Basophil#Clinical significance

Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope when stained. However, when unstained, the nucleus is visible and it usually has two lobes.{{cite web |title=Basophil |url=http://medcell.med.yale.edu/histology/blood_bone_marrow_lab/basophil.php |website=medcell.med.yale.edu |access-date=2019-03-15 |archive-date=2020-07-03 |archive-url=https://web.archive.org/web/20200703124059/http://medcell.med.yale.edu/histology/blood_bone_marrow_lab/basophil.php |url-status=dead }} The mast cell, another granulocyte, is similar in appearance and function. Both cell types store histamine, a chemical that is secreted by the cells when stimulated. However, they arise from different branches of hematopoiesis, and mast cells usually do not circulate in the blood stream, but instead are located in connective tissue. Like all circulating granulocytes, basophils can be recruited out of the blood into a tissue when needed.

File:Reference ranges for blood tests - white blood cells.png of white blood cells, comparing basophil amount (shown in violet) with other cells.]]

Basophils appear in many specific kinds of inflammatory reactions, particularly those that cause allergic symptoms. Basophils contain anticoagulant heparin,{{cite journal|last1=Lim|first1=Gregory|url=https://www.nature.com/articles/nrcardio.2017.171/|title=Discovery and purification of heparin|journal=Nature Reviews Cardiology|date=17 December 2017|doi=10.1038/nrcardio.2017.171 }} which prevents blood from clotting too quickly. They also contain the vasodilator histamine, which promotes blood flow to tissues. They can be found in unusually high numbers at sites of [https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ectoparasite ectoparasite] infection (e.g., ticks).

Like eosinophils, basophils play a role in both parasitic infections and allergies.{{cite journal | vauthors = Voehringer D | title = The role of basophils in helminth infection | journal = Trends in Parasitology | volume = 25 | issue = 12 | pages = 551–6 | date = December 2009 | pmid = 19782643 | doi = 10.1016/j.pt.2009.09.004 }} They are found in tissues where allergic reactions are occurring and probably contribute to the severity of these reactions. Basophils have protein receptors on their cell surface that bind IgE, an immunoglobulin involved in macroparasite defense and allergy. It is the bound IgE antibody that confers a selective response of these cells to environmental substances (e.g., pollen proteins or helminth antigens).

Recent studies in mice suggest that basophils may also regulate the behavior of T cells and mediate the magnitude of the secondary immune response.{{cite journal | vauthors = Nakanishi K | title = Basophils as APC in Th2 response in allergic inflammation and parasite infection | journal = Current Opinion in Immunology | volume = 22 | issue = 6 | pages = 814–20 | date = December 2010 | pmid = 21095110 | doi = 10.1016/j.coi.2010.10.018 }}

Basophil function is inhibited by CD200. Herpesvirus-6, herpesvirus-7, and herpesvirus-8 produce a CD200 homolog which also inhibits basophil function. This suggests that basophils may play a role in the immune response to these viruses.{{cite journal | vauthors = Shiratori I, Yamaguchi M, Suzukawa M, Yamamoto K, Lanier LL, Saito T, Arase H | title = Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200 | journal = Journal of Immunology | volume = 175 | issue = 7 | pages = 4441–9 | date = October 2005 | pmid = 16177086 | doi = 10.4049/jimmunol.175.7.4441 | doi-access = free }} The role of basophils in the immune response to these viruses is further supported by findings that the CD200 receptor is expressed more frequently in basophils than in other circulating leukocytes.{{cite journal |last1=Torrero |first1=Marina |last2=Larson |first2=David |last3=Hübner |first3=Marc |last4=Mitre |first4=Edson |title=CD200R surface expression as a marker of murine basophil activation |journal=Clinical & Experimental Allergy |date=March 2009 |volume=39 |issue=3 |pages=361–369 |doi=10.1111/j.1365-2222.2008.03154.x |pmid=19134017 |pmc=2743132 }}

Basophils arise and mature in bone marrow. When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic enzymes (e.g. elastase and lysophospholipase). They also secrete lipid mediators like leukotrienes (LTD-4), and several cytokines. Histamine and proteoglycans are pre-stored in the cell's granules while the other secreted substances are newly generated. Each of these substances contributes to inflammation. Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. Interleukin-4 is considered one of the critical cytokines in the development of allergies and the production of IgE antibody by the immune system. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation.{{cite book | author = Janeway CA Jr. | author-link = Charles Janeway | title = Immunobiology. | edition = 5th | publisher = Garland Publishing | year = 2001 | url = https://archive.org/details/immunobiology00char | isbn = 978-0-8153-3642-6 | format = electronic full text via NCBI Bookshelf | display-authors = etal | url-access = registration }}

The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test (BAT). Especially, in the diagnosis of allergies including of drug reactions (e.g. induced by contrast medium), the BAT is of great impact.{{cite journal | vauthors = Böhm I, Speck U, Schild HH | title = Pilot study on basophil activation induced by contrast medium | journal = Fundamental & Clinical Pharmacology | volume = 25 | issue = 2 | pages = 267–76 | date = April 2011 | pmid = 20412314 | doi = 10.1111/j.1472-8206.2010.00826.x | s2cid = 20951263 }}

Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in association with autoimmune urticaria{{cite journal | vauthors = Grattan CE, Dawn G, Gibbs S, Francis DM | title = Blood basophil numbers in chronic ordinary urticaria and healthy controls: diurnal variation, influence of loratadine and prednisolone and relationship to disease activity | journal = Clinical and Experimental Allergy | volume = 33 | issue = 3 | pages = 337–41 | date = March 2003 | pmid = 12614448 | doi = 10.1046/j.1365-2222.2003.01589.x | s2cid = 30477970 }} (a chronic itching condition). Basophilia is also uncommon but may be seen in some forms of leukemia or lymphoma.

Basophils of mice and humans have consistent immunophenotypes, including FcεRI⁺, CD123, CD49b(DX-5)⁺, CD69⁺, Thy-1.2⁺, 2B4⁺, CD11b^dull, CD117(c-kit)⁻, CD24⁻, CD19⁻, CD80⁻, CD14⁻, CD23⁻, Ly49c⁻, CD122⁻, CD11c⁻, Gr-1⁻, NK1.1⁻, B220⁻, CD3⁻, γδTCR⁻, αβTCR⁻, α₄ and β₄-integrin negative.{{cite book |author=Schroeder JT |title=Chapter 4 Basophils |year=2009 |volume=101 |pages=123–161 |pmid=19231594 |doi=10.1016/S0065-2776(08)01004-3|series=Advances in Immunology |isbn=9780123747938 }}

Recently, Heneberg{{cite journal | vauthors = Heneberg P | title = Mast cells and basophils: trojan horses of conventional lin- stem/progenitor cell isolates | journal = Current Pharmaceutical Design | volume = 17 | issue = 34 | pages = 3753–71 | date = November 2011 | pmid = 22103846 | doi = 10.2174/138161211798357881 }} proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69, CD107a, CD123, CD164, CD193/ CCR3, CD203c, TLR-4, and FcεRI. When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, and the ectoenzyme CD203c).

Basophils are easily isolated from venous blood and present good "indicator cells" of an IgE-mediated allergic response based on the upregulation of activation markers such as CD63 and/or CD203c upon suspect allergen stimulation.{{cite journal | vauthors = Hoffmann HJ et al. | title = The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease | journal = Allergy | volume = 70 | pages = 1393–1405 | date = 2015| issue = 11 | pmid = 26198455 | doi = 10.1111/all.12698 | hdl = 1874/333232 | s2cid = 24598924 | hdl-access = free }} Therefore, the BAT serves to confirm IgE-mediated allergy following uncertain results from classical testing based on anamnesis, skin testing or specific IgE results. More recently, BAT has also been used for the monitoring of successful allergen immunotherapy (desensitization) to differentiate short-term desensitization versus sustained unresponsiveness to the allergen.{{cite journal | vauthors = Paranjape A et al. | title = Oral Immunotherapy and Basophil and Mast Cell Reactivity in Food Allergy | journal = Frontiers in Immunology | volume = 11 | pages = 3228 | date = 2020| pmid = 33381123| doi = 10.3389/fimmu.2020.602660 | pmc = 7768812 | doi-access = free }}

The word basophil uses combining forms of baso- + -phil, yielding "base-loving".

File:Illu blood cell lineage.jpg|Blood cell lineage

File:Basophil.jpg|Alternate stain of a basophil

{{Portal|Biology|Medicine}}

• Allergy
• Diamine oxidase
• Eosinophil
• Food intolerance
• Histamine
• Histamine intolerance
• Histamine N-methyltransferase or HNMT
• Mast cell
• List of distinct cell types in the adult human body

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