Ligation-independent cloning

Ligation-independent cloning (LIC) is a form of molecular cloning that can be performed without the use of restriction endonucleases or DNA ligase. The technique was developed in the early 1990s as an alternative to restriction enzyme/ligase cloning.{{Cite web|url=https://www.neb.com/applications/cloning-and-synthetic-biology/seamless-cloning|title=Seamless Cloning {{!}} NEB|website=www.neb.com|access-date=2020-01-23}} This allows genes to be cloned without the requirement of a restriction site for cloning that is absent from the gene insert.{{cite web|title=Ligation Independent Cloning (LIC) |publisher=New England BioLabs (NEB) |url=https://www.neb.com/applications/cloning-and-synthetic-biology/ligation-independent-cloning |accessdate=15 January 2016}}{{cite web|url=http://bitesizebio.com/2008/01/08/get-your-clone-90-of-the-time-with-ligation-independent-cloning/ |title=Get Your Clone 90% Of The Time with Ligation Independent Cloning |publisher=Bitesize Bio |date= |accessdate=2012-05-09}}{{cite journal |pmid=1362067 |year=1992 |last1=Haun |first1=RS |last2=Serventi |first2=IM |last3=Moss |first3=J |title=Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors |volume=13 |issue=4 |pages=515–8 |journal=BioTechniques}}{{clarify|date=October 2011}} LIC uses long complementary overhangs on the vector and the DNA insert to create a stable association between them. {{cite book |last1=Snyder |first1=Lori A. S. |title=Bacterial genetics and genomics |date=2020 |publisher=CRC Press |location=Boca Raton, FL |isbn=9781000039191 |page=301 |chapter=Laboratory Techniques}}