H295R

{{Short description|Angiotensin-II-responsive steroid-producing adrenocortical cell line}}

H295R (also referred to as NCI-H295R) is an angiotensin-II-responsive steroid-producing adrenocortical cell line.{{cite journal |vauthors=Wang T, Rainey WE |title= Human Adrenocortical Carcinoma Cell Lines. |journal= Mol. Cell. Endocrinol. |volume= 351 |issue= 1 |pages= 58–65 |year= 2012 |pmid= 21924324 |doi= 10.1016/j.mce.2011.08.041 |pmc=3288152}} It was initially isolated in 1980 from a 48-year-old female patient diagnosed with adrenocortical carcinoma.{{cite journal |vauthors=Gazdar AF, Oie HK, Shackleton CH, Chen TR, Triche TJ, Myers CE, Chrousos GP, Brennan MF, Stein CA, La Rocca RV |title= Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. |journal= Cancer Res. |volume= 50 |issue= 17 |pages= 5488–5496 |year= 1990 |pmid= 2386954 |url= http://cancerres.aacrjournals.org/content/50/17/5488.long }} The initial polyclonal populations of tumor cells obtained from the patients' tumor were cultured and the resultant cell line was called NCI-H295. Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth. Three strains were developed, based on the serum supplement used for growth, which have been termed H295R-S1, H295R-S2 and H295R-S3.{{cite journal |vauthors=Rainey WE, Saner K, Schimmer BP |title= Adrenocortical cell lines. |journal= Mol. Cell. Endocrinol. |volume= 228 |issue= 1–2 |pages= 23–38 |year= 2004 |pmid= 15541570 |doi= 10.1016/j.mce.2003.12.020 |s2cid= 1628345 }} All three strains grow as adherent monolayer cultures.