CCDC82

CCDC82 is located on chromosome 11 at 11q21.5.{{cite web |url = https://www.genecards.org/cgi-bin/carddisp.pl?gene=CCDC82&search=ccdc82#genomic_location |title = CCDC82 Genomic Views |author= |date= |website = GeneCards |publisher = Weizmann Institute of Science|access-date=}}

It contains two domains of unknown function, DUF4196 and DUF4211.{{cite web |url = https://www.genecards.org/cgi-bin/carddisp.pl?gene=CCDC82&search=ccdc82#domains_families |title =CCDC82 Protein Domains and Families |author= |date= |website = GeneCards |publisher = Weizmann Institute of Science|access-date=}} The DNA sequence is 37,155 base pairs long{{cite web|title=Homo sapiens chromosome 11, GRCh37.p10 Primary Assembly|date = 13 August 2013|url=https://www.ncbi.nlm.nih.gov/nuccore/NC_000011.9?from=96085929&to=96123083&report=genbank&strand=true|publisher=NCBI}} and contains 7 exons.{{cite web|title=Prediction of several variants of multiple genes|url=http://linux1.softberry.com/cgi-bin/programs/gfind/fgenes-m.pl|publisher=Softberry}}

CCDC82 is present in many orthologs. It is conserved throughout other mammals, reptiles, birds and bony fish. It is not found in invertebrates, bacteria or fungi. There are no paralogs.{{cite web|title=CCDC82 BLAST|url=http://blast.ncbi.nlm.nih.gov/Blast.cgi|work=BLAST|publisher=NCBI}}

File:CCDC82 Unrooted Phylogenetic Tree.png

The predicted promoter for CCDC82 is located on the minus strand and spans from base pairs 96,122,963 to 96,123,587. It is 625 base pairs long.{{cite web|title=Genome Browser|url=http://www.genomatix.de/cgi-bin//eldorado/eldorado.pl?s=5d2a4b7c0c9df5ca979324066717225b;SVG=CCDC82_1;ELDORADO_VERSION=E26R1204|work=ElDorado|publisher=Genomatix}}

The transcription factors listed below are for the predicted promoter sequence and are located on the minus strand.{{cite web|title=Transcription Factor Binding Sites|url=http://www.genomatix.de/cgi-bin//eldorado/eldorado.pl?s=f28839ced9048a0a83172c8a7737a93d;PROM_ID=GXP_3164115;GROUP=vertebrates;GROUP=others;ELDORADO_VERSION=E26R1204|work=ElDorado|publisher=Genomatix}}

The protein it encodes for is 344 amino acids in length. The protein itself is very acidic and is very rich in aspartic acid and glutamic acid. It is also very deficient in alanine, containing only two alanines in the entire sequence. The alanines are located adjacent to each other, amino acid number 233 and 234. Alanine 233 is highly conserved throughout the orthologs. The molecular weight is 40.0 kdal and the isoelectric point is 4.383{{cite web|title=SAPS |url=http://seqtool.sdsc.edu/CGI/BW.cgi#! |work=Biology Workbench |publisher=SDSU }}{{dead link|date=November 2016 |bot=InternetArchiveBot |fix-attempted=yes }}

File:CCDC82ExpressionData.png

CCDC82 is found in nearly all tissues in the human body, however it is present in higher quantities in the skeletal muscles, adrenal cortex, and the trigeminal ganglion.{{cite web|title=Large-scale analysis of the human transcriptome (HG-U133A)|url=https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS596:220693_at|work=GeoProfiles|publisher=NCBI}}{{cite journal |vauthors=Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL | title = Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 97 | issue = 17 | pages = 9543–8 |date=August 2000 | pmid = 10931946 | pmc = 16901 | doi = 10.1073/pnas.160270997 | bibcode = 2000PNAS...97.9543H | doi-access = free }}

CCDC82 has several predicted phosphorylation sites.{{cite journal |vauthors=Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M | title = Global, in vivo, and site-specific phosphorylation dynamics in signaling networks | journal = Cell | volume = 127 | issue = 3 | pages = 635–48 |date=November 2006 | pmid = 17081983 | doi = 10.1016/j.cell.2006.09.026 | s2cid = 7827573 | doi-access = free }} There are 32 predicted serine phosphorylation sites, 5 threonine, and 3 tyrosine.{{cite web|title=CCDC82|url=http://www.cbs.dtu.dk/cgi-bin/webface?jobid=netphos,5192A0E90509018D|work=NetPhos 2.0|publisher=Center for Biological Analysis|access-date=2013-05-14|archive-date=2013-10-16|archive-url=https://web.archive.org/web/20131016063712/http://www.cbs.dtu.dk/cgi-bin/webface?jobid=netphos,5192A0E90509018D|url-status=dead}}

CCDC82 is known to interact with two proteins. It indirectly interacts with VHL, a gene that encodes for a tumor suppressor and ubiquitin protein ligase. It also interacts with EWSR1, which functions as a transcriptional repressor.{{cite web|title=CCDC82|url=https://www.genecards.org/cgi-bin/carddisp.pl?gene=CCDC82&search=ccdc82#pathways_interactions|work=Gene Cards|publisher=Weizmann Institute of Science|accessdate=2 May 2013}}

CCDC82 is a circulat-responsive gene.{{cite journal |vauthors=Antoshechkin A, Olalde J, Magarici M, Muhammad A, Salom A, Suarez J, Amendola F | title = Analysis of effects of the herbal preparation Circulat on gene expression levels in cultured human fibroblasts | journal = Phytother Res | volume = 21 | issue = 8 | pages = 777–89 |date=August 2007 | pmid = 17514633 | doi = 10.1002/ptr.2174 | s2cid = 29862628 }} Circulat is a product designed to restore systemic vascular health. It is a plant based product and taken by patients who suffer from diabetes or circulatory problems.{{cite web|title=About Circulat|url=http://circulat.com/about-circulat|work=Circulat|publisher=Circulat Biotech LLC|url-status=dead|archiveurl=https://web.archive.org/web/20130831200303/http://circulat.com/about-circulat|archivedate=2013-08-31}}

Based on the information that CCDC82 is affected by the Circulat product it could be hypothesized that CCDC82 is involved in circulatory function. However, this is purely speculation.

{{Reflist}}

• {{UCSC gene info|CCDC82}}