Autoradiograph

In biology, this technique may be used to determine the tissue (or cell) localization of a radioactive substance, either introduced into a metabolic pathway, bound to a receptor{{cite journal |vauthors=Kuhar M, Yamamura HI |title=Localization of cholinergic muscarinic receptors in rat brain by light microscopic radioautography |journal=Brain Res. |volume=110 |issue=2 |pages=229–43 |date=Jul 1976 |pmid=938940 |doi=10.1016/0006-8993(76)90399-1|s2cid=36648292 }}{{cite journal |vauthors=Young WS, Kuhar MJ |title=A new method for receptor autoradiography: [3H]opioid receptors in rat brain |journal=Brain Res. |volume=179 |issue=2 |pages=255–70 |date=Dec 1979 |pmid=228806 |doi=10.1016/0006-8993(79)90442-6 |s2cid=21647100 }} or enzyme, or hybridized to a nucleic acid.{{cite journal |vauthors=Jin L, Lloyd RV |title=In situ hybridization: methods and applications |journal=J Clin Lab Anal |volume=11 |issue=1 |pages=2–9 |year=1997 |pmid=9021518 |pmc=6760707 |doi=10.1002/(SICI)1098-2825(1997)11:1<2::AID-JCLA2>3.0.CO;2-F}} Applications for autoradiography are broad, ranging from biomedical to environmental sciences to industry.

The use of radiolabeled ligands to determine the tissue distributions of receptors is termed either in vivo or in vitro receptor autoradiography if the ligand is administered into the circulation (with subsequent tissue removal and sectioning) or applied to the tissue sections, respectively.{{Cite book|last=Davenport|first=Anthony P.|date=2005-03-25|title=Receptor Binding Techniques|volume=306|url=http://dx.doi.org/10.1385/1592599273|doi=10.1385/1592599273|isbn=1-59259-927-3|s2cid=3691391}} Once the receptor density is known, in vitro autoradiography can also be used to determine the anatomical distribution and affinity of a radiolabeled drug towards the receptor. For in vitro autoradiography, radioligand was directly applying on frozen tissue sections without administration to the subject. Thus it cannot follow the distribution, metabolism and degradation situation completely in the living body. But because target in the cryosections is widely exposed and can direct contact with radioligand, in vitro autoradiography is still a quick and easy method to screen drug candidates, PET and SPECT ligands. The ligands are generally labeled with ³H (tritium), ¹⁸F (fluorine), ¹¹C (carbon) or ¹²⁵I (radioiodine). Compare to in vitro, ex vivo autoradiography were performed after administration of radioligand in the body, which can decrease the artifacts and are closer to the inner environment.

The distribution of RNA transcripts in tissue sections by the use of radiolabeled, complementary oligonucleotides or ribonucleic acids ("riboprobes") is called in situ hybridization histochemistry. Radioactive precursors of DNA and RNA, [³H]-thymidine and [³H]-uridine respectively, may be introduced to living cells to determine the timing of several phases of the cell cycle. RNA or DNA viral sequences can also be located in this fashion. These probes are usually labeled with ³²P, ³³P, or ³⁵S. In the realm of behavioral endocrinology, autoradiography can be used to determine hormonal uptake and indicate receptor location; an animal can be injected with a radiolabeled hormone, or the study can be conducted in vitro.

The rate of DNA replication in a mouse cell growing in vitro was measured by autoradiography as 33 nucleotides per second.{{cite journal |vauthors=Hand R |title=Deoxyribonucleic acid fiber autoradiography as a technique for studying the replication of the mammalian chromosome |journal=J. Histochem. Cytochem. |volume=23 |issue=7 |pages=475–81 |year=1975 |pmid=1095649 |doi= 10.1177/23.7.1095649|doi-access=free }} The rate of phage T4 DNA elongation in phage-infected E. coli was also measured by autoradiography as 749 nucleotides per second during the period of exponential DNA increase at {{convert|37|C}}.{{cite journal | vauthors = McCarthy D, Minner C, Bernstein H, Bernstein C | year = 1976 | title = DNA elongation rates and growing point distributions of wild-type phage T4 and a DNA-delay amber mutant | journal = J Mol Biol | volume = 106 | issue = 4| pages = 963–81 | pmid = 789903 | doi=10.1016/0022-2836(76)90346-6}}

Phosphorylation means the posttranslational addition of a phosphate group to specific amino acids of proteins, and such modification can lead to a drastic change in the stability or the function of a protein in the cell. Protein phosphorylation can be detected on an autoradiograph, after incubating the protein in vitro with the appropriate kinase and γ-32P-ATP. The radiolabeled phosphate of latter is incorporated into the protein which is isolated via SDS-PAGE and visualized on an autoradiograph of the gel. (See figure 3. of a recent study showing that CREB-binding protein is phosphorylated by HIPK2.{{cite journal | vauthors = Kovacs KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux JR | title = Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2 | journal = Cell Signaling | volume = 27 | issue = 11 | pages = 2252–60 | date = Nov 2015 | pmid = 26247811 | doi = 10.1016/j.cellsig.2015.08.001 | url = https://repository.ist.ac.at/578/1/CLS-D-15-00072R1_.pdf }})

In plant physiology, autoradiography can be used to determine sugar accumulation in leaf tissue.{{Cite journal|last1=Goggin|first1=Fiona L.|last2=Medville|first2=Richard|last3=Turgeon|first3=Robert|date=2001-02-01|title=Phloem Loading in the Tulip Tree. Mechanisms and Evolutionary Implications|journal=Plant Physiology|volume=125|issue=2|pages=891–899|doi=10.1104/pp.125.2.891|pmid=11161046|pmc=64890|issn=0032-0889}} Sugar accumulation, as it relates to autoradiography, can described the phloem-loading strategy used in a plant.{{Cite journal|last=Van Bel|first=A J E|date=June 1993|title=Strategies of Phloem Loading|journal=Annual Review of Plant Physiology and Plant Molecular Biology|volume=44|issue=1|pages=253–281|doi=10.1146/annurev.pp.44.060193.001345|issn=1040-2519}} For example, if sugars accumulate in the minor veins of a leaf, it is expected that the leaves have few plasmodesmatal connections which is indicative of apoplastic movement, or an active phloem-loading strategy. Sugars, such as sucrose, fructose, or mannitol, are radiolabeled with [14-C], and then absorbed into leaf tissue by simple diffusion.{{Cite journal|last1=Turgeon|first1=R.|last2=Medville|first2=R.|date=1998-09-29|title=The absence of phloem loading in willow leaves|journal=Proceedings of the National Academy of Sciences|volume=95|issue=20|pages=12055–12060|doi=10.1073/pnas.95.20.12055|pmid=9751789|pmc=21764|bibcode=1998PNAS...9512055T |issn=0027-8424|doi-access=free}} The leaf tissue is then exposed to autoradiographic film (or emulsion) to produce an image. Images will show distinct vein patterns if sugar accumulation is concentrated in leaf veins (apoplastic movement), or images will show a static-like pattern if sugar accumulation is uniform throughout the leaf (symplastic movement).

This autoradiographic approach contrasts to techniques such as PET and SPECT where the exact 3-dimensional localization of the radiation source is provided by careful use of coincidence counting, gamma counters and other devices.

Krypton-85 is used to inspect aircraft components for small defects. Krypton-85 is allowed to penetrate small cracks, and then its

presence is detected by autoradiography. The method is called "krypton gas penetrant imaging". The gas penetrates smaller openings than the liquids used in dye penetrant inspection and fluorescent penetrant inspection.{{Cite web|url=http://www.asnt.org/publications/materialseval/solution/decsolutions/decsolutions.htm|archiveurl=https://web.archive.org/web/20080720084341/http://www.asnt.org/publications/materialseval/solution/decsolutions/decsolutions.htm|url-status=dead|title=Krypton Gas Penetrant Imaging - A Valuable Tool for Ensuring Structural Integrity in Aircraft Engine Components|archivedate=July 20, 2008}}

File:Crossroads Radioactive Puffy Surgeon Fish.jpg makes its own X-ray. The bright area is a meal of fresh algae. The rest of the body has absorbed and distributed enough plutonium to make the scales radioactive. The fish was alive and apparently healthy when captured.]]

The task of radioactive decontamination following the Baker nuclear test at Bikini Atoll during Operation Crossroads in 1946 was far more difficult than the U.S. Navy had prepared for. Though the task's futility became apparent and the danger to cleanup crews mounted, Colonel Stafford Warren, in charge of radiation safety, had difficulty persuading Vice Admiral William H. P. Blandy to abandon the cleanup and with it the surviving target ships. On August 10, Warren showed Blandy an autoradiograph made by a surgeonfish from the lagoon that was left on a photographic plate overnight. The film was exposed by alpha radiation produced from the fish's scales, evidence that plutonium, mimicking calcium, had been distributed throughout the fish. Blandy promptly ordered that all further decontamination work be discontinued. Warren wrote home, "A self X ray of a fish ... did the trick."{{citation| last = Weisgall | first = Jonathan| year = 1994| title = Operation Crossroads: The Atomic Tests at Bikini Atoll|location = Annapolis, Maryland|publisher = Naval Institute Press|isbn = 978-1-55750-919-2|page = [https://archive.org/details/operationcrossro0000weis/page/242 242]| url=https://archive.org/details/operationcrossro0000weis/page/242}}

Original publication by sole inventor

Askins, Barbara S. (1 November 1976). "Photographic image intensification by autoradiography". Applied Optics. 15 (11): 2860–2865. Bibcode:1976ApOpt..15.2860A. doi:10.1364/ao.15.002860.

{{Reflist}}

• {{cite book |title=Techniques of Autoradiography |last=Rogers |first=Andrew W |year=1979 |edition=3rd |publisher=Elsevier North Holland |location=New York |isbn=978-0-444-80063-3 }}
• "[https://patents.google.com/patent/US4101780 Patent US4101780 Treating silver with a radioactive sulfur compound such as thiourea or derivatives]". Google Patents. Retrieved 26 June 2014.

{{Nuclear technology}}

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Category:Radiobiology

Category:Radiography