BAF agar
{{Short description|Agar media containing peptones}}
BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi.{{Cite journal |last1=Águeda |first1=Beatriz |last2=Parladé |first2=Javier |last3=Fernández-Toirán |first3=Luz Marina |last4=Cisneros |first4=Óscar |last5=de Miguel |first5=Ana María |last6=Modrego |first6=María Pilar |last7=Martínez-Peña |first7=Fernando |last8=Pera |first8=Joan |date=October 2008 |title=Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.) |url=http://link.springer.com/10.1007/s00572-008-0192-3 |journal=Mycorrhiza |language=en |volume=18 |issue=8 |pages=443–449 |doi=10.1007/s00572-008-0192-3 |pmid=18695982 |bibcode=2008Mycor..18..443A |s2cid=11233267 |issn=0940-6360|url-access=subscription }} It was first described by {{ill|A.J.P. Oort|qid=Q21484444}} in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.
Typical composition
BAF agar typically contains:{{Cite web |title=CBS List of Media |url=https://usccn.org/wp-content/uploads/2020/08/CBS-media-list.pdf}}
- 30.0 g/L glucose
- 2.0 g/L peptone
- 0.2 g/L yeast extract
- 0.5 g/L KH2PO4
- 0.5 g/L MgSO4.7 H2O
- 10.0 mg/L FeCl3.6 H2O
- 1.0 mg/L ZnSO4.7 H2O
- 5.0 mg/L MnSO4
- 100.0 mg/L CaCl2.2 H2O
- 50.0 μg/L thiamine HCl
- 1.0 μg/L biotin
- 100.0 μg/L folic acid
- 50.0 μg/L inositol
- 15 g/L agar