Gambusia longispinis

{{Short description|Species of fish}}

{{Speciesbox

| image =

| status = EN | status_system = IUCN3.1

| status_ref = {{cite iucn |author=Lyons, T.J. |author2=Matamoros, W.A. |author3=Vega-Cendejas, M. |date=2019 |title=Gambusia longispinis |volume=2019 |page=e.T8894A3147805 |doi=10.2305/IUCN.UK.2019-2.RLTS.T8894A3147805.en |access-date=16 November 2021}}

| taxon = Gambusia longispinis

| authority = W. L. Minckley, 1962

| synonyms =

}}

Gambusia longispinis, the Cuatrociengas gambusia (also locally called the Guayacon de Cuatro Cienegas), is an endangered species of fish in the family Poeciliidae. It is endemic to Cuatro Ciénegas in Mexico.{{FishBase|Gambusia|longispinis|month=August|year-2019}}