EIF3D

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.

EIF3D has been shown to interact with PHLDA1{{cite journal | vauthors = Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D | title = Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product | journal = Cellular Signalling | volume = 13 | issue = 5 | pages = 345–352 | date = May 2001 | pmid = 11369516 | doi = 10.1016/S0898-6568(01)00141-3 }} and EIF3A.{{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | display-authors = 6 | title = Large-scale mapping of human protein-protein interactions by mass spectrometry | journal = Molecular Systems Biology | volume = 3 | issue = 1 | pages = 89 | year = 2007 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 }}{{cite journal | vauthors = Mayeur GL, Fraser CS, Peiretti F, Block KL, Hershey JW | title = Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor elF3 | journal = European Journal of Biochemistry | volume = 270 | issue = 20 | pages = 4133–4139 | date = October 2003 | pmid = 14519125 | doi = 10.1046/j.1432-1033.2003.03807.x | doi-access = free }}{{cite journal | vauthors = Block KL, Vornlocher HP, Hershey JW | title = Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3 | journal = The Journal of Biological Chemistry | volume = 273 | issue = 48 | pages = 31901–31908 | date = November 1998 | pmid = 9822659 | doi = 10.1074/jbc.273.48.31901 | doi-access = free }}

EIF3D has also been shown to interact with c-Jun mRNA via a non-canonical mechanism. Instead of the EIF4G protein acting as a cap-binding protein to mediate translation, EIF3D has been shown to be a cap binding protein for certain mRNAs such as c-Jun which has structures at the 5' UTR inhibiting binding of EIF4G and promoting binding of EIF3D.{{cite journal | vauthors = Lee AS, Kranzusch PJ, Doudna JA, Cate JH | title = eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation | journal = Nature | volume = 536 | issue = 7614 | pages = 96–99 | date = August 2016 | pmid = 27462815 | pmc = 5003174 | doi = 10.1038/nature18954 | bibcode = 2016Natur.536...96L }} EIF3D as a cap binding protein has been thought of as critical to regulating gene expression under cell stress such as during glucose deprivation. For translation of c-Jun under glucose starved conditions, the cap binding activity of EIF3D increased by 10-fold.{{cite journal | vauthors = Jia L, Qian SB | title = A Versatile eIF3d in Translational Control of Stress Adaptation | language = English | journal = Molecular Cell | volume = 81 | issue = 1 | pages = 10–12 | date = January 2021 | pmid = 33417853 | doi = 10.1016/j.molcel.2020.12.016 | s2cid = 231303797 | doi-access = free }}{{cite journal | vauthors = Lamper AM, Fleming RH, Ladd KM, Lee AS | title = A phosphorylation-regulated eIF3d translation switch mediates cellular adaptation to metabolic stress | journal = Science | volume = 370 | issue = 6518 | pages = 853–856 | date = November 2020 | pmid = 33184215 | doi = 10.1126/science.abb0993 | bibcode = 2020Sci...370..853L | s2cid = 226308112 }}

• Eukaryotic initiation factor 3 (eIF3)

{{reflist}}

{{refbegin | 2}}

• {{cite journal | vauthors = Asano K, Kinzy TG, Merrick WC, Hershey JW | title = Conservation and diversity of eukaryotic translation initiation factor eIF3 | journal = The Journal of Biological Chemistry | volume = 272 | issue = 2 | pages = 1101–1109 | date = January 1997 | pmid = 8995409 | doi = 10.1074/jbc.272.2.1101 | doi-access = free }}
• {{cite journal | vauthors = Méthot N, Rom E, Olsen H, Sonenberg N | title = The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170 | journal = The Journal of Biological Chemistry | volume = 272 | issue = 2 | pages = 1110–1116 | date = January 1997 | pmid = 8995410 | doi = 10.1074/jbc.272.2.1110 | doi-access = free }}
• {{cite journal | vauthors = Block KL, Vornlocher HP, Hershey JW | title = Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3 | journal = The Journal of Biological Chemistry | volume = 273 | issue = 48 | pages = 31901–31908 | date = November 1998 | pmid = 9822659 | doi = 10.1074/jbc.273.48.31901 | doi-access = free }}
• {{cite journal | vauthors = Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP | display-authors = 6 | title = The DNA sequence of human chromosome 22 | journal = Nature | volume = 402 | issue = 6761 | pages = 489–495 | date = December 1999 | pmid = 10591208 | doi = 10.1038/990031 | bibcode = 1999Natur.402..489D | doi-access = free }}
• {{cite journal | vauthors = Asano K, Shalev A, Phan L, Nielsen K, Clayton J, Valásek L, Donahue TF, Hinnebusch AG | display-authors = 6 | title = Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation | journal = The EMBO Journal | volume = 20 | issue = 9 | pages = 2326–2337 | date = May 2001 | pmid = 11331597 | pmc = 125443 | doi = 10.1093/emboj/20.9.2326 }}
• {{cite journal | vauthors = Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D | title = Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product | journal = Cellular Signalling | volume = 13 | issue = 5 | pages = 345–352 | date = May 2001 | pmid = 11369516 | doi = 10.1016/S0898-6568(01)00141-3 }}
• {{cite journal | vauthors = Morris-Desbois C, Réty S, Ferro M, Garin J, Jalinot P | title = The human protein HSPC021 interacts with Int-6 and is associated with eukaryotic translation initiation factor 3 | journal = The Journal of Biological Chemistry | volume = 276 | issue = 49 | pages = 45988–45995 | date = December 2001 | pmid = 11590142 | doi = 10.1074/jbc.M104966200 | doi-access = free }}
• {{cite journal | vauthors = Mayeur GL, Fraser CS, Peiretti F, Block KL, Hershey JW | title = Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor elF3 | journal = European Journal of Biochemistry | volume = 270 | issue = 20 | pages = 4133–4139 | date = October 2003 | pmid = 14519125 | doi = 10.1046/j.1432-1033.2003.03807.x | doi-access = free }}
• {{cite journal | vauthors = Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I | display-authors = 6 | title = A genome annotation-driven approach to cloning the human ORFeome | journal = Genome Biology | volume = 5 | issue = 10 | pages = R84 | year = 2004 | pmid = 15461802 | pmc = 545604 | doi = 10.1186/gb-2004-5-10-r84 | doi-access = free }}
• {{cite journal | vauthors = Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ | display-authors = 6 | title = Immunoaffinity profiling of tyrosine phosphorylation in cancer cells | journal = Nature Biotechnology | volume = 23 | issue = 1 | pages = 94–101 | date = January 2005 | pmid = 15592455 | doi = 10.1038/nbt1046 | s2cid = 7200157 }}
• {{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | display-authors = 6 | title = Large-scale mapping of human protein-protein interactions by mass spectrometry | journal = Molecular Systems Biology | volume = 3 | issue = 1 | pages = 89 | year = 2007 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 }}

{{refend}}

{{Initiation factors}}

{{gene-22-stub}}