Lestremiinae

{{Short description|Subfamily of flies}}

{{Automatic taxobox

| taxon = Lestremiinae

| image_caption = Photo of an adult male Lestremia

| image = Lestremia_(Tamaki).jpg

| authority =

| subdivision_ranks = Genera

| subdivision =

}}

Lestremiinae is a subfamily of Cecidomyiidae. It is composed of 105 described species classified into 13 genera.{{Cite Q|Q109561625}} The larvae feed on fungi, primarily in rotting wood.