COMPLEAT (Bioinformatics tool)
{{Short description|Online bioinformatics tool}}
Protein Complex Enrichment Analysis Tool is an online bioinformatics tool used to analyze high-throughput datasets (or small-scale datasets) using protein complex enrichment analysis.{{cite journal|last=Vinayagam|first=A|author2=Hu, Y |author3=Kulkarni, M |author4=Roesel, C |author5=Sopko, R |author6=Mohr, SE |author7= Perrimon, N |title=Protein complex-based analysis framework for high-throughput data sets.|journal=Science Signaling|date=Feb 26, 2013|volume=6|issue=264|pages=rs5|pmid=23443684|doi=10.1126/scisignal.2003629 |pmc=3756668}} The tool uses a protein complex resource as the back end annotation data instead of conventional gene ontology- or pathway-based annotations. The tool incorporates several useful features in order to provide a comprehensive data-mining environment, including network-based visualization and interactive querying options.
COMPLEAT may be used to analyze RNAi screens, proteomic datasets, gene expression data and any other high-throughput datasets where protein complex information is relevant.
Applications
COMPLEAT has been successfully applied to identify:
- Dynamic protein complexes regulated by insulin and epidermal growth factors signaling, including a role of Brahma complex in the cellular response to insulin.
- Evolutionarily conserved molecular complexes that regulate nucleolar size when the complex constituents were targeted by RNA interference.{{cite journal|last=Neumüller|first=R.A |author2=Gross, T |author3=Samsonova, A |author4=Vinayagam, A |author5=Buckner, M |author6=Founk, K |author7=Hu, Y |author8=Sharifpoor, S |author9=Rosebrock, AP |author10=Andrews, B |author11=Winston, F |author12=Perrimon, N|title= Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses.|journal=Science Signaling|date=Aug 20, 2013|volume=6|issue=289|pages=ra70 |pmid=23962978|doi=10.1126/scisignal.2004145 |pmc=3964804}}
- Novel role of endocytosis and vesicle trafficking complexes in Hippo Signaling Pathway.{{cite journal|last=Kwon|first=Y|author2=Vinayagam, A |author3=Sun, X |author4=Dephoure, N |author5=Gygi, S.P |author6=Hong, P |author7= Perrimon, N |title= The Hippo Signaling Pathway Interactome.|journal=Science |year=2013 |doi=10.1126/science.1243971 |pmid=24114784 |volume=342 |issue=6159 |pages=737–40 |pmc=3951131|bibcode=2013Sci...342..737K}}
References
{{Reflist}}
External links
- [http://www.flyrnai.org/compleat/ COMPLEAT]