Cofactor of BRCA1

NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2; MIM 606026), either NELF-C or NELF-D (TH1L; MIM 605297), and NELF-E (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660).{{cite journal | vauthors = Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H | title = Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex | journal = Molecular and Cellular Biology | volume = 23 | issue = 6 | pages = 1863–1873 | year = 2003 | pmid = 12612062 | pmc = 149481 | doi = 10.1128/MCB.23.6.1863-1873.2003 }} COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.{{cite journal | vauthors = Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R | title = BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations | journal = The Journal of Cell Biology | volume = 155 | issue = 6 | pages = 911–922 | year = 2001 | pmid = 11739404 | pmc = 2150890 | doi = 10.1083/jcb.200108049 }}

Cofactor of BRCA1 has been shown to interact with:

• BRCA1{{cite journal | vauthors = Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R | title = BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations | journal = J. Cell Biol. | volume = 155 | issue = 6 | pages = 911–921 | date = Dec 2001 | pmid = 11739404 | pmc = 2150890 | doi = 10.1083/jcb.200108049 }}
• C-Fos,
• C-jun,{{cite journal | vauthors = Zhong H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Q | title = COBRA1 inhibits AP-1 transcriptional activity in transfected cells | journal = Biochem. Biophys. Res. Commun. | volume = 325 | issue = 2 | pages = 568–573 | date = Dec 2004 | pmid = 15530430 | doi = 10.1016/j.bbrc.2004.10.079 }}
• Estrogen receptor alpha,{{cite journal | vauthors = Aiyar SE, Sun JL, Blair AL, Moskaluk CA, Lu YZ, Ye QN, Yamaguchi Y, Mukherjee A, Ren DM, Handa H, Li R | title = Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor | journal = Genes Dev. | volume = 18 | issue = 17 | pages = 2134–2146 | date = Sep 2004 | pmid = 15342491 | pmc = 515291 | doi = 10.1101/gad.1214104 }}
• RDBP,{{cite journal | vauthors = Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM | title = Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region | journal = Genomics | volume = 83 | issue = 1 | pages = 153–167 | date = Jan 2004 | pmid = 14667819 | doi = 10.1016/S0888-7543(03)00235-0}} and
• TH1L.{{cite journal | vauthors = Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H | title = Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex | journal = Mol. Cell. Biol. | volume = 23 | issue = 6 | pages = 1863–1873 | date = Mar 2003 | pmid = 12612062 | pmc = 149481 | doi = 10.1128/MCB.23.6.1863-1873.2003}}{{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | title = Large-scale mapping of human protein-protein interactions by mass spectrometry | journal = Mol. Syst. Biol. | volume = 3 | pages = 89 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 | year=2007}}

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