EP-B2
Endoprotease B, isoform 2 (EP-B2) is a cysteine protease found in barley. In studies, it has shown promise as a possible therapeutic for coeliac disease.{{Cite journal
| volume = 98
| journal = Biotechnology and Bioengineering
| title = A scaleable manufacturing process for pro-EP-B2, a cysteine protease from barley indicated for celiac sprue
| issue = 1
| pages = 177–185
| doi = 10.1002/bit.21423
| pmid = 17385743
| year = 2007 | first5 = C.
| last5 = Khosla | first2 = J.
| last2 = McIntire | first1 = H.
| last3 = Kumar | first3 = P. | first4 = M.
| last4 = Deshpande
| last1 = Vora
| s2cid = 19581223
}} Aspergillopepsin from Aspergillus niger has been shown to enhance the effects of EP-B2.{{Cite journal
| last1 = Ehren | first1 = J.
| last2 = Morón | first2 = B.
| last3 = Martin | first3 = E.
| last4 = Bethune | first4 = M.
| last5 = Gray | first5 = G.
| last6 = Khosla | first6 = C.
| last7 = El-Shemy | first7 = H. A.
| title = A food-grade enzyme preparation with modest gluten detoxification properties
| journal = PLOS ONE
| volume = 4
| issue = 7
| pages = e6313
| year = 2009
| pmid = 19621078
| pmc = 2708912
| doi = 10.1371/journal.pone.0006313
|bibcode = 2009PLoSO...4.6313E | doi-access = free
}}
References
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Further reading
{{refbegin}}
- {{Cite journal
| last1 = Bethune | first1 = M.
| last2 = Ribka | first2 = E.
| last3 = Khosla | first3 = C.
| last4 = Sestak | first4 = K.
| last5 = Bozza | first5 = P.
| title = Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques
| journal = PLOS ONE
| volume = 3
| issue = 3
| pages = e1857
| year = 2008
| pmid = 18365012
| pmc = 2267209
| doi = 10.1371/journal.pone.0001857
|bibcode = 2008PLoSO...3.1857B | doi-access = free
}}
- {{Cite journal
| last1 = Gass | first1 = J.
| last2 = Bethune | first2 = M.
| last3 = Siegel | first3 = M.
| last4 = Spencer | first4 = A.
| last5 = Khosla | first5 = C.
| title = Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue
| journal = Gastroenterology
| volume = 133
| issue = 2
| pages = 472–480
| year = 2007
| pmid = 17681168
| doi = 10.1053/j.gastro.2007.05.028
| doi-access = free
}}
- {{Cite journal | first9 = P. | last10 = Khosla | first10 = C.
| title = Rational design of combination enzyme therapy for celiac sprue
| last9 = Lee | first8 = G.
| last8 = Gray
| journal = Chemistry & Biology
| volume = 13
| pmid = 16793522
| doi = 10.1016/j.chembiol.2006.04.009
| year = 2006
| pages = 649–658
| issue = 6 | first7 = T.
| last7 = Stuge
| last3 = Gass | first3 = J. | first2 = M.
| last2 = Bethune | first1 = M.
| last4 = Ehren | first4 = J. | first6 = A.
| last6 = Johannsen | first5 = J.
| last5 = Xia
| last1 = Siegel
| doi-access = free
}}
- {{Cite journal
| volume = 13
| journal = Chemistry & Biology
| title = Heterologous expression, purification, refolding, and structural-functional characterization of EP-B2, a self-activating barley cysteine endoprotease
| issue = 6
| pages = 637–647
| doi = 10.1016/j.chembiol.2006.04.008
| pmid = 16793521
| year = 2006 | first5 = C.
| last5 = Khosla | first2 = P.
| last2 = Strop | first1 = M.
| last3 = Tang | first3 = Y. | first4 = L.
| last4 = Sollid
| last1 = Bethune
| doi-access = free
}}
- {{Cite journal
| last1 = Gass | first1 = J.
| last2 = Vora | first2 = H.
| last3 = Bethune | first3 = M.
| last4 = Gray | first4 = G.
| last5 = Khosla | first5 = C.
| title = Effect of barley endoprotease EP-B2 on gluten digestion in the intact rat
| journal = The Journal of Pharmacology and Experimental Therapeutics
| volume = 318
| issue = 3
| pages = 1178–1186
| year = 2006
| pmid = 16757540
| doi = 10.1124/jpet.106.104315
| s2cid = 33662709
}}
{{refend}}
{{Cysteine proteases}}
{{Enzymes}}
{{Portal bar|Biology|border=no}}
{{enzyme-stub}}