HaCaT

HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin,{{cite journal |last1=Boukamp |first1=Petra |last2=Petrussevska |first2=Rule T. |last3=Breitkreutz |first3=Dirk |last4=Hornung |first4=Jiirgen |last5=Markham |first5=Alex |last6=Fusenig |first6=Norbert E. |title=Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line |journal=The Journal of Cell Biology |date=1 March 1988 |volume=106 |issue=3 |pages=761–771 |doi=10.1083/jcb.106.3.761 |pmid=2450098 |pmc=2115116 }}{{cite journal |last1=Ölschläger |first1=Veronika |last2=Schrader |first2=Andreas |last3=Hockertz |first3=Stefan |title=Comparison of Primary Human Fibroblasts and Keratinocytes with Immortalized Cell Lines Regarding their Sensitivity to Sodium Dodecyl Sulfate in a Neutral Red Uptake Cytotoxicity Assay |journal=Arzneimittelforschung |date=13 December 2011 |volume=59 |issue=3 |pages=146–152 |doi=10.1055/s-0031-1296378 |pmid=19402346 |s2cid=20840726 }} widely used in scientific research.{{cite journal |last1=Schoop |first1=Veronika M. |last2=Fusenig |first2=Norbert E. |last3=Mirancea |first3=Nicolae |title=Epidermal Organization and Differentiation of HaCaT Keratinocytes in Organotypic Coculture with Human Dermal Fibroblasts |journal=Journal of Investigative Dermatology |date=March 1999 |volume=112 |issue=3 |pages=343–353 |doi=10.1046/j.1523-1747.1999.00524.x |pmid=10084313 |doi-access=free }} HaCaT cells are utilized for their high capacity to differentiate and proliferate in vitro.{{cite journal|last=Schürer|first=N|author2=Köhne, A |author3=Schliep, V |author4=Barlag, K |author5=Goerz, G |title=Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.|journal=Experimental Dermatology|date=Aug 1993|volume=2|issue=4|pages=179–85|pmid=8162337 |doi=10.1111/j.1600-0625.1993.tb00030.x|s2cid=20709063}} Their use in research allows for the characterization of human keratinocyte using a model that is reproducible and addresses issues such as short culture lifespan and variations between cell lines that would otherwise be encountered. These cells have allowed the characterization of several processes, such as their utilization as a model system for vitamin D₃ metabolism in the skin.{{cite journal|last=Lehmann|first=Bodo|title=HaCaT Cell Line as a Model System for Vitamin D3 Metabolism in Human Skin.|journal=Journal of Investigative Dermatology|date=1 January 1997|volume=108|issue=1|pages=78–82|doi=10.1111/1523-1747.ep12285640|pmid=8980292|doi-access=free}}