61}}

|goals2 = Mitchell {{goal|39}}

|stadium = Nippert Stadium

|location = Cincinnati, Ohio

|attendance = 8,668

|referee = Aaron Mitchell

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Richmond Kickers (USL)

|score = 4–0

|report =

|team2 = Aromas Café FC (USASA)

|goals1 = Jane {{goal|2}}
Roberts {{goal|25}}
Grant {{goal|48}}
Luiz Fernando {{goal|83}}

|goals2 =

|stadium = City Stadium

|location = Richmond, Virginia

|attendance = 1,428

|referee = JT Alukonis

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Charlotte Eagles (PDL)

|score = 0–2

|report = [http://www.charlotteindependence.com/independence-top-eagles-2-0-in-first-u-s-open-cup-match-of-2016/ Report]

|team2 = Charlotte Independence (USL)

|goals1 =

|goals2 = Hellmann {{goal|44}}
Herrera {{goal|50}}

|stadium = Charlotte Christian School

|location = Charlotte, North Carolina

|attendance =

|referee = Gustavo Solorio

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Wilmington Hammerheads FC (USL)

|score = 6–0

|report = [http://www.wilmingtonhammerheads.com/wilmington-sends-miami-fusion-home-goose-egg-winning-6-0/ Report]

|team2 = Miami Fusion FC (NPSL)

|goals1 = Martz {{goal|6||19||32}}
Moose {{goal|9}}
Parker {{goal|78||90+2}}

|goals2 =

|stadium = Legion Stadium

|location = Wilmington, North Carolina

|attendance =

|referee = Corson Fidler

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Louisville City FC (USL)

|score = 1–1

|aet = yes

|penaltyscore = 3–1

|report = [https://web.archive.org/web/20160519234409/http://louisvillecityfc.com/recap-2/ Report]

|team2 = Detroit City FC (NPSL)

|goals2 = Harris {{goal|104}}

|goals1 = Ilić {{goal|115}}

|stadium = Owsley B. Frazier Stadium

|location = Louisville, Kentucky

|attendance =

|referee = Brandon Artis

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Rochester Rhinos (USL)

|score = 7–0

|report = [https://web.archive.org/web/20160602073724/http://www.rhinossoccer.com/news_article/show/650163?referrer_id=2439299]

|team2 = Southie FC (USASA)

|goals1 = Volesky {{goal|6|pen.}}
Fortune {{goal|9}}
Ugarte {{goal|27}}
Samuels {{goal|42||44}}
Own Goal {{goal|48}}
Samuels {{goal|76}}

|goals2 =

|stadium = Rochester Rhinos Stadium

|location = Rochester, New York

|attendance = 1,371{{cite web |url=https://www.democratandchronicle.com/story/sports/soccer/2016/05/18/rhinos-crush-southie-fc-7-0-second-round-cup-match/84567810/|title=Rhinos crush Southie FC 7-0 in second-round Cup match|website= Rochester Democrat and Chronicle}}

|referee = Stephen Montanino

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM EDT

|team1 = Pittsburgh Riverhounds (USL)

|score = 0–2

|report = [http://riverhounds.com/2016/05/19/hounds-drop-open-cup-match-to-lansdowne-0-2/ Report]

|team2 = Lansdowne Bhoys FC (USASA)

|goals1 =

|goals2 = Purcell {{goal|10}}
Kavanagh {{goal|76}}

|stadium = Highmark Stadium

|location = Pittsburgh, Pennsylvania

|attendance =

|referee = Charles Murphy

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM EDT

|team1 = Charleston Battery (USL)

|score = Forfeit

|report =

|team2 = The Villages SC (PDL)

|goals1 = Marini {{goal|45}}
Tsonis {{goal|82}}

|goals2 = Araujo {{goal|23||67}}

|stadium = MUSC Health Stadium

|location = Charleston, South Carolina

|attendance =

|referee = David Erbacher

|note = Game was tied 2–2 after extra time, and the Villages won 4–2 in penalties. The Villages SC were disqualified from the tournament after the Battery protested their use of a player cup-tied to Boca Raton FC.{{cite web|url=http://www.uslsoccer.com/news_article/show/650557?referrer_id=2333971|title=Battery Advance to Open Cup Third Round|date=19 May 2016}}

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM EDT

|team1 = West Chester United (USASA)

|score = 0–2

|report = [http://cityislanders.com/club-news/city-islanders-advance-to-third-round-of-u-s-open-cup-after-defeating-wcu/ Report]

|team2 = Harrisburg City Islanders (USL)

|goals1 =

|goals2 = Warshaw {{goal|13}}
Bolduc {{goal|49}}

|stadium = West Chester United Soccer Club

|location = West Chester, Pennsylvania

|attendance =

|referee = Rosario Candela

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM EDT

|team1 = Chattanooga FC (NPSL)

|score = 0–0

|aet = yes

|report = [http://thecup.us/2016/05/21/2016-us-open-cup-round-2-chattanooga-fc-outlasts-reading-united-ac-in-pks-in-battle-of-amateur-powers/ Report]

|team2 = Reading United AC (PDL)

|goals1 =

|goals2 =

|stadium = Finley Stadium

|location = Chattanooga, Tennessee

|attendance = 3,641

|referee = Leland Grant

|stack = yes

|penalties1 = {{pengoal}} Bywater
{{pengoal}} Zeca
{{pengoal}} D. Reynolds
{{pengoal}} Carrier

|penaltyscore = 4–1

|penalties2 = {{penmiss}} Aguilar
{{penmiss}} Fountain
{{pengoal}}

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM CDT

|team1 = Mississippi Brilla (PDL)

|score = 0–0

|aet = yes

|penaltyscore = 9–10

|report = [http://www.energyfc.com/game-recap/okc-downs-brilla-fc-on-pks-in-us-open-cup/ Report]

|team2 = Oklahoma City Energy FC (USL)

|goals1 =

|goals2 =

|stadium = Traceway Park

|location = Clinton, Mississippi

|attendance =

|referee = Andrew Musashe

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM CDT

|team1 = Saint Louis FC (USL)

|score = 2–0

|report = [http://www.saintlouisfc.com/sports/msoc/2016-17/releases/20160518ylzfra Report]

|team2 = AFC Cleveland (NPSL)

|goals1 = Hardware {{goal|22}}
Roberts {{goal|77}}

|goals2 =

|stadium = World Wide Technology Soccer Park

|location = Fenton, Missouri

|attendance =

|referee = Elvis Mahmutovic

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM CDT

|team1 = San Antonio FC (USL)

|score = 3–1

|report = [http://www.sanantoniofc.com/news_article/show/650189?referrer_id=2442824 Report]

|team2 = Corinthians FC of San Antonio (NPSL)

|goals1 = Johnson {{goal|3}}
Francois {{goal|70||74}}

|goals2 = Chapman-Page {{goal|47}}

|stadium = Toyota Field

|location = San Antonio, Texas

|attendance = 2,419

|referee = Luis Guardia

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM CDT

|team1 = Des Moines Menace (PDL)

|score = 2–0

|report = [http://www.tulsaroughnecksfc.com/latest-news/roughnecks-knocked-from-us-open-cup-with-2-0-loss Report]

|team2 = Tulsa Roughnecks FC (USL)

|goals1 = Hoek {{goal|70}}
Ibisevic {{goal|82}}

|goals2 =

|stadium = Valley Stadium

|location = West Des Moines, Iowa

|attendance =

|referee = Robert Sorey

|stack =

}}

{{Football box collapsible

|date = May 18

|time = 7:00 PM MDT

|team1 = Colorado Springs Switchbacks FC (USL)

|score = 1–0

|report = [http://switchbacksfc.com/switchbacks-advance-to-3rd-round-of-us-open-cup/ Report]

|team2 = Harpo's FC (USSSA)

|goals1 = Armstrong {{goal|13}}

|goals2 =

|stadium = Switchbacks Stadium

|location = Colorado Springs, Colorado

|attendance =

|referee = Benjamin Oakley

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 6:00 PM PDT

|team1 = La Máquina FC (USASA)

|score = 2–0

|report = [http://www.ussoccer.com/lamar-hunt-us-open-cup/open-cup-tournaments/2016-usoc/second-round/160518-usoc-second-round-sacramento-gold-at-la-maquina Report]

|team2 = Sacramento Gold (NPSL)

|goals1 = Barboa {{goal|70}}
Pérez {{goal|79}}

|goals2 =

|stadium = Boswell Field at Westminster High School

|location = Westminster, California

|attendance =

|referee = Michael Radchuk

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 8:00 PM PDT

|team1 = L.A. Wolves FC (USASA)

|score = 1–1

|report =

|team2 = Orange County Blues FC (USL)

|goals1 = Sanchez {{goal|72}}

|goals2 = Howe {{goal|77}}

|aet = y

|penaltyscore = 4–2

|stadium = Phineas Banning High School

|location = Wilmington, California

|attendance =

|referee = Vincent Apple-Chiarella

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM MST

|team1 = FC Tucson (PDL)

|score = 0–5

|report = [http://arizonaunited.com/recap-united-reaches-third-round-of-u-s-open-cup-after-5-0-win/ Report]

|team2 = Arizona United SC (USL)

|goals1 =

|goals2 = Rooney {{goal|28}}
Tan {{goal|33||68}}
Bardsley {{goal|45+}}
Blackwood {{goal|47}}

|stadium = Kino North Stadium

|location = Tucson, Arizona

|attendance =

|referee = Robi Hullner

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM PDT

|team1 = Kitsap Pumas (PDL)

|score = 1–0

|report =

|team2 = Seattle Sounders FC U-23 (PDL)

|goals1 = Castro {{goal|90}}

|goals2 =

|stadium = Silverdale Stadium

|location = Bremerton, Washington

|attendance =

|referee = Levi Rippy

|stack = yes

}}

{{Football box collapsible

|date = May 18

|time = 7:30 PM PDT

|team1 = Sacramento Republic FC (USL)

|score = 5–0

|report = [http://www.sacrepublicfc.com/news/2016/05/18/srfc-v-cda-recap Report]

|team2 = CD Aguiluchos USA (NPSL)

|goals1 = Motagalvan {{goal|24}}
Pridham {{goal|36}}
Rodriguez {{goal|44}}
Stewart {{goal|45}}
Alvarez {{goal|90}}

|goals2 =

|stadium = Bonney Field

|location = Sacramento, California

|attendance =

|referee = Elijio Arreguin

|stack = no

}}

{{Football box collapsible

|date = May 19

|time = 7:30 PM EDT

|team1 = New York Pancyprian-Freedoms (USASA)

|score = 0–1

|report =

|team2 = Jersey Express S.C. (PDL)

|goals1 =

|goals2 = Otto {{goal|81}}

|stadium = Belson Stadium

|location = Jamaica, New York

|attendance =

|referee = Dave Breckner

|stack = yes

}}

Draw announced May 12.

{{Football box collapsible

|date = June 1

|time = 11:00 AM EDT

|team1 = Fort Lauderdale Strikers (NASL)

|score = 1–1

|report =https://www.espn.com/soccer/match?gameId=448775

|team2 = Richmond Kickers (USL)

|goals1 = Gonzalez {{goal|2}}

|goals2 = Imura {{goal|32}}

|stadium = Broward College South Campus

|location = Pembroke Pines, Florida

|attendance =200

|referee = Caleb Mendez

|aet = yes

|penaltyscore = 2–0

|penalties1= Victor {{pengoal}}
Núñez {{penmiss}}
Santos {{penmiss}}
Moura {{pengoal}}

|penalties2= Imura {{penmiss}}
Paulini {{penmiss}}
Yeisley {{penmiss}}
Nascimento {{penmiss}}

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:00 PM EDT

|team1 = Jacksonville Armada FC (NASL)

|score = 2–1

|report = https://www.espn.com/soccer/match?gameId=448789

|team2 = Charleston Battery (USL)

|goals1 = Otte {{goal|17}}
Keita {{goal|108}}

|goals2 = Tsonis {{goal|22}}

|stadium = Southern Oak Stadium

|location = Jacksonville, Florida

|attendance = 1,200

|referee = Kevin Terry Jr.

|aet= yes

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:00 PM EDT

|team1 = Rochester Rhinos (USL)

|score = 2–0

|report =

|team2 = Lansdowne Bhoys FC (USASA)

|goals1 = Fortune {{goal|59}}
Volesky {{goal|70}}

|goals2 =

|stadium = Rochester Rhinos Stadium

|location = Rochester, New York

|attendance = 4,429

|referee = Jaroslaw Werel

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM EDT

|team1 = Indy Eleven (NASL)

|score = 2–1

|report = [https://web.archive.org/web/20160606195515/http://louisvillecityfc.com/recap-loucity-drops-3rd-round-match/ Report]

|team2 = Louisville City FC (USL)

|goals1 = Gordon {{goal|58}}
Zayed {{goal|68}}

|goals2 = Hoffman {{goal|21}}

|stadium = Carroll Stadium

|location = Indianapolis, Indiana

|attendance = 2,145

|referee = Brandon Artis

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM EDT

|team1 = New York Cosmos (NASL)

|score = 2–0

|report = https://www.espn.com/soccer/match?gameId=448787

|team2 = Jersey Express S.C. (PDL)

|goals1 = Ayoze {{goal|35}}
Orozco {{goal|72}}

|goals2 =

|stadium = Belson Stadium

|location = Jamaica, New York

|attendance = 984

|referee = Dave Breckner

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM EDT

|team1 = Carolina Railhawks (NASL)

|score = 5–0

|aet = yes

|report = https://www.espn.com/soccer/match?gameId=448783

|team2 = Charlotte Independence (USL)

|goals1 = Schuler {{goal|92||115}}
Albadawi {{goal|96}}
Shriver {{goal|99}}
Orlando {{goal|109}}

|goals2 =

|stadium = WakeMed Soccer Park

|location = Cary, North Carolina

|attendance = 1,769

|referee = Neil Barbulescu

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM EDT

|team1 = Chattanooga FC (NPSL)

|score = 1–2

|report =

|team2 = Harrisburg City Islanders (USL)

|goals1 = Yuhaschek {{goal|45+1}}

|goals2 = Wheeler {{goal|71}}
Wilson {{goal|82}}

|stadium = Finley Stadium

|location = Chattanooga, Tennessee

|attendance = 2,595

|referee = Leland Grant

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM EDT

|team1 = Tampa Bay Rowdies (NASL)

|score = 1–0

|report = https://www.espn.com/soccer/match?gameId=448784

|team2 = FC Cincinnati (USL)

|goals1 = Hristov {{goal|11}}

|goals2 =

|stadium = Al Lang Stadium

|location = St. Petersburg, Florida

|attendance = 3,455

|referee = Rubiel Vazquez

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 8:00 PM EDT

|team1 = Miami FC (NASL)

|score = 1–2

|report = https://www.espn.com/soccer/match?gameId=448782

|team2 = Wilmington Hammerheads FC (USL)

|goals1 = Chavez {{goal|52}}

|goals2 = Michaud {{goal|2}}
Moose {{goal|5}}

|stadium = FIU Stadium

|location = Miami, Florida

|attendance = 300

|referee = Guido Gonzales Jr.

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:00 PM CDT

|team1 = Rayo OKC (NASL)

|score = 1–2

|report = https://www.espn.com/soccer/match?gameId=448781

|team2 = Oklahoma City Energy FC (USL)

|goals1 = Forbes {{goal|42}}

|goals2 = Dalgaard {{goal|45}}
Hyland {{goal|107}}

|stadium = Miller Stadium

|location = Yukon, Oklahoma

|attendance = 4,385

|referee = Andrew Musashe

|aet = yes

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:00 PM CDT

|team1 = Saint Louis FC (USL)

|score = 0–2

|report = https://www.espn.com/soccer/match?gameId=448780

|team2 = Minnesota United FC (NASL)

|goals1 =

|goals2 = Venegas {{goal|69}}
Blake {{goal|90+4}}

|stadium = World Wide Technology Soccer Park

|location = Fenton, Missouri

|attendance = 2,358

|referee = Greg Dopka

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 8:00 PM CDT

|team1 = Des Moines Menace (PDL)

|score = 1–2

|report = https://www.sanantoniofc.com/news_article/show/655191?referrer_id=2442824

|team2 = San Antonio FC (USL)

|goals1 = Flath {{goal|60}}

|goals2 = McBride {{goal|72}}
Francois {{goal|81}}

|stadium = Valley Stadium

|location = Des Moines, Iowa

|attendance = 1,754

|referee = Kyle Burkhardt

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:00 PM PDT

|team1 = La Máquina FC (USASA)

|score = 2–0

|report =

|team2 = L.A. Wolves FC (USASA)

|goals1 = Borboa {{goal|17||68}}

|goals2 =

|stadium = Westminster High School

|location = Westminster, California

|attendance =

|referee = Timothy Ford

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 7:30 PM MST

|team1 = Arizona United SC (USL)

|score = 0–3

|report = [http://arizonaunited.com/recap-arizona-united-eliminated-from-u-s-open-cup-following-3-0-loss/ Report]
[http://switchbacksfc.com/switchbacks-earn-us-open-cup-date-with-colorado-rapids/ Report]

|team2 = Colorado Springs Switchbacks FC (USL)

|goals1 =

|goals2 = Vercollone {{goal|39||83}}
King {{goal|59}}

|stadium = Peoria Sports Complex

|location = Peoria, Arizona

|attendance = 1,750

|referee = Ramy Touchan

|stack = yes

}}

{{Football box collapsible

|date = June 1

|time = 8:00 PM PDT

|team1 = Sacramento Republic FC (USL)

|score = 1–3

|report =

|team2 = Kitsap Pumas (PDL)

|goals1 = Iwasa {{goal|61}}

|goals2 = Castro {{goal|60||65}}
Ramos {{goal|63}}

|stadium = Bonney Field

|location = Sacramento, California

|attendance =

|referee = Victor Rivas

|stack =

}}

Draw announced May 19.

{{Football box collapsible

|date = June 14

|time = 7:00 PM MDT

|team1 = Colorado Rapids (1-MLS)

|score = 1–0

|report = [http://www.coloradorapids.com/post/2016/06/14/match-report-rapids-switchbacks-dillon-serna-us-open-cup Report]

|team2 = Colorado Springs Switchbacks FC (3-USL)

|goals1 = Serna {{goal|84}}

|goals2 =

|stadium = Dick's Sporting Goods Park

|location = Commerce City, Colorado

|attendance =

|referee = Baboucarr Jallow (Washington)

|stack = yes

}}

{{Football box collapsible

|date = June 14

|time = 8:00 PM MDT

|team1 = Real Salt Lake (1-MLS)

|score = 2–2

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-14-real-salt-lake-vs-wilmington-hammerheads/boxscore Report]

|team2 = Wilmington Hammerheads FC (3-USL)

|goals1 = Plata {{goal|68}}
Martínez {{goal|86}}

|goals2 = Corboz {{goal|34}}
Michaud {{goal|58}}

|penalties1 = Martínez {{pengoal}}
Plata {{pengoal}}
Morales {{penmiss}}
Wingert {{pengoal}}

|penalties2 = Perone {{penmiss}}
Corboz {{pengoal}}
Barnes-Homer {{penmiss}}
Miller {{penmiss}}

|stadium = Rio Tinto Stadium

|location = Sandy, Utah

|attendance = 17,131

|referee = Victor Rivas (California)

|aet = yes

|penaltyscore = 3–1

|stack = yes

}}

{{Football box collapsible

|date = June 14

|time = 7:30 PM PDT

|team1 = Portland Timbers (1-MLS)

|score = 2–0

|report = [https://www.timbers.com/news/us-open-cup-match-recap-portland-timbers-2-san-jose-earthquakes-0]

|team2 = San Jose Earthquakes (1-MLS)

|goals1 = Asprilla {{goal|34}}
McInerney {{goal|40}}

|goals2 =

|stadium = Providence Park

|location = Portland, Oregon

|attendance = 16,834

|referee = Allen Chapman (Utah)

|stack = yes

}}

{{Football box collapsible

|date = June 14

|time = 7:30 PM PDT

|team1 = LA Galaxy (1-MLS)

|score = 4–1

|aet = yes

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-14-la-galaxy-vs-la-maquina-fc/boxscore Report]

|team2 = La Máquina FC (5-USASA)

|goals1 = Villarreal {{goal|15}}
Romney {{goal|96}}
Lletget {{goal|105|pen.|115}}

|goals2 = Borboa {{goal|28}}

|stadium = StubHub Center

|location = Carson, California

|attendance = 4,752

|referee = Ramon Hernandez (California)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:00 PM EDT

|team1 = Jacksonville Armada FC (2-NASL)

|score = 0–1

|report = [http://www.orlandocitysc.com/post/2016/06/16/orlando-city-advances-us-open-cup-round-16-1-0-win-over-jacksonville-armada-fc Report]

|team2 = Orlando City SC (1-MLS)

|goals1 =

|goals2 = Mateos {{goal|62}}

|stadium = Southern Oak Stadium

|location = Jacksonville, Florida

|attendance = 2,155

|referee = Rubiel Vazquez (Florida)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:00 PM EDT

|team1 = Philadelphia Union (1-MLS)

|score = 3–2

|report = [http://www.philadelphiaunion.com/post/2016/06/15/philadelphia-advances-round-16-us-open-cup-thanks-albergs-brace Report]

|team2 = Harrisburg City Islanders (3-USL)

|goals1 = Restrepo {{goal|3}}
Alberg {{goal|45+1||90+4}}

|goals2 = Foster {{goal|79}}
Warshaw {{goal|90+1}}

|stadium = Talen Energy Stadium

|location = Chester, Pennsylvania

|attendance =

|referee = Sorin Stoica (Ohio)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:00 PM EDT

|team1 = Columbus Crew SC (1-MLS)

|score = 4–0

|report = [http://www.columbuscrewsc.com/post/2016/06/16/four-goals-lead-crew-sc-us-open-cup-round-16 Report]

|team2 = Tampa Bay Rowdies (2-NASL)

|goals1 = Meram {{goal|41}}
Martínez {{goal|48}}
Finlay {{goal|52|pen.}}
Hollingsworth {{goal|65}}

|goals2 =

|stadium = Jesse Owens Memorial Stadium

|location = Columbus, Ohio

|attendance = 1,064

|referee = Greg Dopka (Illinois)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:05 PM EDT

|team1 = Rochester Rhinos (3-USL)

|score = 0–1

|report = [https://www.mlssoccer.com/matchcenter/rochester-rhinos-vsrbny-06-15-2016/]

|team2 = New York Red Bulls (1-MLS)

|goals1 =

|goals2 = Kljestan {{goal|20|pen.}}

|stadium = Rochester Rhinos Stadium

|location = Rochester, New York

|attendance = 3,596

|referee = Charles Murphy (Pennsylvania)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM EDT

|team1 = New York City FC (1-MLS)

|score = 0–1

|report = [http://www.nycfc.com/post/2016/06/15/new-york-city-fc-vs-new-york-cosmos-match-recap Report] [https://www.espn.com/soccer/match?gameId=449349 Report]

|team2 = New York Cosmos (2-NASL)

|goals1 =

|goals2 = Szetela {{goal|88}}

|stadium = Coffey Field

|location = New York, New York

|attendance = 4,500

|referee = Jorge Gonzalez (New York)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM EDT

|team1 = Carolina RailHawks (2-NASL)

|score = 0–1

|report = [http://www.revolutionsoccer.net/post/2016/06/16/revolution-defeat-railhawks-1-0-advance-round-16-usoc Report]

|team2 = New England Revolution (1-MLS)

|goals1 =

|goals2 = Herivaux {{goal|103}}

|stadium = WakeMed Soccer Park

|location = Cary, North Carolina

|attendance = 4,147

|referee = Kevin Terry (Texas)

|aet = yes

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM EDT

|team1 = D.C. United (1-MLS)

|score = 0–0

|report = [https://www.espn.com/soccer/match?gameId=449347]

|team2 = Fort Lauderdale Strikers (2-NASL)

|goals1 =

|goals2 =

|stadium = Maryland Soccerplex

|location = Boyds, Maryland

|attendance = 1,800

|referee = Matthew Kreitzer (Maryland)

|aet = yes

|penaltyscore = 3–4

|penalties1 = Büscher {{pengoal}}
Vincent {{pengoal}}
Kemp {{pengoal}}
Acosta {{penmiss}}
Jeffrey {{penmiss}}

|penalties2 = {{pengoal}} Victor
{{penmiss}} Maicon Santos
{{pengoal}} Gabriel
{{pengoal}} Núñez
{{pengoal}} Matheus Carvalho

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:00 PM CDT

|team1 = Minnesota United FC (2-NASL)

|score = 1–2

|report = [http://www.sportingkc.com/post/2016/06/15/recap-diego-rubio-stunner-gives-sporting-kc-2-1-win-lamar-hunt-us-open-cup Report]

|team2 = Sporting Kansas City (1-MLS)

|goals1 = Ramirez {{goal|82|pen.}}

|goals2 = Feilhaber {{goal|65|pen.}}
Rubio {{goal|109}}

|stadium = National Sports Center

|location = Blaine, Minnesota

|attendance = 7,689

|referee = Nima Saghafi (Oklahoma)

|aet = yes

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:15 PM CDT

|team1 = Houston Dynamo (1-MLS)

|score = 4–0

|report = [http://www.houstondynamo.com/post/2016/06/15/us-open-cup-recap-houston-dynamo-4-san-antonio-fc-0 Report]

|team2 = San Antonio FC (3-USL)

|goals1 = Manotas {{goal|39}}
Alex {{goal|45+5}}
Rodríguez {{goal|60}}
Miranda {{goal|66}}

|goals2 =

|stadium = BBVA Compass Stadium

|location = Houston, Texas

|attendance = 5,807

|referee = Leszek Stalmach (Texas)

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM CDT

|team1 = Chicago Fire (1-MLS)

|score = 1–1

|report = [http://www.chicago-fire.com/post/2016/06/15/chicago-fire-advance-past-indy-eleven-103rd-lamar-hunt-us-open-cup-pk-shootout Report]

|team2 = Indy Eleven (2-NASL)

|goals1 = Accam {{goal|111}}

|goals2 = Braun {{goal|105}}

|penalties1 = Igboananike {{penmiss}}
Thiam {{pengoal}}
Vincent {{pengoal}}
LaBrocca {{pengoal}}
Accam {{pengoal}}

|penalties2 = Paterson {{pengoal}}
Zayed {{pengoal}}
Vuković {{penmiss}}
Keller {{penmiss}}
Ubiparipović {{pengoal}}

|stadium = Toyota Park

|location = Bridgeview, Illinois

|attendance = 5,543

|referee = Elvis Mahmutovic (Missouri)

|aet = yes

|penaltyscore = 4–3

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM CDT

|team1 = FC Dallas (1-MLS)

|score = 2–2

|report = [http://www.fcdallas.com/post/2016/06/16/recap-fc-dallas-2-okc-energy-fc-2-fcd-advances-pks-6-5 Report]

|team2 = Oklahoma City Energy FC (3-USL)

|goals1 = Lizarazo {{goal|37||43}}

|goals2 = Rideout {{goal|26}}
Thomas {{goal|90}}

|penalties1 = Lizarazo {{pengoal}}
Acosta {{pengoal}}
Díaz {{pengoal}}
Hedges {{penmiss}}
Harris {{pengoal}}
Ortiz {{pengoal}}
Bonner {{pengoal}}

|penalties2 = König {{pengoal}}
Cuevas {{pengoal}}
Thomas {{pengoal}}
Wojcik {{penmiss}}
Tóth {{pengoal}}
Andrews {{pengoal}}
Hedrick {{penmiss}}

|stadium = Westcott Field

|location = Dallas, Texas

|attendance =

|referee = Guido Gonzales Jr. (Georgia)

|aet = yes

|penaltyscore = 6–5

|stack = yes

}}

{{Football box collapsible

|date = June 15

|time = 7:30 PM PDT

|team1 = Seattle Sounders FC (1-MLS)

|score = 2–0

|report = [http://www.soundersfc.com/post/2016/06/15/seattle-sounders-2-kitsap-pumas-0-cristian-roldan-and-joevin-jones-score-sounders Report]

|team2 = Kitsap Pumas (4-PDL)

|goals1 = Roldan {{goal|71}}
Jones {{goal|90+1}}

|goals2 =

|stadium = Starfire Sports Complex

|location = Tukwila, Washington

|attendance = 3,515

|referee = Chipalo Street (Washington)

|stack =

}}

Draw announced June 16. The fourth round winners were drawn into four 4-team brackets based on geographic considerations.

{{Football box collapsible

|date = June 28

|time = 7:30 PM CDT

|team1 = Chicago Fire (1-MLS)

|score = 2–1

|report = [http://www.chicago-fire.com/post/2016/06/28/chicago-fire-advance-us-open-cup-quarterfinal-2-1-win-over-columbus-crew-sc Report]

|team2 = Columbus Crew SC (1-MLS)

|goals1 = Accam {{goal|7||29}}

|goals2 = Finlay {{goal|79|pen.}}

|stadium = Toyota Park

|location = Bridgeview, Illinois

|attendance = 6,190

|referee = Nima Saghafi (Oklahoma)

|stack = yes

}}

{{Football box collapsible

|date = June 28

|time = 8:00 PM MDT

|team1 = Real Salt Lake (1-MLS)

|score = 1–1

|report = [http://www.rsl.com/post/2016/06/28/real-salt-lake-bows-out-us-open-cup-shootout-against-seattle Report]

|team2 = Seattle Sounders FC (1-MLS)

|goals1 = Plata {{goal|43|pen.}}

|goals2 = Valdez {{goal|51}}

|penalties1 = Martínez {{pengoal}}
Plata {{penmiss}}
Beckerman {{penmiss}}

|penalties2 = Gomez {{pengoal}}
Morris {{pengoal}}
Scott {{pengoal}}
Friberg {{pengoal}}

|stadium = Rio Tinto Stadium

|location = Sandy, Utah

|attendance = 17,087

|referee = Alex Chilowicz (New Jersey)

|aet = yes

|penaltyscore = 1–4

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 7:00 PM EDT

|team1 = Philadelphia Union (1-MLS)

|score = 2–1

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-29-philadelphia-union-vs-new-york-red-bulls Report]

|team2 = New York Red Bulls (1-MLS)

|goals1 = Pontius {{goal|55||60}}

|goals2 = Grella {{goal|17}}

|stadium = Talen Energy Stadium

|location = Chester, Pennsylvania

|attendance =

|referee = Mark Kadlecik (North Carolina)

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 6:00 PM CDT

|team1 = Houston Dynamo (1-MLS)

|score = 3–1

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-29-houston-dynamo-vs-sporting-kansas-city Report]

|team2 = Sporting Kansas City (1-MLS)

|goals1 = Manotas {{goal|7||84}}
Alex {{goal|55}}

|goals2 = Davis {{goal|61}}

|stadium = BBVA Compass Stadium

|location = Houston, Texas

|attendance = 6,371

|referee = Allen Chapman (Utah)

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 7:30 PM EDT

|team1 = New York Cosmos (2-NASL)

|score = 2–3

|report = [https://www.espn.com/soccer/match?gameId=452260]

|team2 = New England Revolution (1-MLS)

|goals1 = Bover {{goal|38}}
Guenzatti {{goal|56}}

|goals2 = Bunbury {{goal|43||83}}
Kamara {{goal|75}}

|stadium = Belson Stadium

|location = Jamaica, New York

|attendance = 1,264

|referee = Sorin Stoica (Ohio)

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 7:30 PM EDT

|team1 = Orlando City SC (1-MLS)

|score = 1–2

|aet = yes

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-29-orlando-city-sc-vs-fort-lauderdale-strikers Report]

|team2 = Fort Lauderdale Strikers (2-NASL)

|goals1 = Molino {{goal|15}}

|goals2 = Angulo {{goal|12}}
PC {{goal|120}}

|stadium = Camping World Stadium

|location = Orlando, Florida

|attendance = 3,162

|referee = Caleb Mendez (Florida)

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 7:30 PM CDT

|team1 = FC Dallas (1-MLS)

|score = 2–1

|aet = yes

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-29-fc-dallas-vs-colorado-rapids Report]

|team2 = Colorado Rapids (1-MLS)

|goals1 = Díaz {{goal|64}}
Urruti {{goal|96}}

|goals2 = Badji {{goal|38}}

|stadium = Toyota Stadium

|location = Frisco, Texas

|attendance =

|referee = Ismail Elfath (Texas)

|stack = yes

}}

{{Football box collapsible

|date = June 29

|time = 7:30 PM PDT

|team1 = Portland Timbers (1-MLS)

|score = 0–1

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-06-29-portland-timbers-vs-la-galaxy Report]

|team2 = LA Galaxy (1-MLS)

|goals1 =

|goals2 = Mendiola {{goal|5}}

|stadium = Providence Park

|location = Portland, Oregon

|attendance = 18,164

|referee = Edvin Jurisevic (Nebraska)

|stack = yes

}}

The eight round of 16 winners played the other survivor in their geographic arm of the bracket.

{{Football box collapsible

|date = July 20

|time = 7:30 PM EDT

|team1 = New England Revolution (1-MLS)

|score = 1–1

|report = https://www.espn.com/soccer/match?gameId=457290

|team2 = Philadelphia Union (1-MLS)

|goals1 = Watson {{goal|44}}

|goals2 = Herbers {{goal|90}}

|penalties1 = Nguyen {{pengoal}}
Tierney {{pengoal}}
Caldwell {{penmiss}}
Gonçalves {{pengoal}}
Watson {{pengoal}}

|penalties2 = {{penmiss}} Le Toux
{{pengoal}} Ilsinho
{{penmiss}} Sapong
{{pengoal}} Herbers

|stadium = Jordan Field

|location = Boston, Massachusetts

|attendance = 4,000

|referee = Sorin Stoica (Ohio)

|aet = yes

|penaltyscore = 4–2

|stack = yes

}}

{{Football box collapsible

|date = July 20

|time = 7:30 PM CDT

|team1 = Chicago Fire (1-MLS)

|score = 3–0

|report =

|team2 = Fort Lauderdale Strikers (2-NASL)

|goals1 = Accam {{goal|5}}
de Leeuw {{goal|35}}
Thiam {{goal|51}}

|goals2 =

|stadium = Toyota Park

|location = Bridgeview, Illinois

|attendance = 3,666

|referee = Fotis Bazakos (Minnesota)

|stack = yes

}}

{{Football box collapsible

|date = July 20

|time = 7:30 PM CDT

|team1 = Houston Dynamo (1-MLS)

|score = 0–1

|report =

|team2 = FC Dallas (1-MLS)

|goals1 =

|goals2 = Castillo {{goal|90+1}}

|stadium = BBVA Compass Stadium

|location = Houston, Texas

|attendance =10,150

|referee = Nima Saghafi (Oklahoma)

|stack = yes

}}

{{Football box collapsible

|date = July 20

|time = 7:30 PM PDT

|team1 = LA Galaxy (1-MLS)

|score = 4–2

|report =

|team2 = Seattle Sounders FC (1-MLS)

|goals1 = Gordon {{goal|17}}
dos Santos {{goal|77}}
Lletget {{goal|85||88}}

|goals2 = Farfan {{goal|4}}
Gómez {{goal|58}}

|stadium = StubHub Center

|location = Carson, California

|attendance = 3,409

|referee = Alex Chilowicz (New Jersey)

|stack =

}}

The draw to determine the semifinal hosts for the final teams from the eastern and western halves of the bracket took place on July 21 at Soccer House in Chicago and was conducted by U.S. Open Cup Commissioner Paul Marstaller. The semifinal matches were held on August 9–10.{{cite web|url=http://www.ussoccer.com/stories/2016/07/21/17/45/160721-usoc-new-england-revolution-la-galaxy-host-2016-us-open-cup-semifinals|title=New England Revolution, LA Galaxy to Host 2016 U.S. Open Cup Semifinals|website=www.ussoccer.com|access-date=2016-07-22}}

{{Football box collapsible

|date = August 9

|time = 8:00 PM EDT

|team1 = New England Revolution (1-MLS)

|score = 3–1

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-08-09-new-england-revolution-vs-chicago-fire/feed Report]

|team2 = Chicago Fire (1-MLS)

|goals1 = Kamara {{goal|16|pen.}}
Watson {{goal|42}}
Bunbury {{goal|85}}

|goals2 = Accam {{goal|40}}

|stadium = Gillette Stadium

|location = Foxborough, Massachusetts

|attendance = 9,801

|referee = Edvin Jurisevic (Nebraska)

|stack = yes

}}

{{Football box collapsible

|date = August 10

|time = 7:00 PM PDT

|team1 = LA Galaxy (1-MLS)

|score = 1–2

|aet = yes

|report = [http://www.lagalaxy.com/post/2016/08/10/match-report-la-galaxy-drop-open-cup-semifinal-heartbreaker-fc-dallas-2-1 Report]

|team2 = FC Dallas (1-MLS)

|goals1 = Magee {{goal|101|pen.}}

|goals2 = Hedges {{goal|116}}
Ulloa {{goal|120+1}}

|stadium = StubHub Center

|location = Carson, California

|attendance = 6,120

|referee = Ted Unkel (Florida)

|stack = yes

}}

{{main|2016 U.S. Open Cup Final}}

The hosting priority for the 2016 U.S. Open Cup Final took place subsequent to the semifinal host draw. The order went as follows: Chicago Fire, LA Galaxy, FC Dallas, New England Revolution.

{{Football box collapsible

|date = September 13

|time = 9:00 PM CDT

|team1 = FC Dallas (1-MLS)

|score = 4–2

|report = [http://matchcenter.mlssoccer.com/matchcenter/2016-09-13-fc-dallas-vs-new-england-revolution Report]

|team2 = New England Revolution (1-MLS)

|goals1 = Urruti {{goal|15||61}}
Hedges {{goal|40}}
Díaz {{goal|45+7|pen.}}
Zimmerman {{yel|51}}

|goals2 = Agudelo {{goal|6||73}}
Caldwell {{yel|29}}
Woodberry {{yel|48}}
Farrell {{yel|63}}
Fagundez {{yel|66}}

|stadium = Toyota Stadium

|location = Frisco, Texas

|attendance = 16,612

|referee = Baldomero Toledo (California)

}}

Players and teams still active in bold.{{cite web|last1=Hakala|first1=Josh|title=2016 Lamar Hunt US Open Cup statistical leaders: Goals, assists, points|url=http://thecup.us/2016/06/13/2016-lamar-hunt-us-open-cup-statistical-leaders-goals-assists-points/|website=thecup.us|access-date=16 June 2016}}

{{reflist|30em}}

• [http://thecup.us/ The Cup.us – Full Coverage of the US Open Cup]

{{US Open Cup}}

{{2016 in American soccer}}

{{2015–16 in North and Central America, and Caribbean football (CONCACAF)}}

Category:U.S. Open Cup

U.S. Open Cup