Analytical light scattering

Analytical light scattering (ALS), also loosely referred to as SEC-MALS, is the implementation of static light scattering (SLS) and dynamic light scattering (DLS) techniques in an online or flow mode. A typical ALS instrument consists of an HPLC/FPLC chromatography system coupled in-line with appropriate light scattering and refractive index detectors. The advantage of ALS over conventional steady-state light scattering methods is that it allows separation of molecules/macromolecules on a chromatography column prior to analysis with light scattering detectors. Accordingly, ALS enables one to determine hydrodynamic properties of a single monodisperse species as opposed to bulk or average measurements on a sample afforded by conventional light scattering.{{Cite journal |last=Some |first=Daniel |last2=Razinkov |first2=Vladimir |date=2019 |title=High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization |url=https://pubmed.ncbi.nlm.nih.gov/31267461/ |journal=Methods in Molecular Biology |volume=2025 |pages=335–359 |doi=10.1007/978-1-4939-9624-7_16 |issn=1940-6029 |pmid=31267461}}