2023 U.S. Open Cup

{{main|2023 U.S. Open Cup qualification}}

Professional teams that are majority-owned or controlled by higher-division professional clubs are barred from entering the competition. On March 10, 2023, Rochester New York FC announced they would not compete in the 2023 MLS Next Pro season, and were in fact ceasing operations.{{cite web |url=https://www.mlsnextpro.com/news/update-on-rochester-new-york-fc-and-mls-next-pro |title=Update on Rochester New York FC and MLS NEXT Pro |date=March 10, 2023 |website=MLSNextPro.com |publisher=MLS Next Pro |access-date=March 10, 2023}}

• Bold denotes team is still active in the tournament.
• {{anchor|Money}} $: Winner of $25,000 bonus for advancing the furthest in the competition from their respective divisions.
• {{anchor|Runner-up}} $$: Winner of $100,000 for being the runner-up in the competition.
• {{anchor|Champion}} $$$: Winner of $300,000 for winning the competition.{{cite web |url=https://www.npsl.com/usoc-2023/ |title=About the U.S. Open Cup |website=NPSL.com |publisher=National Premier Soccer League |access-date=November 20, 2022}}

{{notelist}}

{{refbegin}}

{{reflist|group=lower-roman}}

{{refend}}

{{Location map+ |USA |width=1300 |float=center |caption=Locations of teams in the tournament proper.

{{hlist

| 8px MLS

| 8px USLC

| 8px Division 3 (NISA/USL1)

| 8px Open Division (NPSL/USL2)

| 8px Local Qualifiers & National Amateur Cup Champion

| 8px Multiple Teams

}}

Metro areas with multiple teams:

• Charlotte: Charlotte FC (MLS), Charlotte Independence (USL1)
• Chattanooga: Chattanooga FC (NISA), Chattanooga Red Wolves SC (USL1)
• Chicago: Chicago Fire FC (MLS), Chicago House AC (Local)
• Detroit: Detroit City FC (USLC), Michigan Stars FC (NISA), Gold Star FC Detroit (NISA)
• Hartford: Hartford Athletic (USLC), Hartford City FC (NPSL)
• Los Angeles: Los Angeles FC (MLS), LA Galaxy (MLS), Orange County SC (USLC), Los Angeles Force (NISA)
• Miami: Inter Miami CF (MLS), Miami FC (USLC), Miami United FC (Local)
• Nashville: Nashville SC (MLS), Beaman United FC (Local)
• New York City: New York City FC (MLS), New York Red Bulls (MLS), Manhattan SC (USL2), FC Motown (NPSL), Lansdowne Yonkers FC (Local)
• Orlando: Orlando City SC (MLS), Club de Lyon (NISA), Nona FC (USL2), Club de Lyon 2 (Local)
• Philadelphia: Philadelphia Union (MLS), West Chester United SC (USL2)
• San Diego: San Diego Loyal SC (USLC), Albion San Diego (NISA)
• San Francisco Bay Area: San Jose Earthquakes (MLS), Oakland Roots SC (USLC), Project 51O (USL2), El Farolito SC (NPSL), Inter San Francisco (Local)
• Seattle: Seattle Sounders FC (MLS), Crossfire Redmond (NPSL)
• Tulsa: FC Tulsa (USLC), Tulsa Athletic (NPSL)
• Washington D.C.: D.C. United (MLS), Loudoun United FC (USLC)

|places=

{{Location map~ |USA |lat=33.74899537073518 |long=-84.3879717810452 |label=Atlanta United FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=30.387742934571232 |long=-97.71938014636318 |label=Austin FC |label_size=80 |position=left |mark=Red pog.svg}}

{{Location map~ |USA |lat=39.11103328098898 |long=-84.52285872056366 |label=FC Cincinnati |label_size=80 |position=top |mark=Red pog.svg}}

{{Location map~ |USA |lat=39.73933852440657 |long=-104.99016710747496 |label=Colorado Rapids |label_size=80 |position=left |mark=Red pog.svg}}

{{Location map~ |USA |lat=39.968451656675654 |long=-83.01709494651429 |label=Columbus Crew |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=33.154444 |long=-96.835278 |label=FC Dallas |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=29.752255671778688 |long=-95.35222014719191 |label=Houston Dynamo FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=44.97774956227467 |long=-93.2649897680113 |label=Minnesota United FC |label_size=80 |position=top |mark=Red pog.svg}}

{{Location map~ |USA |lat=42.091 |long=-71.264 |label=New England Revolution|label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=45.51517179226837 |long=-122.67834753903254 |label=Portland Timbers |label_size=80 |position=top |mark=Red pog.svg}}

{{Location map~ |USA |lat=40.582929184372404 |long=-111.8933594914704 |label=Real Salt Lake |label_size=80 |position=left |mark=Red pog.svg}}

{{Location map~ |USA |lat=39.12163118064744 |long=-94.8231994975958 |label=Sporting Kansas City |label_size=80 |position=left |mark=Red pog.svg}}

{{Location map~ |USA |lat=38.62692745995879 |long=-90.19915023580369 |label=St. Louis City SC |label_size=80 |position=bottom |mark=Red pog.svg}}

{{Location map~ |USA |lat=33.51860982216501 |long=-86.81046673158751 |label=Birmingham Legion FC |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=32.77649521763172 |long=-79.9309881312091 |label=Charleston Battery |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.83390079067579 |long=-104.82150063775298 |label=Colorado Springs Switchbacks FC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=31.761954479179543 |long=-106.48492429902919 |label=El Paso Locomotive FC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=39.7684897971886 |long=-86.15803260219954 |label=Indy Eleven |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=36.16922858479163 |long=-115.14065221352614 |label=Las Vegas Lights FC |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.252709073571815 |long=-85.75850187080235 |label=Louisville City FC |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=35.14951896545076 |long=-90.04905223697526 |label=Memphis 901 FC |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=36.61490418002709 |long=-121.82204912721032 |label=Monterey Bay FC |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=35.08418344509937 |long=-106.65086953523182 |label=New Mexico United |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=33.448357911068946 |long=-112.07415273142104 |label=Phoenix Rising FC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=40.44066553092845 |long=-79.99595433437983 |label=Pittsburgh Riverhounds SC |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=26.301726364795066 |long=-98.16334476174318 |label=Rio Grande Valley FC Toros |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.58173860292029 |long=-121.4945425441788 |label=Sacramento Republic FC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=29.424126971749406 |long=-98.49371772852207 |label=San Antonio FC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=27.76760384836234 |long=-82.64016609082519 |label=Tampa Bay Rowdies |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=29.218103 |long=-81.031723 |label=Club de Lyon FC |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=43.156618577280796 |long=-77.60884811885286 |label=City Union |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=36.73782216056503 |long=-119.7871543150927 |label=Central Valley Fuego FC |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=43.07305651659342 |long=-89.40122671893994 |label=Forward Madison FC |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=34.85263694973682 |long=-82.39409386584747 |label=Greenville Triumph SC |label_size=80 |position=bottom |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=38.029722 |long=-84.494722 |label=Lexington SC |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=39.18501420930216 |long=-77.3106495526165 |label=Maryland Bobcats FC |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=35.79143485820475 |long=-78.78113885194223 |label=North Carolina FC |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=40.477479786508205 |long=-104.90135727746456 |label=Northern Colorado Hailstorm FC |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=35.9617 |long=-83.9232 |label=One Knoxville SC |label_size=80 |position=bottom |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=37.54069231180277 |long=-77.4360245445324 |label=Richmond Kickers |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=32.081111 |long=-81.091111 |label=Savannah Clovers FC |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=32.44881401911466 |long=-81.78325284439606 |label=South Georgia Tormenta FC |label_size=80 |position=bottom |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=41.2565273110203 |long=-95.93449814923649 |label=Union Omaha |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=36.211389 |long=-81.668611 |label=Appalachian FC |label_size=80 |position=left |mark=Green pog.svg}}

{{Location map~ |USA |lat=30.6452 |long=-96.3696 |label=Brazos Valley Cavalry FC |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=41.499263207713454 |long=-81.69458163384819 |label=Cleveland SC |label_size=80 |position=left |mark=Green pog.svg}}

{{Location map~ |USA |lat=41.58681948015526 |long=-93.62491357165688 |label=Des Moines Menace |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=30.336944 |long=-81.661389 |label=Jacksonville Armada U-23 |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=37.070833 |long=-76.484444 |label=Lionsbridge FC |label_size=80 |position=bottom |mark=Green pog.svg}}

{{Location map~ |USA |lat=36.21271615454268 |long=-79.71326524106982 |label=NC Fusion |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=39.27763593037902 |long=-74.57471625452628 |label=Ocean City Nor'easters |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=40.646066141574664 |long=-111.49798428929655 |label=Park City Red Wolves SC |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=34.2776 |long=-119.2317 |label=Ventura County Fusion |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=33.499444 |long=-117.661667 |label=Capo FC |label_size=80 |position=right |mark=Blue pog.svg}}

{{Location map~ |USA |lat=43.13 |long=-87.927778 |label=Bavarian United SC |label_size=80 |position=top |mark=Blue pog.svg}}

{{Location map~ |USA |lat=32.314444 |long=-106.778889 |label=UDA Soccer |label_size=80 |position=top |mark=Blue pog.svg}}

{{Location map~ |USA |lat=35.22721552837581 |long=-80.84309227188619 |label=Charlotte |label_size=80 |position=top |mark=Purple pog.svg}}

{{Location map~ |USA |lat=35.045719432756805 |long=-85.31043593018825 |label=Chattanooga |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=41.87816478970271 |long=-87.62985944017429 |label=Chicago |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=42.331506643194736 |long=-83.04569005672376 |label=Detroit |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=41.76584042503873 |long=-72.67333170291519 |label=Hartford |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=34.052245327498575 |long=-118.24349853174114 |label=Los Angeles |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=25.761707708487393 |long=-80.1919419163021 |label=Miami |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=36.16646779431836 |long=-86.77128726417052 |label=Nashville |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=39.95260886864184 |long=-75.16521510925807 |label=Philadelphia |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=40.71280149342799 |long=-74.00597513865641 |label=NYC |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=28.53838221973686 |long=-81.3789598327052 |label=Orlando |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=32.715718954604114 |long=-117.1611666674325 |label=San Diego |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=37.77499238831668 |long=-122.41921349305606 |label=San Francisco Bay Area |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=47.59513326556388 |long=-122.33164865965206 |label=Seattle |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=36.15399014678964 |long=-95.99281508409018 |label=Tulsa |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=38.907305224249235 |long=-77.0365143863283 |label=Washington D.C.|label_size=80 |position=right |mark=Purple pog.svg}}

}}

A total of 34 states and the District of Columbia are represented by clubs in the U.S. Open Cup this year.

States without a team in the Open Cup: Alaska, Arkansas, Delaware, Hawaii, Idaho, Louisiana, Maine, Mississippi, Montana, New Hampshire, North Dakota, Rhode Island, South Dakota, Vermont, West Virginia, and Wyoming.

21 teams made their Open Cup debuts in the 2023 tournament.

• MLS: St. Louis City SC
• USLC: Loudoun United FC
• NISA: Club de Lyon, Gold Star FC Detroit, Savannah Clovers FC
• USL1: Lexington SC, One Knoxville SC
• NPSL: Appalachian FC, Crossfire Redmond, Jacksonville Armada U-23
• USL2: Lionsbridge FC, Manhattan SC, Nona FC, Project 51O
• UPSL: Beaman United FC, UDA Soccer
• Other Open Division: Capo FC, Chicago House AC, Club de Lyon 2, Inter San Francisco, West Chester United SC

The complete draw for the First round, including match dates and times, was announced on February 3, 2023.{{cite press release |date=February 3, 2023 |title=First round of 100-Team 2023 Lamar Hunt U.S. Open Cup Kicks off with 14 Games on March 21–23 |url=https://www.ussoccer.com/stories/2023/02/first-round-of-100-team-2023-lamar-hunt-us-open-cup-kicks-off-with-14-games-on-march-21-23 |publisher=United States Soccer Federation |access-date=February 4, 2023}}

The draw for the Second round pairings and hosting scenarios was announced on February 16, 2023.{{cite press release|date=February 16, 2023 |title=Second round Pairings and Host Scenarios Set for 2023 Lamar Hunt U.S. Open Cup|url=https://www.ussoccer.com/stories/2023/02/second-round-pairings-and-host-scenarios-set-for-2023-lamar-hunt-us-open-cup|publisher=United States Soccer Federation|access-date=February 16, 2023}}

{{2NTeam-NTeamBracket

| rounds = 2

| brackets = 30

| RD1-byes = y

| boldwinner = y

| seed-width = 50

| team-width = 205

| score-width = 30

| RD1 = First round
March 21–23

| RD2 = Second round
April 4–11

| RD1-seed01 = NPSL

| RD1-team01 = El Farolito SC {{aet}}

| RD1-score01 = 3

| RD1-seed02 = LQ

| RD1-team02 = Inter San Francisco

| RD1-score02 = 0

| RD1-seed05 = LQ

| RD1-team05 = West Chester United SC

| RD1-score05 = 1

| RD1-seed06 = USL2

| RD1-team06 = Ocean City Nor'easters

| RD1-score06 = 3

| RD1-seed09 = LQ

| RD1-team09 = Club de Lyon 2

| RD1-score09 = 0

| RD1-seed10 = USL2

| RD1-team10 = Nona FC

| RD1-score10 = 1

| RD1-seed13 = NPSL

| RD1-team13 = Appalachian FC {{pso}}

| RD1-score13 = 2 (3)

| RD1-seed14 = USL2

| RD1-team14 = North Carolina Fusion U23

| RD1-score14 = 2 (0)

| RD1-seed19 = USL2

| RD1-team19 = Manhattan SC {{aet}}

| RD1-score19 = 2

| RD1-seed20 = NPSL

| RD1-team20 = FC Motown

| RD1-score20 = 1

| RD1-seed23 = LQ

| RD1-team23 = Chicago House AC

| RD1-score23 = 1

| RD1-seed24 = NAC

| RD1-team24 = Bavarian United SC

| RD1-score24 = 0

| RD1-seed27 = LQ

| RD1-team27 = Beaman United FC

| RD1-score27 = 0

| RD1-seed28 = USL2

| RD1-team28 = Des Moines Menace

| RD1-score28 = 3

| RD1-seed31= NPSL

| RD1-team31 = Tulsa Athletic

| RD1-score31 = 1

| RD1-seed32 = USL2

| RD1-team32 = Brazos Valley Cavalry FC

| RD1-score32 = 0

| RD1-seed37 = LQ

| RD1-team37 = UDA Soccer

| RD1-score37 = 1

| RD1-seed38 = USL2

| RD1-team38 = Park City Red Wolves SC

| RD1-score38 = 0

| RD1-seed41 = USL2

| RD1-team41 = Project 51O

| RD1-score41 = 0

| RD1-seed42 = NPSL

| RD1-team42 = Crossfire Redmond

| RD1-score42 = 3

| RD1-seed45 = NPSL

| RD1-team45 = Cleveland SC

| RD1-score45 = 1

| RD1-seed46 = USL2

| RD1-team46 = Lionsbridge FC

| RD1-score46 = 0

| RD1-seed51 = NPSL

| RD1-team51 = Jacksonville Armada U-23 {{pso}}

| RD1-score51 = 1 (9)

| RD1-seed52 = LQ

| RD1-team52 = Miami United FC

| RD1-score52 = 1 (8)

| RD1-seed55 = NPSL

| RD1-team55 = Hartford City FC

| RD1-score55 = 1

| RD1-seed56 = LQ

| RD1-team56 = Lansdowne Yonkers FC {{aet}}

| RD1-score56 = 2

| RD1-seed59 = USL2

| RD1-team59 = Ventura County Fusion

| RD1-score59 = 1

| RD1-seed60 = LQ

| RD1-team60 = Capo FC

| RD1-score60 = 4

| RD2-seed01 = USLC

| RD2-team01 = Oakland Roots SC

| RD2-score01 = 3

| RD2-seed02 = NPSL

| RD2-team02 = El Farolito SC

| RD2-score02 = 1

| RD2-seed03 = USL1

| RD2-team03 = Union Omaha

| RD2-score03 = 2

| RD2-seed04 = USLC

| RD2-team04 = El Paso Locomotive FC

| RD2-score04 = 0

| RD2-seed05 = NISA

| RD2-team05 = Maryland Bobcats FC {{aet}}

| RD2-score05 = 3

| RD2-seed06 = USL2

| RD2-team06 = Ocean City Nor'easters

| RD2-score06 = 2

| RD2-seed07 = USL1

| RD2-team07 = Central Valley Fuego FC

| RD2-score07 = 1

| RD2-seed08 = USLC

| RD2-team08 = Monterey Bay FC {{aet}}

| RD2-score08 = 3

| RD2-seed09 = USLC

| RD2-team09 = Tampa Bay Rowdies

| RD2-score09 = 2

| RD2-seed10 = USL2

| RD2-team10 = Nona FC

| RD2-score10 = 0

| RD2-seed11 = USLC

| RD2-team11 = Detroit City FC

| RD2-score11 = 1

| RD2-seed12 = NISA

| RD2-team12 = Gold Star FC Detroit

| RD2-score12 = 0

| RD2-seed13 = USL1

| RD2-team13 = Charlotte Independence

| RD2-score13 = 2

| RD2-seed14 = NPSL

| RD2-team14 = Appalachian FC

| RD2-score14 = 0

| RD2-seed15 = USLC

| RD2-team15 = Indy Eleven {{aet}}

| RD2-score15 = 3

| RD2-seed16 = NISA

| RD2-team16 = Michigan Stars FC

| RD2-score16 = 1

| RD2-seed17 = USLC

| RD2-team17 = Louisville City FC

| RD2-score17 = 1

| RD2-seed18 = USL1

| RD2-team18 = Lexington SC

| RD2-score18 = 0

| RD2-seed19 = NISA

| RD2-team19 = Flower City Union

| RD2-score19 = 3

| RD2-seed20 = USL2

| RD2-team20 = Manhattan SC

| RD2-score20 = 1

| RD2-seed21 = USLC

| RD2-team21 = Loudoun United FC {{aet}}

| RD2-score21 = 2

| RD2-seed22 = USL1

| RD2-team22 = North Carolina FC

| RD2-score22 = 1

| RD2-seed23 = USL1

| RD2-team23 = Forward Madison FC

| RD2-score23 = 2

| RD2-seed24 = LQ

| RD2-team24 = Chicago House AC {{aet}}

| RD2-score24 = 3

| RD2-seed25 = USLC

| RD2-team25 = Pittsburgh Riverhounds SC

| RD2-score25 = w/o{{cite web |url=https://www.ussoccer.com/stories/2023/03/pittsburgh-riverhounds-through-to-open-cup-third-round-as-rochester-nyfc-ceases-operations |title=Pittsburgh Riverhounds Through to 2023 Open Cup Third Round via Forfeit as Rochester NY FC Ceases Operations |date=March 13, 2023 |website=USSoccer.com |publisher=United States Soccer Federation |access-date=March 13, 2023}}

| RD2-seed26 = MLSNP

| RD2-team26 = {{strikethrough|Rochester New York FC}}

| RD2-score26 =

| RD2-seed27 = USL2

| RD2-team27 = Des Moines Menace

| RD2-score27 = 1

| RD2-seed28 = NISA

| RD2-team28 = Chattanooga FC

| RD2-score28 = 4

| RD2-seed29 = USLC

| RD2-team29 = Charleston Battery

| RD2-score29 = 4

| RD2-seed30 = NISA

| RD2-team30 = Savannah Clovers FC

| RD2-score30 = 1

| RD2-seed31 = USLC

| RD2-team31 = FC Tulsa

| RD2-score31 = 0

| RD2-seed32 = NPSL

| RD2-team32 = Tulsa Athletic

| RD2-score32 = 1

| RD2-seed33 = NISA

| RD2-team33 = Club de Lyon (D3)

| RD2-score33 = 1

| RD2-seed34 = USLC

| RD2-team34 = San Antonio FC {{aet}}

| RD2-score34 = 2

| RD2-seed35 = USL1

| RD2-team35 = One Knoxville SC

| RD2-score35 = 1

| RD2-seed36 = USLC

| RD2-team36 = Memphis 901 FC {{aet}}

| RD2-score36 = 2

| RD2-seed37 = USLC

| RD2-team37 = New Mexico United

| RD2-score37 = 6

| RD2-seed38 = LQ

| RD2-team38 = UDA Soccer

| RD2-score38 = 0

| RD2-seed39 = USL1

| RD2-team39 = Chattanooga Red Wolves SC

| RD2-score39 = 1

| RD2-seed40 = USLC

| RD2-team40 = Birmingham Legion FC

| RD2-score40 = 4

| RD2-seed41 = USLC

| RD2-team41 = Sacramento Republic FC {{aet}}

| RD2-score41 = 5

| RD2-seed42 = NPSL

| RD2-team42 = Crossfire Redmond

| RD2-score42 = 4

| RD2-seed43 = USL1

| RD2-team43 = South Georgia Tormenta FC

| RD2-score43 = 2

| RD2-seed44 = USLC

| RD2-team44 = Rio Grande Valley FC Toros

| RD2-score44 = 1

| RD2-seed45 = USL1

| RD2-team45 = Richmond Kickers

| RD2-score45 = 3

| RD2-seed46 = NPSL

| RD2-team46 = Cleveland SC

| RD2-score46 = 2

| RD2-seed47 = USLC

| RD2-team47 = Colorado Springs Switchbacks FC

| RD2-score47 = 1

| RD2-seed48 = USL1

| RD2-team48 = Northern Colorado Hailstorm FC {{aet}}

| RD2-score48 = 3

| RD2-seed49 = USLC

| RD2-team49 = Las Vegas Lights FC

| RD2-score49 = 4

| RD2-seed50 = NISA

| RD2-team50 = Los Angeles Force

| RD2-score50 = 0

| RD2-seed51 = USLC

| RD2-team51 = Miami FC

| RD2-score51 = 3

| RD2-seed52 = NPSL

| RD2-team52 = Jacksonville Armada U-23

| RD2-score52 = 1

| RD2-seed53 = USLC

| RD2-team53 = San Diego Loyal SC

| RD2-score53 = 2

| RD2-seed54 = NISA

| RD2-team54 = Albion San Diego

| RD2-score54 = 0

| RD2-seed55 = USLC

| RD2-team55 = Hartford Athletic

| RD2-score55 = 3

| RD2-seed56 = LQ

| RD2-team56 = Lansdowne Yonkers FC

| RD2-score56 = 0

| RD2-seed57 = USLC

| RD2-team57 = Phoenix Rising FC

| RD2-score57 = 1

| RD2-seed58 = USL1

| RD2-team58 = Greenville Triumph SC

| RD2-score58 = 0

| RD2-seed59 = USLC

| RD2-team59 = Orange County SC

| RD2-score59 = 5

| RD2-seed60 = LQ

| RD2-team60 = Capo FC

| RD2-score60 = 0

}}

Host team listed first

Bold = winner

(a.e.t.) = after extra time, (p) = penalty shootout score

All times local to game site.

{{Football box collapsible

|date = March 21

|time = 7:30 pm EDT

|team1 = West Chester United SC (LQ) {{flagicon|Pennsylvania}}

|score = 1–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/wcuvsocn-03-21-2023/

|team2 = {{flagicon|New Jersey}} Ocean City Nor'easters (USL2)

|goals1 =

• Reese {{goal|12}}

|goals2 =

• Latorre {{goal|18}}
• Evande {{goal|60}}
• Sabino {{goal|67|pen.}}

|stadium = YSC Sport

|location = Wayne, Pennsylvania

|attendance = 377

|referee = Joshua Encarnación

}}

{{Football box collapsible

|date = March 22

|time = 2:30 pm PDT

|team1 = El Farolito SC (NPSL) {{flagicon|California}}

|score = 3–0

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/farvsisf-03-22-2023

|team2 = {{flagicon|California}} International San Francisco SC (LQ)

|goals1 =

• Mosquera {{goal|94}}
• Quiñones {{goal|99}}
• Benitez {{goal|113}}

|goals2 =

|stadium = Ohlone College Soccer Stadium

|location = Fremont, California

|attendance = 200

|referee = Pedro Vasquez-Luna

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm EDT

|team1 = Club de Lyon 2 (LQ) {{flagicon|Florida}}

|score = 0–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cl2vsnfc-03-22-2023/

|team2 = {{flagicon|Florida}} Nona FC (USL2)

|goals1 =

|goals2 =

• Eyang {{goal|1}}

|stadium = Austin-Tindall Sports Complex

|location = Kissimmee, Florida

|attendance = 350

|referee = Alexandra Billeter

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm EDT

|team1 = Appalachian FC (NPSL) {{flagicon|North Carolina}}

|score = 2–2

|aet = yes

|penaltyscore = 3–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/afcvsncf-03-22-2023

|team2 = {{flagicon|North Carolina}} North Carolina Fusion U23 (USL2)

|goals1 =

• Holbrook {{goal|1||32}}

|goals2 =

• Fernandez {{goal|38}}
• Ferreira {{goal|79}}

|penalties1 =

• Landau {{pengoal}}
• Arguello {{pengoal}}
• de Lange {{pengoal}}

|penalties2 =

• {{penmiss}} Neijenhuis
• {{penmiss}} Leray
• {{penmiss}} Bethell

|stadium = Ted Mackorell Soccer Complex

|location = Boone, North Carolina

|attendance = 1,811

|referee = Emma Richards

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm EDT

|team1 = Manhattan SC (USL2) {{flagicon|New York}}

|score = 2–1

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/mscvsmtw-03-22-2023/

|team2 = {{flagicon|New Jersey}} FC Motown (NPSL)

|goals1 =

• Karamoko {{goal|55}}
• Lulka {{goal|106}}

|goals2 =

• Catania {{goal|26}}

|stadium = Marillac Field (College of Mt. St. Vincent)

|location = Bronx, New York

|attendance = 104

|referee = Christian Centeno

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm CDT

|team1 = Chicago House AC (LQ) {{flagicon|Illinois}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chivsbav-03-22-2023/

|team2 = {{flagicon|Wisconsin}} Bavarian United SC (NAC)

|goals1 =

• Makowiecki {{goal|39}}

|goals2 =

|stadium = Langhorst Field (Elmhurst University)

|location = Elmhurst, Illinois

|attendance = 310

|referee = Peter Bernardy

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm CDT

|team1 = Beaman United FC (LQ) {{flagicon|Tennessee}}

|score = 0–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/bufcvsdmm-03-22-2023/

|team2 = {{flagicon|Iowa}} Des Moines Menace (USL2)

|goals1 =

|goals2 =

• Rodriguez {{goal|21||57|pen.}}
• Garcia {{goal|90+5}}

|stadium = Dean A. Hayes Track & Soccer Stadium (MTSU)

|location = Murfreesboro, Tennessee

|attendance = 400

|referee = Benjamin Meyer

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm CDT

|team1 = Tulsa Athletic (NPSL) {{flagicon|Oklahoma}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/tatvsbrz-03-22-2023/

|team2 = {{flagicon|Texas}} Brazos Valley Cavalry FC (USL2)

|goals1 =

• Cvetanović {{goal|31}}

|goals2 =

|stadium = Hicks Park

|location = Tulsa, Oklahoma

|attendance = 400

|referee = Matthew Corrigan

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm MDT

|team1 = UDA Soccer (LQ) {{flagicon|New Mexico}}

|score = 1–0

|report = https://www.espn.com/soccer/match/_/gameId/664888

|team2 = {{flagicon|Utah}} Park City Red Wolves SC (USL2)

|goals1 = Puente {{goal|79|pen.}}

|goals2 =

|stadium = New Mexico State Soccer Field

|location = Las Cruces, New Mexico

|attendance = 439

|referee = Cristian Campo Hernández

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm PDT

|team1 = Project 51O (USL2) {{flagicon|California}}

|score = 0–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/provscfr-03-22-2023/

|team2 = {{flagicon|Washington}} Crossfire Redmond (NPSL)

|goals1 =

|goals2 =

• Hauswirth {{goal|49}}
• Howard {{goal|85}}
• Haddadi {{goal|90+5}}

|stadium = Ohlone College Soccer Field

|location = Fremont, California

|attendance = 280

|referee = Brian Cordova

}}

{{Football box collapsible

|date = March 22

|time = 7:00 pm PDT

|team1 = Ventura County Fusion (USL2) {{flagicon|California}}

|score = 1–4

|report = https://www.espn.com/soccer/match/_/gameId/664893

|team2 = {{flagicon|California}} Capo FC (LQ)

|goals1 =

• Desdunes {{goal|6}}

|goals2 =

• Gallardo {{goal|16}}
• Mota {{goal|45+3||77}}
• Scalzo {{goal|53}}

|stadium = Ventura College Sportscomplex

|location = Ventura, California

|attendance = 50

|referee = Irving Navarro

}}

{{Football box collapsible

|date = March 23

|time = 7:00 pm EDT

|team1 = Cleveland SC (NPSL) {{flagicon|Ohio}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/clsvslfc-03-23-2023/

|team2 = {{flagicon|Virginia}} Lionsbridge FC (USL2)

|goals1 =

• Cvecko {{goal|45+1}}

|goals2 =

|stadium = North Olmsted High School

|location = North Olmsted, Ohio

|attendance = 200

|referee = Kevin Fikar

}}

{{Football box collapsible

|date = March 23

|time = 7:00 pm EDT

|team1 = Jacksonville Armada U-23 (NPSL) {{flagicon|Florida}}

|score = 1–1

|aet = yes

|penaltyscore = 9–8

|report = https://www.espn.com/soccer/match?gameId=664890

|team2 = {{flagicon|Florida}} Miami United FC (LQ)

|goals1 =

• Thomas {{goal|43}}

|goals2 =

• Dominguez {{goal|49}}

|penalties1 =

• Johnson {{pengoal}}
• Kobiljar {{pengoal}}
• West {{pengoal}}
• Palushi {{pengoal}}
• Smoak {{pengoal}}
• Walker {{pengoal}}
• Redmore {{pengoal}}
• Penzone {{penmiss}}
• Abreu {{pengoal}}
• Rulle {{pengoal}}

|penalties2 =

• {{pengoal}} Grande Jr
• {{pengoal}} Espitia
• {{pengoal}} Esono
• {{pengoal}} Insúa
• {{pengoal}} Fraggetti
• {{pengoal}} Rivas
• {{pengoal}} Shelton
• {{penmiss}} Benito
• {{pengoal}} Callejas
• {{penmiss}} Occenat

|stadium = Hodges Stadium

|location = Jacksonville, Florida

|attendance = 3,526

|referee = Richonne Clark

}}

{{Football box collapsible

|date = March 23

|time = 7:30 pm EDT

|team1 = Hartford City FC (NPSL) {{flagicon|Connecticut}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/664892

|team2 = {{flagicon|New York}} Lansdowne Yonkers FC (LQ)

|goals1 =

• Guerra {{goal|28|pen.}}

|goals2 =

• Devivo {{goal|45+1||73}}

|stadium = Trinity Health Stadium

|location = Hartford, Connecticut

|attendance = 500

|referee = Thomas Snyder

}}

All times local to game site.

{{Football box collapsible

|date = April 4

|time = 7:00 PM EDT

|team1 = Miami FC (USLC) {{flagicon|Florida}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/miavsjac-04-04-2023/

|team2 = {{flagicon|Florida}} Jacksonville Armada U-23(NPSL)

|goals1 =

• Telfer {{goal|34}}
• Repetto {{goal|82||85|pen.}}

|goals2 =

• Eloundou {{goal|33}}

|stadium = FIU Soccer Stadium

|location = Miami, Florida

|attendance = 654

|referee = Jonathan Bilinski

}}

{{Football box collapsible

|date = April 4

|time = 7:00 PM EDT

|team1 = Hartford Athletic (USLC) {{flagicon|Connecticut}}

|score = 3–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/hfdvslyo-04-04-2023/

|team2 = {{flagicon|New York}} Lansdowne Yonkers FC (LQ)

|goals1 =

• Amoh {{goal|27||73|pen.|82}}

|goals2 =

|stadium = Trinity Health Stadium

|location = Hartford, Connecticut

|attendance = 500

|referee = Ernie Constantine

}}

{{Football box collapsible

|date = April 4

|time = 7:00 PM EDT

|team1 = One Knoxville SC (USL1) {{flagicon|Tennessee}}

|score = 1–2

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/knxvsmem-04-04-2023/

|team2 = {{flagicon|Tennessee}} Memphis 901 FC (USLC)

|goals1 =

• Ilić {{goal|57|pen.}}

|goals2 =

• Borczak {{goal|38}}
• Kelly {{goal|120+4}}

|stadium = Regal Soccer Stadium

|location = Knoxville, Tennessee

|attendance = 1,812

|referee = Matthew Thompson

}}

{{Football box collapsible

|date = April 4

|time = 7:00 PM EDT

|team1 = Charleston Battery (USLC) {{flagicon|South Carolina}}

|score = 4–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chsvsscl-04-04-2023/

|team2 = {{flagicon|Georgia (U.S. state)}} Savannah Clovers FC (NISA)

|goals1 =

• Crawford {{goal|37}}
• Williams {{goal|51}}
• Trager {{goal|63}}
• Markanich {{goal|77}}

|goals2 =

• Sanchez {{goal|76}}

|stadium = Patriots Point

|location = Mount Pleasant, South Carolina

|attendance = 1,617

|referee = Amiel Aleman

}}

{{Football box collapsible

|date = April 4

|time = 7:30 PM EDT

|team1 = Detroit City FC (USLC) {{flagicon|Michigan}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/detvsgsd-04-04-2023/

|team2 = {{flagicon|Michigan}} Gold Star FC Detroit (NISA)

|goals1 =

• Matthews {{goal|43}}

|goals2 =

|stadium = Keyworth Stadium

|location = Hamtramck, Michigan

|attendance = 1,361

|referee = Joshua Encarnación

}}

{{Football box collapsible

|date = April 4

|time = 7:30 PM EDT

|team1 = Tampa Bay Rowdies (USLC) {{flagicon|Florida}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/tbrvsnfc-04-04-2023/

|team2 = {{flagicon|Florida}} Nona FC (USL2)

|goals1 = Williams {{goal|26}}
Mkosana {{goal|33}}

|goals2 =

|stadium = Al Lang Stadium

|location = St. Petersburg, Florida

|attendance = 2,023

|referee = Joe Surgan

}}

{{Football box collapsible

|date = April 4

|time = 7:30 PM EDT

|team1 = Flower City Union (NISA) {{flagicon|New York (state)}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/fcuvsmsc-04-04-2023

|team2 = {{flagicon|New York (state)}} Manhattan SC (USL2)

|goals1 =

• Elias {{goal|36||42}}
• Diakhate {{goal|82}}

|goals2 =

• Karamoko {{goal|71}}

|stadium = Rochester Community Sports Complex Stadium

|location = Rochester, New York

|attendance = 751

|referee = Andrew Musashe

}}

{{Football box collapsible

|date = April 4

|time = 7:30 PM CDT

|team1 = San Antonio FC (USLC) {{flagicon|Texas}}

|score = 2–1

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/savscdl-04-04-2023/

|team2 = {{flagicon|Florida}} Club de Lyon (NISA)

|goals1 =

• Agustin {{goal|79}}
• Holt {{goal|98|pen.}}

|goals2 =

• Hernández {{goal|61}}

|stadium = Toyota Field

|location = San Antonio, Texas

|attendance = 3,204

|referee = Elton García

}}

{{Football box collapsible

|date = April 4

|time = 7:00 PM MDT

|team1 = New Mexico United (USLC) {{flagicon|New Mexico}}

|score = 6–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nmvsuda-04-04-2023/

|team2 = {{flagicon|New Mexico}} UDA Soccer (LQ)

|goals1 =

• Sosa {{goal|2}}
• Portillo {{goal|40|pen.}}
• Dolling {{goal|48||61|pen.|73}}
• Bruce {{goal|54}}

|goals2 =

|stadium = Rio Rancho High School

|location = Rio Rancho, New Mexico

|attendance = 1,680

|referee = Matt Geringer

}}

{{Football box collapsible

|date = April 4

|time = 7:00 PM PDT

|team1 = Oakland Roots SC (USLC) {{flagicon|California}}

|score = 3–1

|report = https://www.espn.com/soccer/match/_/gameId/667682

|team2 = {{flagicon|California}} El Farolito SC (NPSL)

|goals1 =

• Rodriguez {{goal|12}}
• Prentice {{goal|19||24}}

|goals2 =

• Delgado {{goal|45|pen.}}

|stadium = Pioneer Stadium

|location = Hayward, California

|attendance = 875

|referee = Christopher Calderon

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Charlotte Independence (USL1) {{flagicon|North Carolina}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cltvsafc-04-05-2023/

|team2 = {{flagicon|North Carolina}} Appalachian FC (NPSL)

|goals1 =

• Bennett {{goal|25|pen.}}
• Dimick {{goal|66}}

|goals2 =

|stadium = Mecklenburg County Sportsplex

|location = Matthews, North Carolina

|attendance = 2,564

|referee = Daniel Gutierrez

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Indy Eleven (USLC) {{flagicon|Indiana}}

|score =3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/indvsmcs-04-05-2023/

|team2 = {{flagicon|Michigan}} Michigan Stars FC (NISA)

|goals1 =

• Robledo {{goal|59}}
• Diz {{goal|92}}
• Tejada {{goal|106}}

|goals2 =

• Popovic {{goal|47}}

|stadium = Michael A. Carroll Stadium

|aet=yes|location = Indianapolis, Indiana

|attendance = 1,807

|referee = Alex Beehler

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Pittsburgh Riverhounds SC (USLC) {{flagicon|Pennsylvania}}

|score = 1–0
forfeit

|report = https://www.ussoccer.com/stories/2023/03/pittsburgh-riverhounds-through-to-open-cup-third-round-as-rochester-nyfc-ceases-operations

|team2 = {{flagicon|New York (state)}} {{Strikethrough|Rochester New York FC}} (MLSNP)

|goals1 =

|goals2 =

|stadium = Highmark Stadium

|location = Pittsburgh, Pennsylvania

|attendance =

|referee =

|note = Pittsburgh advanced on walkover due to Rochester New York FC ceasing operations on March 10, 2023.

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Louisville City FC (USLC) {{flagicon|Kentucky}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/louvslex-04-05-2023/

|team2 = {{flagicon|Kentucky}} Lexington SC (USL1)

|goals1 =

• Totsch {{goal|69}}

|goals2 =

|stadium = Lynn Family Stadium

|location = Louisville, Kentucky

|attendance = 4,205

|referee = Nabil Bensalah

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Loudoun United FC (USLC) {{flagicon|Virginia}}

|score = 2–1

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/ldnvsnc-04-05-2023/

|team2 = {{flagicon|North Carolina}} North Carolina FC (USL1)

|goals1 =

• Ryan {{goal|7}}
• Leggett {{goal|116}}

|goals2 =

• Servania {{goal|67}}

|stadium = Segra Field

|location = Leesburg, Virginia

|attendance = 2,779

|referee = Kenneth Rojas

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM EDT

|team1 = Maryland Bobcats FC (NISA) {{flagicon|Maryland}}

|score = 3–2

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/mbovsocn-04-05-2023/

|team2 = {{flagicon|New Jersey}} Ocean City Nor'easters (USL2)

|goals1 =

• Espinal {{goal|67|pen.|82}}
• Brown {{goal|120+3}}

|goals2 =

• Jackson {{goal|22}}
• Labutis {{goal|90+2}}

|stadium = Maryland SoccerPlex

|location = Boyds, Maryland

|attendance = 167

|referee = Luis Arroyo

}}

{{Football box collapsible

|date = April 5

|time = 7:30 PM EDT

|team1 = Chattanooga Red Wolves SC (USL1) {{flagicon|Tennessee}}

|score = 1–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chavsbhm-04-05-2023/

|team2 = {{flagicon|Alabama}} Birmingham Legion FC (USLC)

|goals1 =

• Lombardi {{goal|79}}

|goals2 =

• Agudelo {{goal|45+1||63}}
• Smith {{goal|47}}
• Nwegbo {{goal|87}}

|stadium = CHI Memorial Stadium

|location = East Ridge, Tennessee

|attendance = 500

|referee = Benjamin Meyer

}}

{{Football box collapsible

|date = April 5

|time = 7:30 PM EDT

|team1 = South Georgia Tormenta FC (USL1) {{flagicon|Georgia (U.S. state)}}

|score = 2–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/trmvsrgv-04-05-2023/

|team2 = {{flagicon|Texas}} Rio Grande Valley FC Toros (USLC)

|goals1 =

• Murphy {{goal|39}}
• Adjei {{goal|82}}

|goals2 =

• Ruiz {{goal|90+1}}

|stadium = Optim Sports Medicine Field

|location = Statesboro, Georgia

|attendance = 1,139

|referee = William Hale

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM CDT

|team1 = Des Moines Menace (USL2) {{flagicon|Iowa}}

|score = 1–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/dmmvscfc-04-05-2023/

|team2 = {{flagicon|Tennessee}} Chattanooga FC (NISA)

|goals1 =

• Garcia {{goal|69}}

|goals2 =

• Naglestad {{goal|23}}
• Rodriguez {{goal|35}}
• McGrath {{goal|72}}
• Louis {{goal|85}}

|stadium = Valley Stadium

|location = West Des Moines, Iowa

|attendance = 1,672

|referee = Chris Ruska

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM CDT

|team1 = Union Omaha (USL1) {{flagicon|Nebraska}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/omavselp-04-05-2023

|team2 = {{flagicon|Texas}} El Paso Locomotive FC (USLC)

|goals1 =

• Palacios {{goal|58}}
• dos Santos {{goal|75}}

|goals2 =

|stadium = Morrison Stadium

|location = Omaha, Nebraska

|attendance = 548

|referee = Velimir Stefanovic

}}

{{Football box collapsible

|date = April 5

|time = 7:00 CDT

|team1 = Tulsa Athletic (NPSL) {{flagicon|Oklahoma}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/tatvstul-04-05-2023/

|team2 = {{flagicon|Oklahoma}} FC Tulsa (USLC)

|goals1 =

• Harris {{goal|58}}

|goals2 =

|stadium = Athletic Community Field at Hicks Park

|location = Tulsa, Oklahoma

|attendance = 1,025

|referee = Brandon Gardner

}}

{{Football box collapsible

|date = April 5

|time = 7:00 MDT

|team1 = Colorado Springs Switchbacks FC (USLC) {{flagicon|Colorado}}

|score = 1–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cosvsnco-04-05-2023/

|team2 = {{flagicon|Colorado}} Northern Colorado Hailstorm FC (USL1)

|goals1 =

• Skundrich {{goal|82}}

|goals2 =

• Amann {{goal|88}}
• Benamna {{goal|95}}
• Hernández {{goal|107}}

|stadium = Weidner Field

|aet=yes|location = Colorado Springs, Colorado

|attendance = 200

|referee = Adam Rice

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM PDT

|team1 = Orange County SC (USLC) {{flagicon|California}}

|score = 5–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/ocvscap-04-05-2023/

|team2 = {{flagicon|California}} Capo FC (LQ)

|goals1 =

• Nielsen {{goal|33}}
• Jamison {{goal|46}}
• Iloski {{goal|69||93|pen.}}
• Amang {{goal|87}}

|goals2 =

|stadium = Championship Soccer Stadium

|location = Irvine, California

|attendance = 1,588

|referee =

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PDT

|team1 = Sacramento Republic FC (USLC) {{flagicon|California}}

|score = 5–4

|aet=yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/sacvscfr-04-05-2023/

|team2 = {{flagicon|Washington}} Crossfire Redmond (NPSL)

|goals1 =

• Lewis {{goal|81}}, {{goal|105}}
• Cicerone {{goal|85||97}}
• Fenwick {{goal|113}}

|goals2 =

• Aman {{goal|7||111}}
• Stewart {{goal|13}}
• Hussen {{goal|93}}

|stadium = Heart Health Park

|location = Sacramento, California

|attendance = 4,024

|referee = Turan Ozdemir

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM PDT

|team1 = San Diego Loyal SC (USLC) {{flagicon|California}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/sdvsasc-04-05-2023/

|team2 = {{flagicon|California}} Albion San Diego (NISA)

|goals1 =

• Adams {{goal|25|pen}}
• Conway {{goal|52}}

|goals2 =

|stadium = SDSU Sports Deck

|location = San Diego, California

|attendance = 1,200

|referee =

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM PDT

|team1 = Central Valley Fuego FC (USL1) {{flagicon|California}}

|score = 1–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cvvsmb-04-05-2023

|team2 = {{flagicon|California}} Monterey Bay FC (USLC)

|goals1 =

• Vasquez {{goal|116}}

|goals2 =

• Volesky {{goal|97}}
• Dixon {{goal|110}}
• Gleadle {{goal|120}}

|stadium = Fresno State Soccer Stadium

|aet=yes|location = Fresno, California

|attendance = 3,057

|referee =

}}

{{Football box collapsible

|date = April 5

|time = 7:00 PM PDT

|team1 = Las Vegas Lights FC (USLC) {{flagicon|Nevada}}

|score = 4–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/lvvslaf-04-05-2023/

|team2 = {{flagicon|California}} Los Angeles Force (NISA)

|goals1 =

• Gonzalez {{goal|16||23}}
• Ingram {{goal|37}}
• Torres {{goal|63}}

|goals2 =

|stadium = Peter Johann Soccer Field

|location = Las Vegas, Nevada

|attendance = 287

|referee = Brad Jensen

}}

{{Football box collapsible

|date = April 5

|time = 7:30 PM MST

|team1 = Phoenix Rising FC (USLC) {{flagicon|Arizona}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/phxvsgvl-04-05-2023/

|team2 = {{flagicon|South Carolina}} Greenville Triumph SC (USL1)

|goals1 =

• Harvey {{goal|21}}

|goals2 =

|stadium = Phoenix Rising Soccer Stadium

|location = Phoenix, Arizona

|attendance = 5,000

|referee = Brandon Stevis

}}

{{Football box collapsible

|date = April 6

|time = 7:00 EDT

|team1 = Richmond Kickers (USL1) {{flagicon|Virginia}}

|score =3–2

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/rmkvscls-04-06-2023/

|team2 = {{flagicon|Ohio}} Cleveland SC (NPSL)

|goals1 =

• Terzaghi {{goal|31||59}}
• Barnathan {{goal|79}}

|goals2 =

• Beck {{goal|80}}
• Harter {{goal|86}}

|stadium = City Stadium

|location = Richmond, Virginia

|attendance = 1,013

|referee = Christian Centeno

}}

{{Football box collapsible

|date = April 11

|time = 6:00 PM CDT

|team1 = Forward Madison FC (USL1) {{flagicon|Wisconsin}}

|score = 2–3

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/madvschi-04-11-2023/

|team2 = {{flagicon|Illinois}} Chicago House AC (LQ)

|goals1 =

• Onen {{goal|19}}
• Vásquez {{goal|48}}

|goals2 =

• Mann {{goal|75}}
• Smith Jr {{goal|90+2||103}}

|stadium = Breese Stevens Field

|location = Madison, Wisconsin

|attendance = 1,802

|referee = Calin Radosav

|note = Game rescheduled from its original date of April 4 due to expected inclement weather.{{cite web |url=https://www.forwardmadisonfc.com/news/2023/04/02/u-s-open-cup-match-between-forward-madison-fc-vs-chicago-house-ac-moved-to-april-11th/ |title=U.S. Open Cup Match Between Forward Madison FC vs Chicago House AC Moved to April 11th |last=Warwick |first=Evan |date=April 2, 2023 |website=ForwardMadisonFC.com |publisher=Forward Madison FC |access-date=April 2, 2023 |url-status=live |archive-url=https://web.archive.org/web/20230402202332/https://www.forwardmadisonfc.com/news/2023/04/02/u-s-open-cup-match-between-forward-madison-fc-vs-chicago-house-ac-moved-to-april-11th/ |archive-date=April 2, 2023}}

}}

The 30 second round winners and the 18 new teams from the MLS were pooled into 11 geographic regions for the third round draw that was conducted on April 6, 2023. All new MLS entrants were drawn to face a second round winner and no MLS vs. MLS match-ups were allowed in the third round. The 24 resulting matches were played on April 25 and 26.{{cite web |url=https://www.ussoccer.com/stories/2023/04/pots-set-for-todays-2023-lamar-hunt-us-open-cup-third-round-draw |title=POTS SET FOR 2023 LAMAR HUNT U.S. OPEN CUP THIRD ROUND DRAW |date=April 6, 2023 |website=USSoccer.com |publisher=U.S. Open Cup |access-date=April 7, 2023}}

All times local to game site.

{{Football box collapsible

|date = April 25

|time = 7:00 PM EDT

|team1 = Pittsburgh Riverhounds SC (USLC) {{flagicon|Pennsylvania}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/pitvsmbo-04-25-2023/

|team2 = {{flagicon|Maryland}} Maryland Bobcats FC (NISA)

|goals1 =

• Kizza {{goal|81}}
• Fahling {{goal|90+1}}

|goals2 =

|stadium = Highmark Stadium

|location = Pittsburgh, Pennsylvania

|attendance = 100

|referee = Alex Parent

}}

{{Football box collapsible

|date = April 25

|time = 7:00 PM EDT

|team1 = Charleston Battery (USLC) {{flagicon|South Carolina}}

|score = 1–0

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chsvsclt-04-25-2023/

|team2 = {{flagicon|North Carolina}} Charlotte Independence (USL1)

|goals1 =

• Markanich {{goal|112}}

|goals2 =

|stadium = Patriots Point Soccer Complex

|location = Mount Pleasant, South Carolina

|attendance = 1,151

|referee = Andrew Musashe

}}

{{Football box collapsible

|date = April 25

|time = 7:30 PM EDT

|team1 = New England Revolution (MLS) {{flagicon|Massachusetts}}

|score = 2–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nevshfd-04-25-2023/

|team2 = {{flagicon|Connecticut}} Hartford Athletic (USLC)

|goals1 =

• Rennicks {{goal|13}}
• Romney {{goal|76}}

|goals2 =

• Hoppenot {{goal|52}}

|stadium = Gillette Stadium

|location = Foxborough, Massachusetts

|attendance = 1,988

|referee = Jeremy Scheer

}}

{{Football box collapsible

|date = April 25

|time = 7:30 PM EDT

|team1 = Charlotte FC (MLS) {{flagicon|North Carolina}}

|score = 4–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cltvstrm-04-25-2023/

|team2 = {{flagicon|Georgia (U.S. state)}} South Georgia Tormenta FC (USL1)

|goals1 =

• Jóźwiak {{goal|24}}
• Copetti {{goal|55}}
• Jones {{goal|65}}
• Świderski {{goal|84}}

|goals2 =

• Cassini {{goal|83}}

|stadium = Sportsplex at Matthews

|location = Matthews, North Carolina

|attendance = 3,568

|referee = Jervis Atagana

}}

{{Football box collapsible

|date = April 25

|time = 7:30 PM EDT

|team1 = Detroit City FC (USLC) {{flagicon|Michigan}}

|score = 1–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/detvsmin-04-25-2023/

|team2 = {{flagicon|Minnesota}} Minnesota United FC (MLS)

|goals1 =

• Matthews {{goal|5}}

|goals2 =

• Fragapane {{goal|60}}
• Iwe {{goal|63}}
• Amarilla {{goal|66}}

|stadium = Keyworth Stadium

|location = Hamtramck, Michigan

|attendance = 4,529

|referee = Christian Little

}}

{{Football box collapsible

|date = April 25

|time = 7:00 PM CDT

|team1 = St. Louis City SC (MLS) {{flagicon|Missouri}}

|score = 5–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/stlvsoma-04-25-2023/

|team2 = {{flagicon|Nebraska}} Union Omaha (USL1)

|goals1 =

• Jackson {{goal|3||66}}
• Gallardo {{goal|48|o.g.}}
• Watts {{goal|62}}
• Löwen {{goal|86}}

|goals2 =

• Dolabella {{goal|79}}

|stadium = CityPark

|location = St. Louis, Missouri

|attendance = 22,423

|referee = Lorenzo Hernandez

}}

{{Football box collapsible

|date = April 25

|time = 7:30 PM CDT

|team1 = Sporting Kansas City (MLS) {{flagicon|Kansas}}

|score = 3–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/skcvstat-04-25-2023/

|team2 = {{flagicon|Oklahoma}} Tulsa Athletic (NPSL)

|goals1 =

• Sallói {{goal|32}}
• Pulido {{goal|54}}
• Tzionis {{goal|68}}

|goals2 =

|stadium = Children's Mercy Park

|location = Kansas City, Kansas

|attendance = 9,912

|referee = José Lara

}}

{{Football box collapsible

|date = April 25

|time = 7:00 PM PDT

|team1 = Monterey Bay FC (USLC) {{flagicon|California}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/mbvssj-04-25-2023/

|team2 = {{flagicon|California}} San Jose Earthquakes (MLS)

|goals1 =

• Volesky {{goal|26}}

|goals2 =

|stadium = Cardinale Stadium

|location = Seaside, California

|attendance = 4,010

|referee = Christopher Calderon

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM EDT

|team1 = Loudoun United FC (USLC) {{flagicon|Virginia}}

|score = 5–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/ldnvsfcu-04-26-2023/

|team2 = {{flagicon|New York}} Flower City Union (NISA)

|goals1 =

• Williamson {{goal|6||35}}
• Ryan {{goal|39}}
• Zanne {{goal|49}}
• ElMedkhar {{goal|61}}

|goals2 =

|stadium = Segra Field

|location = Leesburg, Virginia

|attendance = 250

|referee = Melvin Rivas

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM EDT

|team1 = FC Cincinnati (MLS) {{flagicon|Ohio}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cinvslou-04-26-2023/

|team2 = {{flagicon|Kentucky}} Louisville City FC (USLC)

|goals1 =

• Ordóñez {{goal|85}}

|goals2 =

|stadium = TQL Stadium

|location = Cincinnati, Ohio

|attendance = 10,062

|referee = Calin Radosav

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM MDT

|team1 = Colorado Rapids (MLS) {{flagicon|Colorado}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/colvsnco-04-26-2023/

|team2 = {{flagicon|Colorado}} Northern Colorado Hailstorm FC (USL1)

|goals1 =

• Lewis {{goal|5||55}}
• Max {{goal|65}}

|goals2 =

• Dietrich {{goal|40}}

|stadium = Dick's Sporting Goods Park

|location = Commerce City, Colorado

|attendance = 200

|referee = Brandon Stevis

}}

{{Football box collapsible

|date = April 26

|time = 7:30 pm EDT

|team1 = D.C. United (MLS) {{flagicon|Washington, D.C.}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/dcvsrmk-04-26-2023/

|team2 = {{flagicon|Virginia}} Richmond Kickers (USL1)

|goals1 =

• Asad {{goal|52|pen.}}

|goals2 =

|stadium = Audi Field

|location = Washington, D.C.

|attendance = 8,686

|referee = John Griggs

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM EDT

|team1 = Tampa Bay Rowdies (USLC) {{flagicon|Florida}}

|score = 0–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/tbrvshou-04-26-2023/

|team2 = {{flagicon|Texas}} Houston Dynamo FC (MLS)

|goals1 =

|goals2 =

• Raines {{goal|44}}

|stadium = Al Lang Stadium

|location = St. Petersburg, Florida

|attendance = 1,956

|referee = Elvis Osmanovic

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM EDT

|team1 = Miami FC (USLC) {{flagicon|Florida}}

|score = 2–2

|aet = yes

|penaltyscore = 3–5

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/miavsmia-04-26-2023/

|team2 = {{flagicon|Florida}} Inter Miami CF (MLS)

|goals1 =

• Sorto {{goal|3}}
• Yedlin {{goal|116|o.g.}}

|goals2 =

• Borgelin {{goal|89}}
• Sailor {{goal|118}}

|penalties1 =

• Hernández {{pengoal}}
• Craig {{pengoal}}
• Salazar {{pengoal}}
• Telfer {{penmiss}}

|penalties2 =

• {{pengoal}} Martínez
• {{pengoal}} Ulloa
• {{pengoal}} Negri
• {{pengoal}} Mota
• {{pengoal}} Miller

|stadium = Riccardo Silva Stadium

|location = Miami, Florida

|attendance = 12,099

|referee = Elton García

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM EDT

|team1 = Atlanta United FC (MLS) {{flagicon|Georgia (U.S. state)}}

|score = 1–2

|aet =yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/atlvsmem-04-26-2023/

|team2 = {{flagicon|Tennessee}} Memphis 901 FC (USLC)

|goals1 =

• Wolff {{goal|3}}

|goals2 =

• Goodrum {{goal|90+6|pen.}}
• Pickering {{goal|100}}

|stadium = Fifth Third Bank Stadium

|location = Kennesaw, Georgia

|attendance = 3,034

|referee = Aleksandar Zhelyazkov

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM EDT

|team1 = Columbus Crew (MLS) {{flagicon|Ohio}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/clbvsind-04-26-2023/

|team2 = {{flagicon|Indiana}} Indy Eleven (USLC)

|goals1 =

• Farsi {{goal|83}}

|goals2 =

|stadium = Lower.com Field

|location = Columbus, Ohio

|attendance = 2,000

|referee = Nabil Bensalah

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM CDT

|team1 = Birmingham Legion FC (USLC) {{flagicon|Alabama}}

|score = 1–1

|penaltyscore = 4–3

|aet = yes

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/bhmvscfc-04-26-2023/

|team2 = {{flagicon|Tennessee}} Chattanooga FC (NISA)

|goals1 =

• Kasim {{goal|31}}

|goals2 =

• Naglestad {{goal|68|pen.}}

|penalties1 =

• Pasher {{pengoal}}
• Agudelo {{penmiss}}
• Alves {{pengoal}}
• Rufe {{pengoal}}
• Martínez {{pengoal}}

|penalties2 =

• {{pengoal}} Naglestad
• {{penmiss}} Dixon
• {{pengoal}} Prepeliță
• {{pengoal}}Mwape
• {{penmiss}} Alvarenga

|stadium = University of Alabama Soccer Stadium

|location = Tuscaloosa, Alabama

|attendance = 150

|referee = Abdou Ndiaye

}}

{{Football box collapsible

|id=Chicago Fire FC Vs Chicago House AC|date = April 26

|time = 7:00 PM CDT

|team1 = Chicago Fire FC (MLS) {{flagicon|Illinois}}

|score = 3–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chivschi-04-26-2023/

|team2 = {{flagicon|Illinois}} Chicago House AC (LQ)

|goals1 =

• Przybyłko {{goal|26}}
• Burks {{goal|37||70}}

|goals2 =

|stadium = SeatGeek Stadium

|location = Bridgeview, Illinois

|attendance =3,421

|referee = Luis Arroyo

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM CDT

|team1 = Nashville SC (MLS) {{flagicon|Tennessee}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nshvssa-04-26-2023/

|team2 = {{flagicon|Texas}} San Antonio FC (USLC)

|goals1 =

• Zubak {{goal|71}}

|goals2 =

|stadium = Geodis Park

|location = Nashville, Tennessee

|attendance = 5,467

|referee = Sergii Demianchuk

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM MDT

|team1 = New Mexico United (USLC) {{flagicon|New Mexico}}

|score = 2–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nmvsphx-04-26-2023/

|team2 = {{flagicon|Arizona}} Phoenix Rising FC (USLC)

|goals1 =

• Hurst {{goal|42}}
• Dolling {{goal|87}}

|goals2 =

• Harvey {{goal|77}}

|stadium = Isotopes Park

|location = Albuquerque, New Mexico

|attendance = 4,126

|referee = William Hale

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM PDT

|team1 = Las Vegas Lights FC (USLC) {{flagicon|Nevada}}

|score = 1–3

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/lvvsrsl-04-26-2023/

|team2 = {{flagicon|Utah}} Real Salt Lake (MLS)

|goals1 =

• Stauffer {{goal|112}}

|goals2 =

• Kreilach {{goal|106||116}}
• Musovski {{goal|121}}

|stadium = Cashman Field

|location = Las Vegas, Nevada

|attendance = 300

|referee = Brandon Gardner

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM PDT

|team1 = Sacramento Republic FC (USLC) {{flagicon|California}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/sacvsoak-04-26-2023/

|team2 = {{flagicon|California}} Oakland Roots SC (USLC)

|goals1 =

• Ross {{goal|49}}

|goals2 =

|stadium = Heart Health Park

|location = Sacramento, California

|attendance = 4,379

|referee = Laura Rodriguez

}}

{{Football box collapsible

|date = April 26

|time = 7:00 PM PDT

|team1 = Seattle Sounders FC (MLS) {{flagicon|Washington}}

|score = 5–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/seavssd-04-26-2023/

|team2 = {{flagicon|California}} San Diego Loyal SC (USLC)

| goals1 =

• Montero {{goal|15||120+3|pen.}}
• Dobbelaere {{goal|26}}
• Rothrock {{goal|55}}
• Baker-Whiting {{goal|101}}

| goals2 =

• Corona {{goal|53|pen.|73}}
• Adams {{goal|90+3}}
• Simba {{goal|115}}

|stadium = Starfire Sports

|location = Tukwila, Washington

|attendance = 3,728

|referee = Cristian Campo Hernández

}}

{{Football box collapsible

|date = April 26

|time = 7:30 PM PDT

|team1 = Portland Timbers (MLS) {{flagicon|Oregon}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/porvsorc-04-26-2023/

|team2 = {{flagicon|California}} Orange County SC (USLC)

|goals1 =

• Loría {{goal|37}}
• Clegg {{goal|48}}
• Niezgoda {{goal|90+2}}

|goals2 =

• Iloski {{goal|54}}

|stadium = Providence Park

|location = Portland, Oregon

|attendance = 11,639

|referee = Olvin Oliva

}}

The winners of the Third Round and the new entrants to the tournament were grouped geographically into groups of four (eight groups). Each group had one of the MLS teams entering this round. Random selection for each group determined the pairs. Instances where geographical fit did not exist when creating groups was resolved by random selection.{{Cite web |date=February 2, 2022 |title=2022 US Open Cup Handbook Finalists Edition |url=https://cdn.ussoccer.com/-/media/project/ussf/open-cup/2022/2022opencuphandbookfinalistsedition-v120220202.ashx?la=en-us&rev=17e94fe0967445919973f80a3e3dcb00 |url-status=live |access-date=April 21, 2022 |website=US Soccer|archive-url=https://web.archive.org/web/20220420172559/https://cdn.ussoccer.com/-/media/project/ussf/open-cup/2022/2022opencuphandbookfinalistsedition-v120220202.ashx?la=en-us&rev=17e94fe0967445919973f80a3e3dcb00 |archive-date=April 20, 2022 }} The draw for the Round of 32 took place on April 27.

All times local to game site.

{{Football box collapsible

|date = May 9

|time = 7:00 PM EDT

|team1 = Inter Miami CF (MLS) {{flagicon|Florida}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/miavschs-05-09-2023/

|team2 = {{flagicon|South Carolina}} Charleston Battery (USLC)

|goals1 =

• Palma {{goal|48|o.g.}}

|goals2 =

|stadium = DRV PNK Stadium

|location = Fort Lauderdale, Florida

|attendance = 2,726

|referee = Jonathan Bilinski

}}

{{Football box collapsible

|date = May 9

|time = 7:00 PM EDT

|team1 = Charlotte FC (MLS) {{flagicon|North Carolina}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cltvsorl-05-09-2023/

|team2 = {{flagicon|Florida}} Orlando City SC (MLS)

|goals1 =

• Jóźwiak {{goal|70}}

|goals2 =

|stadium = Mecklenburg County Sportsplex at Matthews

|location = Matthews, North Carolina

|attendance = 4,024

|referee = Guido Gonzales Jr.

}}

{{Football box collapsible

|date = May 9

|time = 7:30 PM EDT

|team1 = New York Red Bulls (MLS) {{flagicon|New Jersey}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/rbnyvsdc-05-09-2023/

|team2 = {{flagicon|District of Columbia}} D.C. United (MLS)

|goals1 =

• Fernandez {{goal|28}}

|goals2 =

|stadium = MSU Soccer Park

|location = Montclair, New Jersey

|attendance = 2,396

|referee = Marcos de Oliveira

}}

{{Football box collapsible

|date = May 9

|time = 7:30 PM EDT

|team1 = New England Revolution (MLS) {{flagicon|Massachusetts}}

|score = 0–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nevspit-05-09-2023/

|team2 = {{flagicon|Pennsylvania}} Pittsburgh Riverhounds SC (USLC)

|goals1 =

|goals2 =

• Griffin {{goal|44}}

|stadium = Gillette Stadium

|location = Foxborough, Massachusetts

|attendance = 1,100

|referee = Matthew Thompson

}}

{{Football box collapsible

|date = May 9

|time = 7:00 PM CDT

|team1 = Chicago Fire FC (MLS) {{flagicon|Illinois}}

|score = 2–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chivsstl-05-09-2023/

|team2 = {{flagicon|Missouri}} St. Louis City SC (MLS)

|goals1 =

• Haile-Selassie {{goal|3}}
• Herbers {{goal|75}}

|goals2 =

• Perez {{goal|90+3}}

|stadium = SeatGeek Stadium

|location = Bridgeview, Illinois

|attendance = 4,371

|referee = Brandon Stevis

}}

{{Football box collapsible

|date = May 9

|time = 7:30 PM CDT

|team1 = Minnesota United FC (MLS) {{flagicon|Minnesota}}

|score = 3–3

|aet=Y

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/minvsphi-05-09-2023/

|team2 = {{flagicon|Pennsylvania}} Philadelphia Union (MLS)

|goals1 =

• Hlongwane {{goal|68||103}}
• Rosales {{goal|73}}

|goals2 =

• Donavan {{goal|78}}
• Wagner {{goal|90+4||120}}

|penaltyscore= 7–6

|penalties1=

• Fragapane {{pengoal}}
• Trapp {{pengoal}}
• Arriaga {{penmiss}}
• Rosales {{pengoal}}
• Dunbar {{pengoal}}
• Kallman {{pengoal}}
• Boxall {{pengoal}}
• Tapias {{pengoal}}

|penalties2=

• {{pengoal}} Gazdag
• {{pengoal}} Elliott
• {{penmiss}} Real
• {{pengoal}} Torres
• {{pengoal}} Carranza
• {{pengoal}} Rafanello
• {{pengoal}} Lowe
• {{penmiss}} Wagner

|stadium = Allianz Field

|location = Saint Paul, Minnesota

|attendance = 4,216

|referee = Fotis Bazakos

}}

{{Football box collapsible

|date = May 9

|time = 7:30 PM PDT

|team1 = Monterey Bay FC (USLC) {{flagicon|California}}

|score = 2–2

|aet=Y

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/mbvslafc-05-09-2023/

|team2 = {{flagicon|California}} Los Angeles FC (MLS)

|goals1 =

• Dawkins {{goal|90}}
• Maldonado {{goal|94}}

|goals2 =

• Torres {{goal|25}}
• Maia {{goal|105}}

|penaltyscore= 4–5

|penalties1=

• Volesky {{pengoal}}
• Dawkins {{pengoal}}
• Greene {{pengoal}}
• Okoli {{penmiss}}
• Boone {{pengoal}}
• Fehr {{penmiss}}

|penalties2=

• {{pengoal}} Maia
• {{pengoal}} Ordaz
• {{penmiss}} Crisostomo
• {{pengoal}} Moyado
• {{pengoal}} Jakupović
• {{pengoal}} Rosales

|stadium = Cardinale Stadium

|location = Seaside, California

|attendance = 5,808

|referee = Cristian Campo

}}

{{Football box collapsible

|date = May 9

|time = 7:30 PM PDT

|team1 = Sacramento Republic FC (USLC) {{flagicon|California}}

|score = 2–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/sacvscol-05-09-2023/

|team2 = {{flagicon|Colorado}} Colorado Rapids (MLS)

|goals1 =

• Herrera {{goal|30||52}}

|goals2 =

• Lewis {{goal|4}}
• Edwards {{goal|15}}
• Nicholson {{goal|55}}
• Barrios {{goal|81}}

|stadium = Heart Health Park

|location = Sacramento, California

|attendance = 8,897

|referee = Ricardo Fierro

}}

{{Football box collapsible

|date = May 10

|time = 7:00 PM EDT

|team1 = FC Cincinnati (MLS) {{flagicon|Ohio}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cinvsnyc-05-10-2023/

|team2 = {{flagicon|New York}} New York City FC (MLS)

|goals1 =

• Vázquez {{goal|56}}

|goals2 =

|stadium = TQL Stadium

|location = Cincinnati, Ohio

|attendance = 7,196

|referee = Timothy Ford

}}

{{Football box collapsible

|date = May 10

|time = 7:00 PM EDT

|team1 = Loudoun United FC (USLC) {{flagicon|Virginia}}

|score = 1–5

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/ldnvsclb-05-10-2023/

|team2 = {{flagicon|Ohio}} Columbus Crew (MLS)

|goals1 =

• ElMedkhar {{goal|86|pen.}}

|goals2 =

• Yeboah {{goal|6}}
• Zawadzki {{goal|18}}
• Parente {{goal|24||27}}
• Ramírez {{goal|36}}

|stadium = Segra Field

|location = Leesburg, Virginia

|attendance = 3,201

|referee = Luis Arroyo

}}

{{Football box collapsible

|date = May 10

|time = 7:00 PM CDT

|team1 = Birmingham Legion FC (USLC) {{flagicon|Alabama}}

|score = 3–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/bhmvsmem-05-10-2023/

|team2 = {{flagicon|Tennessee}} Memphis 901 FC (USLC)

|goals1 =

• Agudelo {{goal|20}}
• Martínez {{goal|39}}
• Pasher {{goal|64}}

|goals2 =

|stadium = Protective Stadium

|location = Birmingham, Alabama

|attendance = 3,150

|referee = Lorenzo Hernandez

}}

{{Football box collapsible

|date = May 10

|time = 7:30 PM CDT

|team1 = Houston Dynamo FC (MLS) {{flagicon|Texas}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/houvsskc-05-10-2023/

|team2 = {{flagicon|Kansas}} Sporting Kansas City (MLS)

|goals1 =

• Úlfarsson {{goal|12}}

|goals2 =

|stadium = Shell Energy Stadium

|location = Houston, Texas

|attendance = 5,094

|referee = Sergii Demianchuk

}}

{{Football box collapsible

|date = May 10

|time = 7:30 PM CDT

|team1 = Nashville SC (MLS) {{flagicon|Tennessee}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/nshvsdal-05-10-2023/

|team2 = {{flagicon|Texas}} FC Dallas (MLS)

|goals1 =

• Picault {{goal|63}}
• Muyl {{goal|76}}

|goals2 =

|stadium = Geodis Park

|location = Nashville, Tennessee

|attendance = 5,196

|referee = Lukasz Szpala

}}

{{Football box collapsible

|date = May 10

|time = 8:00 PM CDT

|team1 = Austin FC (MLS) {{flagicon|Texas}}

|score = 2–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/atxvsnm-05-10-2023/

|team2 = {{flagicon|New Mexico}} New Mexico United (USLC)

|goals1 =

• Redes {{goal|24}}
• Urruti {{goal|36}}

|goals2 =

|stadium = Q2 Stadium

|location = Austin, Texas

|attendance = 20,019

|referee = Elton García

}}

{{Football box collapsible

|date = May 10

|time = 7:30 PM PDT

|team1 = LA Galaxy (MLS) {{flagicon|California}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/lavssea-05-10-2023/

|team2 = {{flagicon|Washington}} Seattle Sounders FC (MLS)

|goals1 =

• Aude {{goal|3}}
• Rodríguez {{goal|66||84}}

|goals2 =

• Rothrock {{goal|68}}

|stadium = Dignity Health Sports Park

|location = Carson, California

|attendance = 8,412

|referee = Matt Geringer

}}

{{Football box collapsible

|date = May 10

|time = 7:30 PM PDT

|team1 = Portland Timbers (MLS) {{flagicon|Oregon}}

|score = 3–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/porvsrsl-05-10-2023/

|team2 = {{flagicon|Utah}} Real Salt Lake (MLS)

|goals1 =

• Blanco {{goal|4}}
• Rasmussen {{goal|50}}
• Niezgoda {{goal|52}}

|goals2 =

• Musovski {{goal|30}}
• Gómez {{goal|41}}
• Chang {{goal|55||59}}

|stadium = Providence Park

|location = Portland, Oregon

|attendance = 8,071

|referee = Victor Rivas

}}

{{16TeamBracket

| maxround =4

| seed-width = 40

| team-width = 165

| score-width = 30

| boldwinner = y

| RD1 = Round of 16
May 23–24

| RD2 = Quarterfinals
June 6–7

| RD3 = Semifinals
August 23

| RD4 = Final
September 27

| RD1-group1 =Northeast

| RD1-seed1 =MLS

| RD1-team1 =New York Red Bulls

| RD1-score1 =1 (3)

| RD1-seed2 =MLS

| RD1-team2 =FC Cincinnati {{pso}}

| RD1-score2 =1 (5)

| RD1-seed3 =USLC

| RD1-team3 =Pittsburgh Riverhounds SC

| RD1-score3 =1

| RD1-seed4 =MLS

| RD1-team4 =Columbus Crew

| RD1-score4 =0

| RD1-group2 =Southeast

| RD1-seed5 =USLC

| RD1-team5 =Birmingham Legion FC

| RD1-score5 =1

| RD1-seed6 =MLS

| RD1-team6 =Charlotte FC

| RD1-score6 =0

| RD1-seed7 =MLS

| RD1-team7 =Inter Miami CF

| RD1-score7 =2

| RD1-seed8 =MLS

| RD1-team8 =Nashville SC

| RD1-score8 =1

| RD1-group3 =Central

| RD1-seed9 =MLS

| RD1-team9 =Austin FC

| RD1-score9 =0

| RD1-seed10 =MLS

| RD1-team10 =Chicago Fire FC

| RD1-score10 =2

| RD1-seed11 =MLS

| RD1-team11 =Houston Dynamo FC

| RD1-score11 =4

| RD1-seed12 =MLS

| RD1-team12 =Minnesota United FC

| RD1-score12 =0

| RD1-group4 =West

| RD1-seed13 =MLS

| RD1-team13 =Colorado Rapids

| RD1-score13 = 0

| RD1-seed14 =MLS

| RD1-team14 =Real Salt Lake

| RD1-score14 = 1

| RD1-seed15 =MLS

| RD1-team15 =Los Angeles FC

| RD1-score15 = 0

| RD1-seed16 =MLS

| RD1-team16 =LA Galaxy

| RD1-score16 = 2

| RD2-seed1 =MLS

| RD2-team1 =FC Cincinnati

| RD2-score1 =3

| RD2-seed2 =USLC

| RD2-team2 =Pittsburgh Riverhounds SC

| RD2-score2 =1

| RD2-seed3 =USLC

| RD2-team3 =Birmingham Legion FC

| RD2-score3 =0

| RD2-seed4 =MLS

| RD2-team4 =Inter Miami CF

| RD2-score4 =1

| RD2-seed5 =MLS

| RD2-team5 =Chicago Fire FC

| RD2-score5 =1

| RD2-seed6 =MLS

| RD2-team6 =Houston Dynamo FC

| RD2-score6 =4

| RD2-seed7 =MLS

| RD2-team7 = Real Salt Lake

| RD2-score7 =3

| RD2-seed8 =MLS

| RD2-team8 = LA Galaxy

| RD2-score8 =2

| RD3-seed1 =MLS

| RD3-team1 =FC Cincinnati

| RD3-score1 =3 (4)

| RD3-seed2 =MLS

| RD3-team2 =Inter Miami CF {{pso}}

| RD3-score2 =3 (5)

| RD3-seed3 =MLS

| RD3-team3 =Houston Dynamo FC

| RD3-score3 =3 {{aet}}

| RD3-seed4 =MLS

| RD3-team4 =Real Salt Lake

| RD3-score4 =1

| RD4-seed1 =MLS

| RD4-team1 =Inter Miami CF

| RD4-score1 =1

| RD4-seed2 =MLS

| RD4-team2 =Houston Dynamo FC

| RD4-score2 =2

}}

Host team listed first

Bold = winner

(a.e.t.) = after extra time, (p) = penalty shootout score

All times local to game site.

{{Football box collapsible

|date = May 23

|time = 7:30 PM EDT

|team1 = Inter Miami CF (MLS) {{flagicon|Florida}}

|score = 2–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/miavsnsh-05-23-2023/

|team2 = {{flagicon|Tennessee}} Nashville SC (MLS)

|goals1 =

• Negri {{goal|57}}
• Stefanelli {{goal|73}}

|goals2 =

• Muyl {{goal|66}}

|stadium = DRV PNK Stadium

|location = Fort Lauderdale, Florida

|attendance = 2,244

|referee = Lukasz Szpala

}}

{{Football box collapsible

|date = May 23

|time = 7:30 PM EDT

|team1 = New York Red Bulls (MLS) {{flagicon|New Jersey}}

|score = 1–1

|aet=y

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/rbnyvscin-05-23-2023/

|team2 = {{flagicon|Ohio}} FC Cincinnati (MLS)

|goals1 =

• Vanzeir {{goal|90+2}}

|goals2 =

• Kubo {{goal|42}}

|penaltyscore=3–5

|penalties1=

• Vanzeir {{penmiss}}
• Manoel {{pengoal}}
• Carmona {{pengoal}}
• Cásseres {{pengoal}}

|penalties2=

• {{pengoal}} Acosta
• {{pengoal}} Moreno
• {{pengoal}} Badji
• {{pengoal}} Mosquera
• {{pengoal}} Barreal

|stadium = Red Bull Arena

|location = Harrison, New Jersey

|attendance = 4,131

|referee = Fotis Bazakos

}}

{{Football box collapsible

|date = May 23

|time = 7:30 PM CDT

|team1 = Houston Dynamo FC (MLS) {{flagicon|Texas}}

|score = 4–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/houvsmin-05-23-2023/

|team2 = {{flagicon|Minnesota}} Minnesota United FC (MLS)

|goals1 =

• Baird {{goal|33|pen.|68||89}}
• Ibrahim {{goal|79}}

|goals2 =

• Arriaga {{sent off|2|30|38}}

|stadium = Shell Energy Stadium

|location = Houston, Texas

|attendance = 2,947

|referee = Ramy Touchan

}}

{{Football box collapsible

|date = May 23

|time = 7:30 PM PDT

|team1 = Los Angeles FC (MLS) {{flagicon|California}}

|score = 0–2

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/lafcvsla-05-23-2023/

|team2 = {{flagicon|California}} LA Galaxy (MLS)

|goals1 =

|goals2 =

• Boyd {{goal|49}}
• Puig {{goal|52}}

|stadium = BMO Stadium

|location = Los Angeles, California

|attendance = 16,362

|referee = Allen Chapman

}}

{{Football box collapsible

|date = May 24

|time = 7:00 PM EDT

|team1 = Pittsburgh Riverhounds SC (USLC) {{flagicon|Pennsylvania}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/pitvsclb-05-24-2023/

|team2 = {{flagicon|Ohio}} Columbus Crew (MLS)

|goals1 =

• Dikwa {{goal|22}}
• Kizza {{sent off|0|90+5}}

|goals2 =

|stadium = Highmark Stadium

|location = Pittsburgh, Pennsylvania

|attendance = 6,107

|referee = Jeremy Scheer

}}

{{Football box collapsible

|date = May 24

|time = 7:00 PM CDT

|team1 = Birmingham Legion FC (USLC) {{flagicon|Alabama}}

|score = 1–0

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/bhmvsclt-05-24-2023/

|team2 = {{flagicon|North Carolina}} Charlotte FC (MLS)

|goals1 =

• Kasim {{goal|60}}

|goals2 =

• Malanda {{sent off|2|28|51}}

|stadium = Protective Stadium

|location = Birmingham, Alabama

|attendance = 12,722

|referee = Guido Gonzales Jr.

}}

{{Football box collapsible

|date = May 24

|time = 8:00 PM CDT

|team1 = Austin FC (MLS) {{flagicon|Texas}}

|score = 0–2

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/atxvschi-05-24-2023/

|team2 = {{flagicon|Illinois}} Chicago Fire FC (MLS)

|goals1 =

|goals2 =

• Czichos {{goal|27}}
• Przybyłko {{goal|77}}

|stadium = Q2 Stadium

|location = Austin, Texas

|attendance = 19,402

|referee = Alex Chilowicz

}}

{{Football box collapsible

|date = May 24

|time = 8:00 PM MDT

|team1 = Colorado Rapids (MLS) {{flagicon|Colorado}}

|score = 0–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/colvsrsl-05-24-2023/

|team2 = {{flagicon|Utah}} Real Salt Lake (MLS)

|goals1 =

|goals2 =

• Savarino {{goal|30}}

|stadium = Dick's Sporting Goods Park

|location = Commerce City, Colorado

|attendance = 2,280

|referee = Andrew Musashe

}}

All times local to game site.

{{Football box collapsible

|date = June 6

|time = 7:00 PM EDT

|team1 = FC Cincinnati (MLS) {{flagicon|Ohio}}

|score = 3–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cinvspit-06-06-2023/

|team2 = {{flagicon|Pennsylvania}} Pittsburgh Riverhounds SC (USLC)

|goals1 =

• Vázquez {{goal|56}}
• Barreal {{goal|71}}
• Arias {{goal|90+3}}

|goals2 =

• Showunmi {{goal|90+4}}

|stadium = TQL Stadium

|location = Cincinnati, Ohio

|attendance = 16,613

|referee = Elvis Osmanovic

}}

{{Football box collapsible

|date = June 6

|time = 7:30 PM CDT

|team1 = Chicago Fire FC (MLS) {{flagicon|Illinois}}

|score = 1–4

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/chivshou-06-06-2023/

|team2 = {{flagicon|Texas}} Houston Dynamo FC (MLS)

|goals1 =

• Souquet {{goal|40}}

|goals2 =

• Bassi {{goal|12|pen.}}
• Aliyu {{goal|31||59}}
• Quiñónes {{goal|74}}

|stadium = SeatGeek Stadium

|location = Bridgeview, Illinois

|attendance = 7,512

|referee = Marcos De Oliveira

}}

{{Football box collapsible

|date = June 7

|time = 7:00 PM CDT

|team1 = Birmingham Legion FC (USLC) {{flagicon|Alabama}}

|score =0–1

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/bhmvsmia-06-07-2023/

|team2 = {{flagicon|Florida}} Inter Miami CF (MLS)

|goals1 =

|goals2 =

• Stefanelli {{goal|56}}

|stadium = Protective Stadium

|location = Birmingham, Alabama

|attendance = 18,418

|referee = Sergii Demianchuk

}}

{{Football box collapsible

|date = June 7

|time = 7:30 PM MDT

|team1 = Real Salt Lake (MLS) {{flagicon|Utah}}

|score =3–2

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/rslvsla-06-07-2023/

|team2 = {{flagicon|California}} LA Galaxy (MLS)

|goals1 =

• Kreilach {{goal|19||45+6}}
• Savarino {{goal|51}}

|goals2 =

• Brugman {{goal|82|pen.}}
• Douglas Costa {{goal|84}}

|stadium = America First Field

|location = Sandy, Utah

|attendance = 20,712

|referee = Ramy Touchan

}}

All times local to game site.

{{Football box collapsible

|date = August 23

|time = 7:00 PM EDT

|team1 =FC Cincinnati (MLS) {{flagicon|Ohio}}

|score = 3–3

|aet = y

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/cinvsmia-08-23-2023/

|team2 ={{flagicon|Florida}} Inter Miami CF (MLS)

|goals1 =

• Acosta {{goal|18}}
• Vázquez {{goal|53}}
• Kubo {{goal|114}}

|goals2 =

• Campana {{goal|68||90+7}}
• Martínez {{goal|93}}

|penaltyscore = 4–5

|penalties1=

• Kubo {{pengoal}}
• Arias {{pengoal}}
• Santos {{pengoal}}
• Miazga {{pengoal}}
• Hagglund {{penmiss}}

|penalties2=

• {{pengoal}} Messi
• {{pengoal}} Farías
• {{pengoal}} Ruiz
• {{pengoal}} Martínez
• {{pengoal}} Cremaschi

|stadium = TQL Stadium

|location = Cincinnati, Ohio

|attendance = 25,513

|referee = Joseph Dickerson

}}

{{Football box collapsible

|date = August 23

|time = 8:30 PM CDT

|team1 = Houston Dynamo FC (MLS) {{flagicon|Texas}}

|score = 3–1

|aet = y

|report = https://www.mlssoccer.com/competitions/u-s-open-cup/2023/matches/houvsrsl-08-23-2023/

|team2 ={{flagicon|Utah}} Real Salt Lake (MLS)

|goals1 =

• Herrera {{goal|45+5}}
• Carrasquilla {{goal|105}}
• Caicedo {{goal|120+5}}

|goals2 =

• Julio {{goal|64}}

|stadium = Shell Energy Stadium

|location = Houston, Texas

|attendance = 11,137

|referee = Lukasz Szpala

}}

{{main|2023 U.S. Open Cup final}}

{{:2023 U.S. Open Cup final}}

Warner Bros. Discovery Sports holds the rights to broadcast the Open Cup from 2023 to 2030, as part of a larger media rights agreement with U.S. Soccer.{{cite press release |date=March 1, 2022 |title=Turner Sports and United States Soccer Federation Reach Multimedia Rights Agreement |url=https://www.ussoccer.com/stories/2022/03/turner-sports-and-united-states-soccer-federation-reach-multimedia-rights-agreement |publisher=United States Soccer Federation |access-date=March 22, 2022}}{{Cite web|url=https://worldsoccertalk.com/2022/02/15/cindy-parlow-cone-talks-us-soccer-media-rights/|title=Cindy Parlow Cone talks US Soccer media rights|first=Kartik|last=Krishnaiyer|date=February 15, 2022}} Select matches from the first three rounds were broadcast for free on the Bleacher Report app or via the B/R Football YouTube channel.{{cite press release |date=March 8, 2023 |title=Opening Rounds Of 2023 Lamar Hunt U.S. Open Cup To Stream On Bleacher Report App & B/R Football YouTube Channel |url=https://www.ussoccer.com/stories/2023/03/featured-games-of-the-2023-us-open-cup-to-be-on-bleacher-report-app-and-br-football-youtube-channel |publisher=United States Soccer Federation |access-date=March 9, 2023}} Select matches are sublicensed to stream live on an involved team's YouTube channel, Eleven Sports or the CBS Sports Golazo Network.{{Cite web|url=https://www.ussoccer.com/us-open-cup/2023-schedule-and-format|title=2023 U.S. Open Cup Schedule and Format|publisher=United States Soccer Federation |access-date=April 7, 2023|date=April 7, 2023}} The Round of 32 would see a majority of games featured on the Bleacher Report app and B/R Football YouTube channel, with four games on CBS Sports Golazo Network and four on the U.S. Soccer YouTube channel.{{cite press release| date= May 3, 2023 |title= All 16 Games of 2023 U.S. Open Cup Round of 32 to be Broadcast on B/R Platforms, CBS Sports Golazo Network and U.S. Soccer YouTube |url= https://www.ussoccer.com/stories/2023/05/all-games-of-2023-us-open-cup-round-of-32-broadcast-br-cbs-sports-golazo-and-us-soccer-youtube |publisher= United States Soccer Federation |access-date= May 6, 2023}} The Round of 16 and Quarterfinals had games split between Bleacher Report and CBS Sports Golazo Network.{{cite press release| date= May 19, 2023 |title= All 2023 U.S. Open Cup Round of 16 to Stream on CBS Sports Golazo Network and B/R Platforms| url= https://www.ussoccer.com/stories/2023/05/watch-every-2023-us-open-cup-round-of-16-match-on-cbs-sports-golazo-network-and-br-platforms |publisher= United States Soccer Federation |access-date= May 22, 2023}}{{cite press release|date= May 25, 2023 |title= 2023 U.S. Open Cup Quarterfinals Schedule Set for June 6-7| url=https://www.ussoccer.com/stories/2023/05/2023-us-open-cup-quarterfinals-schedule-set-for-june-6-7 |publisher= United States Soccer Federation |access-date= May 27, 2023}} The semifinals are planned to be streamed on both CBS Sports Golazo Network and Paramount+ in the United States as well as 20 international broadcasters due to Lionel Messi's signing with semifinalist Inter Miami CF, with the final airing on CBS Sports Network as well as streaming on Paramount+.{{cite press release |date=August 4, 2023 |title=U.S. Soccer Announces Global Broadcast Distribution For 2023 Lamar Hunt U.S. Open Cup Semifinals And Final |url=https://www.ussoccer.com/stories/2023/08/us-soccer-announces-global-broadcast-distribution-for-final-rounds-of-2023-us-open-cup |publisher=U.S. Soccer Federation |accessdate=August 6, 2023}} Spanish-language rights in the United States for the semifinals and final would belong to Telemundo and Universo, with both being streamed on Peacock.{{cite press release |date=August 9, 2023 |title=Telemundo Becomes Exclusive Spanish Language Home of 2023 U.S. Open Cup Semifinals and Final |url=https://www.ussoccer.com/stories/2023/08/telemundo-becomes-spanish-language-home-of-2023-us-open-cup-semifinals-and-final |publisher=U.S. Soccer Federation |accessdate=August 15, 2023}} In Argentina, it will be broadcast by TyC Sports only for Lionel Messi's Inter Miami matches.

{{legend|silver|Team eliminated for this round.}}

{{reflist}}

• {{official website|https://www.ussoccer.com/us-open-cup/2023-schedule-and-format}}

{{U.S. Open Cup}}

{{2023 in American men's soccer}}

{{2022–23 in North and Central America, and Caribbean football (CONCACAF)}}

Category:U.S. Open Cup

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U.S. Open Cup