Holtfreter's solution

{{Short description|Salt solution used in biology}}

Holtfreter's solution (Holtfreter's medium) is a balanced salt solution that was developed by the developmental biologist Johannes Holtfreter for studying amphibian embryos and to reduce bacterial infections.{{Cite web|url=http://www.nasonline.org/publications/biographical-memoirs/memoir-pdfs/holtfreter-johannes.pdf|title=Johannes Holtfreter, 1901—1992. A Biographical Memoir by John Gerhart|website=National Academy of Sciences (USA)}} As a specialised aqueous solution, it finds use in aquaria to prevent infections for early stage amphibians, where it is typically mixed with soft tap water.{{cite web|url=http://www.axolotl.org/requirements.htm#hardness |title=Axolotls - Requirements & Water Conditions in Captivity |publisher=Axolotl.org |date= |accessdate=2011-03-29}}{{cite web|url=http://www.ambystoma.org/AGSC/axolotls/shortguide/solutions.html |title=Indiana University Axolotl Colony |publisher=Ambystoma.org |date= |accessdate=2011-03-29}} Amphibians such as axolotls prefer a hard water solution.{{citation needed|date=March 2013}}

Composition

Molarity of the component salts are as follows:

class="wikitable"
NaCl0.059 M
KCl0.00067 M
CaCl20.00076 M
NaHCO30.0024 M

Notes

{{reflist}}

  • https://www.msu.edu/user/eisthen/lab/methods/animalcare/holtfr.html [link now dead]

References

  • Armstrong, J.B., Duhon, S.T., and Malacinski, G.M. (1989) "Raising the axolotl in captivity". In J. B. Armstrong and G. M. Malacinski (eds.) Developmental Biology of the Axolotl. New York: Oxford University Press, pp. 220–227.

Category:Solutions

Category:Antimicrobials

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