CFP-10

{{Infobox nonhuman protein

| Name = 10 kDa culture filtrate antigen CFP-10

| image = CFP-10.jpg

| width =

| caption = Structure of the CFP10-ESAT6 complex from M. tuberculosis.{{PDB|1WA8}}; {{cite journal | vauthors = Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD | title = Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6 | journal = EMBO J. | volume = 24 | issue = 14 | pages = 2491–8 |date=July 2005 | pmid = 15973432 | pmc = 1176459 | doi = 10.1038/sj.emboj.7600732 }}

| Organism = Mycobacterium tuberculosis

| TaxID = 83332

| Symbol = esxB

| AltSymbols =

| IUPHAR_id =

| ATC_prefix =

| ATC_suffix =

| ATC_supplemental =

| CAS_number =

| CAS_supplemental =

| DrugBank =

| EntrezGene = 886194

| PDB = 3FAV

| RefSeqmRNA =

| RefSeqProtein = NP_218391

| UniProt = P0A566

| ECnumber =

| Chromosome = genome

| EntrezChromosome = NC_000962.2

| GenLoc_start = 4352228

| GenLoc_end = 4352620

}}

CFP-10 within bacterial proteins (also known as ESAT-6-like protein esxB or secreted antigenic protein MTSA-10 or 10 kDa culture filtrate antigen CFP-10) is a protein that is encoded by the esxB gene.{{cite web|url=https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=886194&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum|title=Entrez gene}}

CFP-10 is a 10 kDa secreted antigen from Mycobacterium tuberculosis. It forms a 1:1 heterodimeric complex with ESAT-6. Both genes are expressed from the RD1 region of the bacterial genome and play a key role in the virulence of the infection.{{cite journal |vauthors=Meher AK, Bal NC, Chary KV, Arora A |title=Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 compleand biochemical stability |journal=FEBS J. |volume=273 |issue=7 |pages=1445–62 |date=Apr 2006 |pmid=16689931 |doi=10.1111/j.1742-4658.2006.05166.x |doi-access=free }}

Function

10-kDa culture filtrate protein (CFP-10) is an antigen that contributes to the virulence Mycobacterium tuberculosis. CFP-10 forms a tight 1:1 heterodimeric complex with 6kDaA early secreted antigen target (ESAT-6). In the mycobacterial cell, these two proteins are interdependent on each other for stability. The ESAT-6/CFP-10 complex is secreted by the ESX-1 secretion system, also known as the RD1 region. Mycobacterium tuberculosis uses this ESX-1 secretion system to deliver virulence factors into host macrophage and monocyte white blood cells during infection.

In Mycobacterium tuberculosis, the core components of the whole ESX-1 secretion system include Rv3877, and two AAA ATPases, including Rv3870 and Rv3871, a cytosolic protein. The ESAT-6/CFP-10 heterodimer complex is targeted for secretion by a C-terminal signal sequence on CFP-10 that is recognized by the cytosolic Rv3871 protein. Rv3871 then interacts with the CFP-10 C-terminal, and escorts the ESAT-6/CFP-10 complex to Rv3870 and Rv3877, a multi-transmembrane protein which makes up the pore that spans the cytosolic membrane of the virulent host cell. Once ESAT-6/CFP-10 is next to the membrane of the virulent host cell, the CFP-10 C-terminal attaches and binds itself to the cells surface. The ESAT-6/CFP-10 complex’s secretion and attachment to the virulent host cell shows its contribution to the pathogenicity of Mycobacterium tuberculosis. ^[4].

CFP-10

Function

Structure

See also

References

Further reading