C7orf61

C7orf61 is located on the reverse (or negative) DNA strand and is situated in chromosome 7 (7q22.1) from base pairs 100,456,615-100,464,271 - roughly 7,656 bp.{{cite web | url=https://www.ncbi.nlm.nih.gov/protein/NP_001004323.1 | title=NCBI h. sapiens Protein C7orf61 | access-date=4 January 2018}} It has a total of 3 exons and lacks isoforms.

The mRNA is approximately 1019 bp and belongs to domain of unknown function 4703 (PFAM15775).{{cite web | url=https://www.ncbi.nlm.nih.gov/nuccore/NM_001004323.2 | title=NCBI h. sapiens C7orf61 mRNA| access-date=20 February 2018}}

In humans, the protein contains a total of 206 amino acids. The protein's molecular weight is 23.71 kDa and its isoelectric point is 10.41.{{cite web | url=https://web.expasy.org/compute_pi/ | title=ExPASy Compute pl/Mw tool | access-date=4 January 2018}} DUF4703 is positioned 22-206aa of the protein. Neither the gene or its protein has another known alias, but can be found with high affinity in several primate species.{{cite web | url=https://www.ncbi.nlm.nih.gov/protein/XP_001150253.1?report=genpept | title=NCBI p. troglodytes Protein C7orf61 | access-date=4 January 2018}}

The amino acid composition of C7orf61 consists of high frequencies in leucine, serine, and charged valine.{{cite web | url=https://www.ebi.ac.uk/Tools/seqstats/saps/ | title=EMBL-EBI SAP | access-date=24 April 2018}} The protein has an unusual low frequency in asparagine, making it an asparagine-deficient protein, and contains higher frequencies of salt-bridge formations between glutamic acid, aspartic acid, lysine, and arginine.

The consent within secondary structure prediction tools CFSSP,{{cite web | url=http://www.biogem.org/tool/chou-fasman/index.php | title=CFSSP| access-date=23 April 2018}} SSPRED,{{cite web | url=http://linux1.softberry.com/berry.phtml?topic=sspred&group=programs&subgroup=propt | title=SoftBerry SSPRED| access-date=23 April 2018}} and GOR4 {{cite web | url=https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html | title=GOR4| access-date=23 April 2018}} is that the protein's secondary structure consist mainly of α-helices (51.2%), with significant amounts of coiling (38.2%) and smaller fragments of beta strands (10.3%).

File:C7orf61 protein.png

C7orf61 has several post-translation modification sites, most of which involve serine/threonine kinases - protein kinase C, Casein kinase II, DNA-dependent protein kinase, and Cyclin-dependent kinase 1. It is predicted to contain a Biparte nuclear localization signal (NLS_BP), a leucine-rich variant domain (LRV), and bacterial Ig-like domain (BIG-1).{{cite web | url=https://myhits.isb-sib.ch/cgi-bin/motif_scan | title=SIB Motif Scan | access-date=23 April 2018}}

C7orf61 does not contain any trans-membrane domains or signal peptides.{{cite web | url=http://www.cbs.dtu.dk/services/SignalP/ | title=SignalP 4.1 Server prediction tool| access-date=20 April 2018}}{{cite web | url=https://embnet.vital-it.ch/software/TMPRED_form.html | title=TMpred tool | access-date=21 April 2018 | archive-date=5 March 2019 | archive-url=https://web.archive.org/web/20190305234532/https://embnet.vital-it.ch/software/TMPRED_form.html | url-status=dead }} The protein is predicted to be localized in the Mitochondria, with little indication of extracellular activity.{{cite web | url=http://www.cbs.dtu.dk/services/DeepLoc/ | title=DeepLoc-1.0| access-date=24 April 2018}}{{cite web | url=http://www.softberry.com/berry.phtml?topic=protcompan&group=programs&subgroup=proloc | title=ProtComp 9.0| access-date=24 April 2018}} Expanded analysis of amino acid sequence KFFRWVRRAWQRIISWVF near the N-terminal suggests the presence of a mitochondrial targeting signal.{{cite web | url=http://www-nmr.cabm.rutgers.edu/bioinformatics/Proteomic_tools/Helical_wheel/ | title=Helical Wheel | access-date=25 April 2018 | archive-date=15 July 2019 | archive-url=https://web.archive.org/web/20190715002250/http://www-nmr.cabm.rutgers.edu/bioinformatics/Proteomic_tools/Helical_wheel/ | url-status=dead }}

C7orf61 has high levels of expression in the testis and lower levels in the brain and connective tissues.{{cite web | url=https://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.632306 | title=NCBI EST Profile - C7orf61| access-date=1 April 2018}} Through the assessment of microarray experiments available on NCBI Geo, its inferred that c7orf61 is under negative regulation.{{cite web | url=https://www.ncbi.nlm.nih.gov/geoprofiles/?term=c7orf61 | title=NCBI Geo| access-date=2 April 2018}}

C7orf61 does not have any paralogs. Analysis via NCBI tool BLASTt{{cite web | url=https://blast.ncbi.nlm.nih.gov/Blast.cgi | title=NCBI BLAST| access-date=4 February 2018}} found the gene to be highly conserved in mammals and could not be traced farther back than 160 MYA. The following table contains a list of orthologs found in several mammalian sub-classes - this is not a comprehensive list for the proteins orthology.

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