AKAP12

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

AKAP12 has been shown to interact with Beta-2 adrenergic receptor.{{cite journal | vauthors = Fan G, Shumay E, Wang H, Malbon CC | title = The scaffold protein gravin (cAMP-dependent protein kinase-anchoring protein 250) binds the beta 2-adrenergic receptor via the receptor cytoplasmic Arg-329 to Leu-413 domain and provides a mobile scaffold during desensitization | journal = J. Biol. Chem. | volume = 276 | issue = 26 | pages = 24005–14 | date = Jun 2001 | pmid = 11309381 | doi = 10.1074/jbc.M011199200 | doi-access = free }}{{cite journal | vauthors = Shih M, Lin F, Scott JD, Wang HY, Malbon CC | title = Dynamic complexes of beta2-adrenergic receptors with protein kinases and phosphatases and the role of gravin | journal = J. Biol. Chem. | volume = 274 | issue = 3 | pages = 1588–95 | date = Jan 1999 | pmid = 9880537 | doi = 10.1074/jbc.274.3.1588 | doi-access = free }}

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• {{UCSC gene info|AKAP12}}

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• {{cite journal | vauthors = Lester LB, Scott JD | title = Anchoring and scaffold proteins for kinases and phosphatases | journal = Recent Prog. Horm. Res. | volume = 52 | pages = 409–29; discussion 429–30 | year = 1997 | pmid = 9238861 }}
• {{cite journal | vauthors = Michel JJ, Scott JD | title = AKAP mediated signal transduction | journal = Annu. Rev. Pharmacol. Toxicol. | volume = 42 | pages = 235–57 | year = 2002 | pmid = 11807172 | doi = 10.1146/annurev.pharmtox.42.083101.135801 }}
• {{cite journal | vauthors = Yildirim M, Paydas S, Tanriverdi K, Seydaoglu G, Disel U, Yavuz S | title = Gravin gene expression in acute leukaemias: clinical importance and review of the literature | journal = Leuk. Lymphoma | volume = 48 | issue = 6 | pages = 1167–72 | year = 2007 | pmid = 17577780 | doi = 10.1080/10428190701377055 | s2cid = 35975874 }}
• {{cite journal | vauthors = Gordon T, Grove B, Loftus JC, O'Toole T, McMillan R, Lindstrom J, Ginsberg MH | title = Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera | journal = J. Clin. Invest. | volume = 90 | issue = 3 | pages = 992–9 | year = 1992 | pmid = 1522245 | pmc = 329955 | doi = 10.1172/JCI115976 }}
• {{cite journal | vauthors = Sato N, Kokame K, Shimokado K, Kato H, Miyata T | title = Changes of gene expression by lysophosphatidylcholine in vascular endothelial cells: 12 up-regulated distinct genes including 5 cell growth-related, 3 thrombosis-related, and 4 others | journal = J. Biochem. | volume = 123 | issue = 6 | pages = 1119–26 | year = 1998 | pmid = 9604001 | doi = 10.1093/oxfordjournals.jbchem.a022051 }}
• {{cite journal | vauthors = Shih M, Lin F, Scott JD, Wang HY, Malbon CC | title = Dynamic complexes of beta2-adrenergic receptors with protein kinases and phosphatases and the role of gravin | journal = J. Biol. Chem. | volume = 274 | issue = 3 | pages = 1588–95 | year = 1999 | pmid = 9880537 | doi = 10.1074/jbc.274.3.1588 | doi-access = free }}
• {{cite journal | vauthors = Lin F, ((Wang Hy)), Malbon CC | title = Gravin-mediated formation of signaling complexes in beta 2-adrenergic receptor desensitization and resensitization | journal = J. Biol. Chem. | volume = 275 | issue = 25 | pages = 19025–34 | year = 2000 | pmid = 10858453 | doi = 10.1074/jbc.275.25.19025 | doi-access = free }}
• {{cite journal | vauthors = Fan G, Shumay E, Wang H, Malbon CC | title = The scaffold protein gravin (cAMP-dependent protein kinase-anchoring protein 250) binds the beta 2-adrenergic receptor via the receptor cytoplasmic Arg-329 to Leu-413 domain and provides a mobile scaffold during desensitization | journal = J. Biol. Chem. | volume = 276 | issue = 26 | pages = 24005–14 | year = 2001 | pmid = 11309381 | doi = 10.1074/jbc.M011199200 | doi-access = free }}
• {{cite journal | vauthors = Grove BD, Bruchey AK | title = Intracellular distribution of gravin, a PKA and PKC binding protein, in vascular endothelial cells | journal = J. Vasc. Res. | volume = 38 | issue = 2 | pages = 163–75 | year = 2001 | pmid = 11316952 | doi = 10.1159/000051043 | s2cid = 6002979 }}
• {{cite journal | vauthors = Sasaki H, Kunimatsu M, Fujii Y, Yamakawa Y, Fukai I, Kiriyama M, Nonaka M, Sasaki M | title = Autoantibody to gravin is expressed more strongly in younger and nonthymomatous patients with myasthenia gravis | journal = Surg. Today | volume = 31 | issue = 11 | pages = 1036–7 | year = 2001 | pmid = 11766078 | doi = 10.1007/s005950170020 | s2cid = 22167880 }}
• {{cite journal | vauthors = Piontek J, Brandt R | title = Differential and regulated binding of cAMP-dependent protein kinase and protein kinase C isoenzymes to gravin in human model neurons: Evidence that gravin provides a dynamic platform for the localization for kinases during neuronal development | journal = J. Biol. Chem. | volume = 278 | issue = 40 | pages = 38970–9 | year = 2003 | pmid = 12857743 | doi = 10.1074/jbc.M306749200 | doi-access = free }}
• {{cite journal | vauthors = Tao J, Wang HY, Malbon CC | title = Protein kinase A regulates AKAP250 (gravin) scaffold binding to the beta2-adrenergic receptor | journal = EMBO J. | volume = 22 | issue = 24 | pages = 6419–29 | year = 2003 | pmid = 14657015 | pmc = 291823 | doi = 10.1093/emboj/cdg628 }}
• {{cite journal | vauthors = Choi MC, Jong HS, Kim TY, Song SH, Lee DS, Lee JW, Kim TY, Kim NK, Bang YJ | title = AKAP12/Gravin is inactivated by epigenetic mechanism in human gastric carcinoma and shows growth suppressor activity | journal = Oncogene | volume = 23 | issue = 42 | pages = 7095–103 | year = 2004 | pmid = 15258566 | doi = 10.1038/sj.onc.1207932 | doi-access = free }}
• {{cite journal | vauthors = Ballif BA, Villén J, Beausoleil SA, Schwartz D, Gygi SP | title = Phosphoproteomic analysis of the developing mouse brain | journal = Mol. Cell. Proteomics | volume = 3 | issue = 11 | pages = 1093–101 | year = 2004 | pmid = 15345747 | doi = 10.1074/mcp.M400085-MCP200 | doi-access = free }}
• {{cite journal | vauthors = Streb JW, Miano JM | title = AKAP12alpha, an atypical serum response factor-dependent target gene | journal = J. Biol. Chem. | volume = 280 | issue = 6 | pages = 4125–34 | year = 2005 | pmid = 15590635 | doi = 10.1074/jbc.M412466200 | doi-access = free }}
• {{cite journal | vauthors = Streb JW, Miano JM | title = Cross-species sequence analysis reveals multiple charged residue-rich domains that regulate nuclear/cytoplasmic partitioning and membrane localization of a kinase anchoring protein 12 (SSeCKS/Gravin) | journal = J. Biol. Chem. | volume = 280 | issue = 30 | pages = 28007–14 | year = 2005 | pmid = 15923193 | doi = 10.1074/jbc.M414017200 | doi-access = free }}

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Category:A-kinase-anchoring proteins

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