Activity-based proteomics

The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group (RG, sometimes called a "warhead") and a tag. Additionally, some probes may contain a binding group which enhances selectivity. The reactive group usually contains a specially designed electrophile that becomes covalently-linked to a nucleophilic residue in the active site of an active enzyme. An enzyme that is inhibited or post-translationally modified will not react with an activity-based probe. The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with the Huisgen 1,3-dipolar cycloaddition (also known as click chemistry).{{cite journal | vauthors = Speers AE, Adam GC, Cravatt BF | title = Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition | journal = Journal of the American Chemical Society | volume = 125 | issue = 16 | pages = 4686–7 | date = April 2003 | pmid = 12696868 | doi = 10.1021/ja034490h }}

A major advantage of ABPP is the ability to monitor the availability of the enzyme active site directly, rather than being limited to protein or mRNA abundance. With classes of enzymes such as the serine hydrolases{{cite journal | vauthors = Liu Y, Patricelli MP, Cravatt BF | title = Activity-based protein profiling: the serine hydrolases | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 96 | issue = 26 | pages = 14694–9 | date = December 1999 | pmid = 10611275 | pmc = 24710 | doi = 10.1073/pnas.96.26.14694 | bibcode = 1999PNAS...9614694L | doi-access = free }} and metalloproteases{{cite journal | vauthors = Saghatelian A, Jessani N, Joseph A, Humphrey M, Cravatt BF | title = Activity-based probes for the proteomic profiling of metalloproteases | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 101 | issue = 27 | pages = 10000–5 | date = July 2004 | pmid = 15220480 | pmc = 454150 | doi = 10.1073/pnas.0402784101 | bibcode = 2004PNAS..10110000S | doi-access = free }} that often interact with endogenous inhibitors or that exist as inactive zymogens, this technique offers a valuable advantage over traditional techniques that rely on abundance rather than activity. Furthermore, ABPP could be used to target specific proteins which were previously viewed as undruggable targets.{{cite journal | url=https://doi.org/10.1016/j.ejmech.2020.112151 | doi=10.1016/j.ejmech.2020.112151 | title=Activity-based protein profiling: Recent advances in medicinal chemistry | date=2020 | last1=Deng | first1=Hui | last2=Lei | first2=Qian | last3=Wu | first3=Yangping | last4=He | first4=Yang | last5=Li | first5=Weimin | journal=European Journal of Medicinal Chemistry | volume=191 | pmid=32109778 }}

Image:Gel-abpp eg.png ABPP using probes with different fluorophores in the same lane to simultaneously profile differences in enzyme activities]]

In recent years ABPP has been combined with tandem mass spectrometry enabling the identification of hundreds of active enzymes from a single sample. This technique, known as ABPP-MudPIT (multidimensional protein identification technology) is especially useful for profiling inhibitor selectivity as the potency of an inhibitor can be tested against hundreds of targets simultaneously.{{cn|date=July 2024}}

ABPP were first reported in the 1990s in the study of proteases.{{cite journal | vauthors = Kam CM, Abuelyaman AS, Li Z, Hudig D, Powers JC | title = Biotinylated isocoumarins, new inhibitors and reagents for detection, localization, and isolation of serine proteases | journal = Bioconjugate Chemistry | volume = 4 | issue = 6 | pages = 560–7 | date = 1993 | pmid = 8305526 | doi = 10.1021/bc00024a021 }}{{cite journal | vauthors = Abuelyaman AS, Hudig D, Woodard SL, Powers JC | title = Fluorescent derivatives of diphenyl [1-(N-peptidylamino)alkyl]phosphonate esters: synthesis and use in the inhibition and cellular localization of serine proteases | journal = Bioconjugate Chemistry | volume = 5 | issue = 5 | pages = 400–5 | date = 1994 | pmid = 7849068 | doi = 10.1021/bc00029a004 }}

• Mass spectrometry
• Proteomics
• Related inhibitors MAFP and DIFP
• Chemoproteomics

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Category:Proteomics

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