| class="wikitable sortable"
!width="200px"|Name
!Nickname / AKA!! width="100px" |Years active!!data-sort-type="number"|Proven victims!!data-sort-type="number"|Possible victims!!Status!!Notes!!Ref |
| Martin Roháč | | 1568–1571 | 59 | N/A | Executed | Soldier turned robber and serial killer with the alleged highest number of victims in the Czech lands. | [{{cite web |title=Martin Roháč - Popravy - Hrdelní právo |url=http://www.hrdelnipravo.cz/popravy/martin-rohac.html |website=hrdelnipravo.cz |access-date=1 August 2023 |language=cs}}] |
| Kateřina of Komárov | | 1529–1533 | 14 | 16 | Exiled | Noblewoman who was infamous for the rumours of her mistreatment of the serfs on the estates of her spouse, which she tended during his absences. She was brought to trial in 1533 and convicted for the murder of 14 people, though she was allegedly the murderer of 30 people. She was sentenced to imprisonment in a tower in Prague Castle. | [{{cite web |last=Apfel |first=Viliam |title=Ďábel v brokátu: Bestiální zločiny urozené Kateřiny z Komárova|url=https://www.stoplusjednicka.cz/dabel-v-brokatu-bestialni-zlociny-urozene-kateriny-z-komarova |website=100+1 zahraniční zajímavost|access-date=1 August 2023 |language=cs}}] |
| Jaroslav and Dana Stodolovi | | 2001–2002 | 8 | 2 | Life imprisonment | Pair of Czech-Slovak serial killers who robbed and murdered several retirees. Five people survived their attacks. In total, they were convicted of committing 17 serious crimes and both were sentenced to life imprisonment. | |
| Petr Zelenka | Heparin killer | 2006 | 7 | 14 | Life imprisonment | Male nurse in Havlíčkův Brod who murdered seven patients by lethal injection. | [{{cite web |title="Heparin killer" gets life in prison |url=http://www.radio.cz/en/section/curraffrs/heparin-killer-gets-life-in-prison |website=Radio Prague International |access-date=1 August 2023 |date=22 February 2008}}] |
| Václav Mrázek | Death | 1951–1956 | 7 | Several | Executed | Convicted of killing at least seven people. While primarily sexually-motivated, he also robbed his victims, and at trial, he was convicted of 127 total crimes. | [{{cite web |title=Václav Mrázek |url=https://www.serialkillers.cz/vaclav-mrazek |website=SerialKillers.cz |access-date=10 August 2023 |language=cs}}] |
| František Novotný | | 1888–1898 | 7 | 0 | Executed | Bought a farm from the widow of serial killer Josef Englich and moved in with his wife. He gradually poisoned six children who were born to them on the farm. Unexplained deaths eventually led to the divorce and Novotný remarried. He also poisoned the son from this marriage. The motive was his general hatred of children. | [{{cite web |last1=Mareš |first1=Jaroslav |title=Dům dvou sériových vrahů: Děsivý příběh vraha Novotného|url=https://badatele.net/dum-dvou-seriovych-vrahu-desivy-pribeh-vraha-novotneho/ |website=badatele.net |access-date=1 August 2023 |language=cs |date=29 October 2022}}] |
| Anton Schimak | Moravian Babinský | 1887 | 6 | 0 | Executed | A robber murderer who, after his release from Mírov Prison murdered and robbed six people and shot a Catholic priest in just a few days. | [{{cite web |title=Vrah Schimak: Zabil tolik lidí, že už je nikdo nespočítá. Udala ho hostinská, když se probouzel |url=https://www.securitymagazin.cz/security/vrah-schimak-zabil-tolik-lidi-ze-uz-je-nikdo-nespocita-pak-utekl-do-lesa-udala-ho-hostinska-kdyz-se-probouzel-1404058874.html |website=securitymagazin.cz |access-date=1 August 2023 |language=cs}}] |
Karel Kopáč
Ludvík Černý
Vladimír Kuna | Orlík killers | 1991–1993 | 5 | 0 | Kopáč died in prison
Černý: Life imprisonment
Kuna released in 2013 | Gang of serial killers who, between 1991 and 1993, killed five people (mostly businessmen) for monetary gain. One of the members committed a murder on his own and attempted another, but the victim in the latter attack survived. | |
| Hubert Pilčík | | 1948–1951 | 5 | 10+ | Executed | Acted as a conduit through the Iron Curtain who killed and robbed several people he was supposed to smuggle across the border. | [{{cite web |title=Oběti strýčka Huberta. Skutečnost vs. případ majora Zemana |url=http://www.rozhlas.cz/dvojka/historiezlocinu/_zprava/obeti-strycka-huberta--1582774 |website=Czech Radio |access-date=1 August 2023 |language=cs |date=10 August 2016}}] |
| Ivan Roubal | | 1991–1994 | 5 | 3 | Died in prison | Killed for selfish reasons. He was not charged in the three murders because no bodies were found. | |
| Ladislav Hojer | | 1978–1981 | 5 | 3 | Executed | Sexual murderer who committed murders during his trips around the country, making his capture much more difficult. He committed cannibalism on one of the victims. | [{{cite web |title=Ladislav Hojer |url=http://kriminalistika.eu/muzeumzla/hojer/hojer.html |website=kriminalistika.eu |access-date=1 August 2023|language=cs}}] |
| Oto Biederman | | 1993–1996 | 5 | 0 | Life imprisonment | Member of the "Kolín Gang", sentenced to life imprisonment for murdering 5 people. | [{{cite web |title=Případ Apollo aneb pět vražd Kolínského gangu |url=https://dvojka.rozhlas.cz/pripad-apollo-aneb-pet-vrazd-kolinskeho-gangu-6942784 |publisher=Czech Radio |access-date=1 August 2023 |language=cs |date=4 July 2018}}] |
| Josef Englich | | 1885 | 5 | 0 | Executed | Married on a farm in Komárov, where a widowed woman lived with her daughter, parents and grandparents. According to custom, he was supposed to pay them off and then house them in exchange and take care of them until they died. For financial reasons, he gradually poisoned all members of the household except his wife with arsenic. | [{{cite web |title=Dva vrazi a 12 mrtvých: Tohle je opravdový dům hrůzy! |url=https://www.ahaonline.cz/clanek/musite-vedet/202614/dva-vrazi-a-12-mrtvych-tohle-je-opravdovy-dum-hruzy.html |website=Ahaonline.cz |access-date=1 August 2023|language=cs}}] |
Ondrej Gažik
František Gaži
František Daniš | | 1990 | 5 | 0 | Imprisonment | Pensioner killers. They killed their victims with objects they brought to the crime scene or found there. They also used punches and stomping on lying victims. The two attacked did not die until several days after the attack. Initially, all three perpetrators received a life sentence, Gaži and Daniš's sentence was reduced to 25 years. | [{{cite web |last1=Mayer |first1=Vladimír |title=Krimiseriál: Vraždy pro pár korun! |url=https://chrudimsky.denik.cz/cerna_kronika/krimi_koruny20070913.html |website=Chrudimský deník |access-date=2 August 2023 |language=cs |date=13 September 2007}}] |
| Martin Lecián | Moravian horror | 1927 | 4 | 0 | Executed | Murdered three policemen and a prison officer during escape attempt. He also attempted to kill seven other policemen. | |
| Jaroslava Fabiánová | The Czech Aileen Wuornos | 1981–2003 | 4 | 0 | Life imprisonment | | |
| Jan Philopon Dambrovský | | 1572–1585 | 4 | 0 | Executed | Roman Catholic priest and dean of the Olomouc chapter, responsible for the poisoning of four archbishops. | |
| Svatoslav Štěpánek | The Roudnice Monster | 1926–1936 | 3 | 2+ | Executed | Killed at least one child and two women around Roudnice nad Labem between 1926 and 1936. Convicted of these crimes and two attempted murders, he was sentenced to death and subsequently executed in 1938. | |
| Jan Janeček | Serynek | 1870 | 3 | 1+ | Executed | Robbed and murdered several people. Executed in 1871; last person to be publicly executed in Austria-Hungary. | [{{cite web |title=Jan Janeček |url=https://www.serialkillers.cz/jan-janecek |website=SerialKillers.cz |access-date=10 August 2023 |language=cs}}] |
| Jaroslav Malý | | 1986 | 3 | 1 | Life imprisonment | After his release from prison, where he spent 30 months for two crimes of a violent nature, he decided to take revenge on society with one murder for every ten months of his "unjust" sentence and killed three random victims with a shot to the head. He was sentenced to death for his act, which was later commuted to life imprisonment. In prison, he likely helped a fellow inmate commit suicide. | [{{cite web |title=Nejkrutější vrah v Česku: Nedá se nic dělat, děvenko, řekl a střelil matku novorozence do hlavy |url=https://www.blesk.cz/clanek/zpravy-krimi-zapomenute-zlociny/590687/nejkrutejsi-vrah-v-cesku-neda-se-nic-delat-devenko-rekl-a-strelil-matku-novorozence-do-hlavy.html |website=Blesk.cz |access-date=2 August 2023 |language=cs |date=24 October 2020}}] |
| Karel Dvořák | | 1959–1973 | 3 | 0 | Executed | | [{{cite web |title=Karel Dvořák |url=https://www.serialkillers.cz/zapomenuty-seriovy-vrah |website=SerialKillers.cz |access-date=10 August 2023 |language=cs}}] |
| Miloslav Sláma | | 2001 | 3 | 0 | Life imprisonment | Brutally attacked and robbed elderly women. Four victims survived his attacks. | [{{cite web |last1=Kačánová |first1=Jitka |title=Vrah důchodkyň Miloslav Sláma stráví zbytek života ve vězení |url=https://nasregion.cz/vrah-duchodkyn-miloslav-slama-stravi-ve-vezeni-zbytek-zivota-234022/|website=nasregion.cz |access-date=2 August 2023}}] |
| Jiří Straka | Spartakiad killer | 1985 | 3 | 0 | Released in 2004 | | |
| David Virgulák | Taxi killer | 2014 | 3 | 0 | Life imprisonment | Killed three taxi drivers. | [{{Cite news |date=January 4, 2018 |title=Nejvyšší soud potvrdil vrahovi tří taxikářů doživotní trest |trans-title=The Supreme Court upheld the life sentence for the murderer of three taxi drivers |url=https://www.novinky.cz/clanek/krimi-nejvyssi-soud-potvrdil-vrahovi-tri-taxikaru-dozivotni-trest-40056521 |archive-url=https://archive.today/20240629184624/https://web.archive.org/web/20230327195915/https://www.novinky.cz/clanek/krimi-nejvyssi-soud-potvrdil-vrahovi-tri-taxikaru-dozivotni-trest-40056521 |archive-date=June 29, 2024 |work=Novinky.cz |language=cs}}] |
| Jaroslav Hejna | | 2003 | 3 | 0 | Life imprisonment | Murdered three people for selfish reasons. His friend Marek Dohnal helped him remove the bodies of the first two victims. | [{{cite web |title=Soud zpřísnil vrahovi trest na doživotí |url=https://www.idnes.cz/zpravy/cerna-kronika/soud-zprisnil-vrahovi-trest-na-dozivoti.A050222_124926_krimi_mad |website=iDNES.cz |access-date=2 August 2023 |language=cs |date=22 February 2005}}] |
| Michal Semanský | | 2009–2012 | 3 | 0 | Life imprisonment | Killed during robberies in senior citizens' homes. Two of the victims were his relatives. | [{{Cite news |last=Mikuláš Klang |date=January 15, 2013 |title=Je to dobrý člověk, hájil právník vraha tří lidí. Soud potvrdil doživotí |trans-title=He is a good person, the lawyer defended the killer of three people. The court confirmed the sentence |url=https://www.idnes.cz/zpravy/cerna-kronika/dozivoti-pro-vraha-semanskeho.A130115_121523_krimi_klm |archive-url=https://archive.today/20240627201439/https://www.idnes.cz/zpravy/cerna-kronika/dozivoti-pro-vraha-semanskeho.A130115_121523_krimi_klm |archive-date=June 27, 2024 |work=iDnes |language=cs}}] |
| Roman Horáček | | 1990–1991 | 3 | 0 | Murdered in 2006 | Murdered a doctor, his ex-wife and her sister in Litoměřice | [{{Cite news |last=Elmira Talířová |date=April 8, 2022 |title=Vraha našli mrtvého v římovské přehradě. Několik let starý případ policie stále neuzavřela |trans-title=The murderer was found dead in the Římov Reservoir. The police still haven't closed the case, which is several years old |url=https://budejcka.drbna.cz/krimi/34486-vraha-nasli-mrtveho-v-rimovske-prehrade-nekolik-let-stary-pripad-policie-stale-neuzavrela.html |archive-url=https://archive.today/20240627200915/https://budejcka.drbna.cz/krimi/34486-vraha-nasli-mrtveho-v-rimovske-prehrade-nekolik-let-stary-pripad-policie-stale-neuzavrela.html |archive-date=June 27, 2024 |work=Budějcká Drbna |language=cs}}] |
| Karel Šťovíček | | 1998–2020 | 3 | 0 | Life imprisonment | Caused the death of a pensioner during a robbery; after release, murdered two women in sexual encounters | [{{Cite news |last=Petr Roubíček |date=April 12, 2021 |title=Za dvojnásobnou vraždu doživotí, potvrdil Krajský soud v Hradci Králové |trans-title=Life imprisonment for double murder, confirmed by the Regional Court in Hradec Králové |url=https://hradecka.drbna.cz/zpravy/9533-za-dvojnasobnou-vrazdu-dozivoti-potvrdil-krajsky-soud-v-hradci-kralove.html |archive-url=https://archive.today/20240627201936/https://hradecka.drbna.cz/zpravy/9533-za-dvojnasobnou-vrazdu-dozivoti-potvrdil-krajsky-soud-v-hradci-kralove.html |archive-date=June 27, 2024 |work=Hradecká drbna |language=cs}}] |