Flavonoid 3′-monooxygenase

{{Short description|Class of enzymes}}

{{infobox enzyme

| Name = flavonoid 3′-monooxygenase

| EC_number = 1.14.14.82

| CAS_number = 85340-98-3

| GO_code = 0016711

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In enzymology, a flavonoid 3′-monooxygenase ({{EC number|1.14.14.82}}, was wrongly classified as {{EC number|1.14.13.21}} in the past) is an enzyme that catalyzes the chemical reaction{{cite journal | vauthors =Forkmann G, Heller W, Grisebach H |title = Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3′-hydroxylases |journal = Z. Naturforsch. C: Biosci. |volume = 35 |year = 1980 |pages = 691–695}}

{{block indent|a flavonoid + NADPH + H⁺ + O₂ $\backslash rightleftharpoons$ a 3′-hydroxyflavonoid + NADP⁺ + H₂O}}

The 4 substrates of this enzyme are flavonoid, NADPH, H⁺, and O₂, whereas its 3 products are 3′-hydroxyflavonoid, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O₂ as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O₂ with NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is flavonoid,NADPH:oxygen oxidoreductase (3′-hydroxylating). Other names in common use include flavonoid 3′-hydroxylase, flavonoid 3-hydroxylase (erroneous), NADPH:flavonoid-3′-hydroxylase, and flavonoid 3-monooxygenase (erroneous). This enzyme participates in flavonoid biosynthesis.