ASF

• Alabama Shakespeare Festival, a drama festival
• Asimov's Science Fiction, a U.S.-based English-language science fiction magazine containing SF stories

• African swine fever virus (ASFV), the causative agent of African swine fever (ASF)
• Altered Schaedler flora, a standardized consortium of gastrointestinal microbial species
• Serine/arginine-rich splicing factor 1, a protein also known as alternative splicing factor 1 (ASF)

• Advanced Synchronization Facility, a proposed extension to the x86 instruction set architecture
• Advanced Systems Format (formerly "Advanced Streaming Format", .asf), a Microsoft streaming format
• Alert Standard Format, a protocol for remote management and control of systems in OS-absent environments
• Anti Spam Filtering, software and hardware protection layers used to fight email spam
• Apache Software Foundation, an umbrella non-profit organization that owns the Apache brandname
• APL (programming language) data files (file extension .asf)
• Atmel Software Framework, a set of source files, libraries and reference projects that aid in application development for Atmel microcontrollers

• Acrylic silicone fluoropolymer, a car wax chemical
• Air superiority fighter
• Alaska Satellite Facility, a NASA data center
• Amperes per square foot, a unit of current density often used in electroplating

• Acid Survivors Foundation, in Bangladesh
• Airports Security Force, an aviation security division in Pakistan
• African Standby Force, an international peace force controlled by the African Union
• The American-Scandinavian Foundation, a non-profit organization
• American Steel Foundries
• Army Service Forces, a 1940s-era US military command, distinct from the Army Air Forces or Army Ground Forces.
• ASF Mexico (American School Foundation, A.C.)
• Asian Sailing Federation
• Australian Softball Federation
• Australian Speleological Federation, a caving body in Australia
• Autism Science Foundation, a US non-profit organization
• Avocats Sans Frontières, an international non-governmental organization
• Superior Auditor of the Federation ({{langx|es|Auditoría Superior de la Federación|link=no}})
• Swiss Football Association ({{langx|fr|Association Suisse de Football|link=no}} and {{langx|it|Associazione Svizzera di Football|link=no}})

• Animal source foods, food items that come from an animal source
• alt.seduction.fast, a Usenet group
• Ashfield railway station, Glasgow, Scotland, National Rail designation
• Narimanovo Airport, Astrakhan, Russia, IATA code

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