Cholate—CoA ligase

{{Infobox enzyme

| Name = Cholate—CoA ligase

| EC_number = 6.2.1.7

| CAS_number = 9027-90-1

| GO_code =

| image =

| width =

| caption =

}}

Cholate—CoA ligase ({{EnzExplorer|6.2.1.7}}, BAL, bile acid CoA ligase, bile acid coenzyme A ligase, choloyl-CoA synthetase, choloyl coenzyme A synthetase, cholic thiokinase, cholate thiokinase, cholic acid:CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase, THCA-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate—CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming), cholyl-CoA synthetase, trihydroxycoprostanoyl-CoA synthetase) is an enzyme with systematic name cholate:CoA ligase (AMP-forming).{{cite journal | vauthors = Elliott WH | title = The enzymic activation of cholic acid by guinea-pig-liver microsomes | journal = The Biochemical Journal | volume = 62 | issue = 3 | pages = 427–33 | date = March 1956 | pmid = 13303991 | pmc = 1215932 }}{{cite journal | vauthors = Elliott WH | title = The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes | journal = The Biochemical Journal | volume = 65 | issue = 2 | pages = 315–21 | date = February 1957 | pmid = 13403911 | pmc = 1199872 }}{{cite journal | vauthors = Prydz K, Kase BF, Björkhem I, Pedersen JI | title = Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver | journal = Journal of Lipid Research | volume = 29 | issue = 8 | pages = 997–1004 | date = August 1988 | pmid = 3183523 }}{{cite journal | vauthors = Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP | title = Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver | journal = The Biochemical Journal | volume = 257 | issue = 1 | pages = 221–9 | date = January 1989 | pmid = 2521999 | pmc = 1135559 }}{{cite journal | vauthors = Mallonee DH, Adams JL, Hylemon PB | title = The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase | journal = Journal of Bacteriology | volume = 174 | issue = 7 | pages = 2065–71 | date = April 1992 | pmid = 1551828 | pmc = 205821 }}{{cite journal | vauthors = Wheeler JB, Shaw DR, Barnes S | title = Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes | journal = Archives of Biochemistry and Biophysics | volume = 348 | issue = 1 | pages = 15–24 | date = December 1997 | pmid = 9390170 | doi = 10.1006/abbi.1997.0391 }}{{cite journal | vauthors = Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S | title = Molecular cloning and expression of rat liver bile acid CoA ligase | journal = Journal of Lipid Research | volume = 43 | issue = 12 | pages = 2062–71 | date = December 2002 | pmid = 12454267 | doi = 10.1194/jlr.M200260-JLR200 | doi-access = free }} This enzyme catalyses the following chemical reaction

: (1) ATP + cholate + CoA $\backslash rightleftharpoons$ AMP + diphosphate + choloyl-CoA

: (2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA $\backslash rightleftharpoons$ AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA

This enzyme requires Mg²⁺ for activity.