CHAF1B

Chromatin assembly factor I (CAF-1) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

CHAF1B has been shown to interact with:

• ASF1A,
• ASF1B,{{cite journal | vauthors = Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, Almouzni G | title = Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway | journal = EMBO Reports | volume = 3 | issue = 4 | pages = 329–34 | date = Apr 2002 | pmid = 11897662 | pmc = 1084056 | doi = 10.1093/embo-reports/kvf068 }}{{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | title = Large-scale mapping of human protein-protein interactions by mass spectrometry | journal = Molecular Systems Biology | volume = 3 | pages = 89 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 | year=2007}} and
• BAZ1B.{{cite journal | vauthors = Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, Matsui D, Ogawa S, Unno K, Okubo M, Tokita A, Nakagawa T, Ito T, Ishimi Y, Nagasawa H, Matsumoto T, Yanagisawa J, Kato S | title = The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome | journal = Cell | volume = 113 | issue = 7 | pages = 905–17 | date = Jun 2003 | pmid = 12837248 | doi = 10.1016/s0092-8674(03)00436-7| doi-access = free }}

{{reflist}}

{{refbegin | 2}}

• {{cite journal | vauthors = Ridgway P, Almouzni G | title = CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair | journal = Journal of Cell Science | volume = 113 | issue = 15 | pages = 2647–58 | date = Aug 2000 | doi = 10.1242/jcs.113.15.2647 | pmid = 10893180 | doi-access = free }}
• {{cite journal | vauthors = Blouin JL, Duriaux-Sail G, Chen H, Gos A, Morris MA, Rossier C, Antonarakis SE | title = Mapping of the gene for the p60 subunit of the human chromatin assembly factor (CAF1A) to the Down syndrome region of chromosome 21 | journal = Genomics | volume = 33 | issue = 2 | pages = 309–12 | date = Apr 1996 | pmid = 8660983 | doi = 10.1006/geno.1996.0199 }}
• {{cite journal | vauthors = Verreault A, Kaufman PD, Kobayashi R, Stillman B | title = Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4 | journal = Cell | volume = 87 | issue = 1 | pages = 95–104 | date = Oct 1996 | pmid = 8858152 | doi = 10.1016/S0092-8674(00)81326-4 | s2cid = 1416453 | doi-access = free }}
• {{cite journal | vauthors = Matsumoto-Taniura N, Pirollet F, Monroe R, Gerace L, Westendorf JM | title = Identification of novel M phase phosphoproteins by expression cloning | journal = Molecular Biology of the Cell | volume = 7 | issue = 9 | pages = 1455–69 | date = Sep 1996 | pmid = 8885239 | pmc = 275994 | doi = 10.1091/mbc.7.9.1455 }}
• {{cite journal | vauthors = Marheineke K, Krude T | title = Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle | journal = The Journal of Biological Chemistry | volume = 273 | issue = 24 | pages = 15279–86 | date = Jun 1998 | pmid = 9614144 | doi = 10.1074/jbc.273.24.15279 | doi-access = free }}
• {{cite journal | vauthors = Martini E, Roche DM, Marheineke K, Verreault A, Almouzni G | title = Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells | journal = The Journal of Cell Biology | volume = 143 | issue = 3 | pages = 563–75 | date = Nov 1998 | pmid = 9813080 | pmc = 2148138 | doi = 10.1083/jcb.143.3.563 }}
• {{cite journal | vauthors = Hattori M, Fujiyama A, Taylor TD, Watanabe H, Yada T, Park HS, Toyoda A, Ishii K, Totoki Y, Choi DK, Groner Y, Soeda E, Ohki M, Takagi T, Sakaki Y, Taudien S, Blechschmidt K, Polley A, Menzel U, Delabar J, Kumpf K, Lehmann R, Patterson D, Reichwald K, Rump A, Schillhabel M, Schudy A, Zimmermann W, Rosenthal A, Kudoh J, Schibuya K, Kawasaki K, Asakawa S, Shintani A, Sasaki T, Nagamine K, Mitsuyama S, Antonarakis SE, Minoshima S, Shimizu N, Nordsiek G, Hornischer K, Brant P, Scharfe M, Schon O, Desario A, Reichelt J, Kauer G, Blocker H, Ramser J, Beck A, Klages S, Hennig S, Riesselmann L, Dagand E, Haaf T, Wehrmeyer S, Borzym K, Gardiner K, Nizetic D, Francis F, Lehrach H, Reinhardt R, Yaspo ML | title = The DNA sequence of human chromosome 21 | journal = Nature | volume = 405 | issue = 6784 | pages = 311–9 | date = May 2000 | pmid = 10830953 | doi = 10.1038/35012518 | bibcode = 2000Natur.405..311H | doi-access = free }}
• {{cite journal | vauthors = Keller C, Krude T | title = Requirement of Cyclin/Cdk2 and protein phosphatase 1 activity for chromatin assembly factor 1-dependent chromatin assembly during DNA synthesis | journal = The Journal of Biological Chemistry | volume = 275 | issue = 45 | pages = 35512–21 | date = Nov 2000 | pmid = 10938080 | doi = 10.1074/jbc.M003073200 | doi-access = free }}
• {{cite journal | vauthors = Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, Almouzni G | title = Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway | journal = EMBO Reports | volume = 3 | issue = 4 | pages = 329–34 | date = Apr 2002 | pmid = 11897662 | pmc = 1084056 | doi = 10.1093/embo-reports/kvf068 }}
• {{cite journal | vauthors = Morel AP, Sentis S, Bianchin C, Le Romancer M, Jonard L, Rostan MC, Rimokh R, Corbo L | title = BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms | journal = Journal of Cell Science | volume = 116 | issue = Pt 14 | pages = 2929–36 | date = Jul 2003 | pmid = 12771185 | doi = 10.1242/jcs.00480 | doi-access = free }}
• {{cite journal | vauthors = Nabatiyan A, Krude T | title = Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis | journal = Molecular and Cellular Biology | volume = 24 | issue = 7 | pages = 2853–62 | date = Apr 2004 | pmid = 15024074 | pmc = 371118 | doi = 10.1128/MCB.24.7.2853-2862.2004 }}
• {{cite book | vauthors = Shim KS, Bergelson JM, Furuse M, Ovod V, Krude T, Lubec G | chapter = Reduction of chromatin assembly factor 1 p60 and C21orf2 protein, encoded on chromosome 21, in Down Syndrome brain | title = Advances in Down Syndrome Research | journal = Journal of Neural Transmission. Supplementum | volume = 67| issue = 67 | pages = 117–28 | pmid = 15068244 | doi = 10.1007/978-3-7091-6721-2_10 | series = Journal of Neural Transmission Supplement 67 | date = 2003 | isbn = 978-3-211-40776-9 }}
• {{cite journal | vauthors = Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP | title = Large-scale characterization of HeLa cell nuclear phosphoproteins | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 101 | issue = 33 | pages = 12130–5 | date = Aug 2004 | pmid = 15302935 | pmc = 514446 | doi = 10.1073/pnas.0404720101 | bibcode = 2004PNAS..10112130B | doi-access = free }}
• {{cite journal | vauthors = Lin KT, Lu RM, Tarn WY | title = The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo | journal = Molecular and Cellular Biology | volume = 24 | issue = 20 | pages = 9176–85 | date = Oct 2004 | pmid = 15456888 | pmc = 517884 | doi = 10.1128/MCB.24.20.9176-9185.2004 }}
• {{cite journal | vauthors = Groth A, Ray-Gallet D, Quivy JP, Lukas J, Bartek J, Almouzni G | title = Human Asf1 regulates the flow of S phase histones during replicational stress | journal = Molecular Cell | volume = 17 | issue = 2 | pages = 301–11 | date = Jan 2005 | pmid = 15664198 | doi = 10.1016/j.molcel.2004.12.018 | doi-access = free }}

{{refend}}

• {{UCSC gene info|CHAF1B}}
• {{UCSC gene info|MPP7}}

{{gene-21-stub}}