Alpha-actinin-1

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha-actinin-1 is an F-actin cross-linking protein – a bundling protein that is thought to anchor actin to a number of intracellular structures.{{cite web |title=ACTN2 - Alpha-actinin-2 - Homo sapiens (Human) - ACTN2 gene & protein |url=https://www.uniprot.org/uniprot/P35609 |website=www.uniprot.org |access-date=5 December 2021 |language=en}} Alpha-actinin-1 is a non-muscle cytoskeletal isoform found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.{{cite web | title = Entrez Gene: ACTN1 actinin, alpha 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=87}}

Alpha-actinin-1 has been shown to interact with:

• CDK5R1,{{cite journal | vauthors = Dhavan R, Greer PL, Morabito MA, Orlando LR, Tsai LH | title = The cyclin-dependent kinase 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent manner | journal = J. Neurosci. | volume = 22 | issue = 18 | pages = 7879–91 |date=September 2002 | pmid = 12223541 | doi = 10.1523/JNEUROSCI.22-18-07879.2002| pmc = 6758084 }}
• CDK5R2,
• Collagen, type XVII, alpha 1,{{cite journal | vauthors = Gonzalez AM, Otey C, Edlund M, Jones JC | title = Interactions of a hemidesmosome component and actinin family members | journal = J. Cell Sci. | volume = 114 | issue = Pt 23 | pages = 4197–206 |date=December 2001 | doi = 10.1242/jcs.114.23.4197 | pmid = 11739652 | url = https://cdr.lib.unc.edu/downloads/d217qz52s }}
• GIPC1,{{cite journal | vauthors = Bunn RC, Jensen MA, Reed BC | title = Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton | journal = Mol. Biol. Cell | volume = 10 | issue = 4 | pages = 819–32 |date=April 1999 | pmid = 10198040 | pmc = 25204 | doi = 10.1091/mbc.10.4.819}}
• PDLIM1,{{cite journal | vauthors = Vallenius T, Luukko K, Mäkelä TP | title = CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 | journal = J. Biol. Chem. | volume = 275 | issue = 15 | pages = 11100–5 |date=April 2000 | pmid = 10753915 | doi = 10.1074/jbc.275.15.11100 | doi-access = free }}{{cite journal | vauthors = Bauer K, Kratzer M, Otte M, de Quintana KL, Hagmann J, Arnold GJ, Eckerskorn C, Lottspeich F, Siess W | title = Human CLP36, a PDZ-domain and LIM-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells | journal = Blood | volume = 96 | issue = 13 | pages = 4236–45 |date=December 2000 | doi = 10.1182/blood.V96.13.4236 | pmid = 11110697 }}
• Protein kinase N1,{{cite journal | vauthors = Feng S, Reséndiz JC, Christodoulides N, Lu X, Arboleda D, Berndt MC, Kroll MH | title = Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX complex | journal = Biochemistry | volume = 41 | issue = 4 | pages = 1100–8 |date=January 2002 | pmid = 11802708 | doi = 10.1021/bi0156005 }}
• SSX2IP,{{cite journal | vauthors = Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y | title = ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions | journal = J. Biol. Chem. | volume = 278 | issue = 6 | pages = 4103–11 |date=February 2003 | pmid = 12446711 | doi = 10.1074/jbc.M209832200 | doi-access = free }} and
• Zyxin.{{cite journal | vauthors = Reinhard M, Zumbrunn J, Jaquemar D, Kuhn M, Walter U, Trueb B | title = An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment | journal = J. Biol. Chem. | volume = 274 | issue = 19 | pages = 13410–8 |date=May 1999 | pmid = 10224105 | doi = 10.1074/jbc.274.19.13410 | doi-access = free }}{{cite journal | vauthors = Li B, Trueb B | title = Analysis of the alpha-actinin/zyxin interaction | journal = J. Biol. Chem. | volume = 276 | issue = 36 | pages = 33328–35 |date=September 2001 | pmid = 11423549 | doi = 10.1074/jbc.M100789200 | doi-access = free}}
• PTPRT (PTPrho){{cite journal| vauthors=Besco JA, Hooft van Huijsduijnen R, Frostholm A, Rotter A| title=Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT). | journal=Brain Res | year= 2006 | volume= 1116 | issue= 1 | pages= 50–7 | pmid=16973135 | doi=10.1016/j.brainres.2006.07.122 | s2cid=23343123 | url=https://pubmed.ncbi.nlm.nih.gov/16973135 }}

• Actinin

{{reflist}}

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• {{cite journal | vauthors=Glück U, Ben-Ze'ev A |title=Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells. |journal=J. Cell Sci. |volume=107 |issue= 7|pages= 1773–82 |year= 1995 |doi=10.1242/jcs.107.7.1773 |pmid= 7983147 }}
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• {{cite journal | vauthors=Egerton M, Moritz RL, Druker B |title=Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes. |journal=Biochem. Biophys. Res. Commun. |volume=224 |issue= 3 |pages= 666–74 |year= 1996 |pmid= 8713105 |doi= 10.1006/bbrc.1996.1082 |display-authors=etal}}
• {{cite journal | vauthors=Mukai H, Toshimori M, Shibata H |title=Interaction of PKN with alpha-actinin. |journal=J. Biol. Chem. |volume=272 |issue= 8 |pages= 4740–6 |year= 1997 |pmid= 9030526 |doi=10.1074/jbc.272.8.4740 |display-authors=etal|doi-access=free }}
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• {{cite journal | vauthors=Hazan RB, Norton L |title=The epidermal growth factor receptor modulates the interaction of E-cadherin with the actin cytoskeleton. |journal=J. Biol. Chem. |volume=273 |issue= 15 |pages= 9078–84 |year= 1998 |pmid= 9535896 |doi=10.1074/jbc.273.15.9078 |doi-access=free }}
• {{cite journal | vauthors=Bunn RC, Jensen MA, Reed BC |title=Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton. |journal=Mol. Biol. Cell |volume=10 |issue= 4 |pages= 819–32 |year= 1999 |pmid= 10198040 | pmc=25204 |doi= 10.1091/mbc.10.4.819}}
• {{cite journal | vauthors=Reinhard M, Zumbrunn J, Jaquemar D |title=An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment. |journal=J. Biol. Chem. |volume=274 |issue= 19 |pages= 13410–8 |year= 1999 |pmid= 10224105 |doi=10.1074/jbc.274.19.13410 |display-authors=etal|doi-access=free }}

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• [https://web.archive.org/web/20110722012214/http://cmkb.cellmigration.org/report.cgi?report=orth_overview&orth_acc=co00001603 Actinin, alpha 1] Info with links in the [https://web.archive.org/web/20141211173306/http://www.cellmigration.org/index.shtml Cell Migration Gateway]
• {{UCSC gene info|ACTN1}}

{{PDB Gallery|geneid=87}}

{{Cytoskeletal Proteins}}

Category:EF-hand-containing proteins