| class="sortable" border="1" cellpadding="5" cellspacing="0" style="border:1px solid #aaa; width:1185px; border-collapse:collapse"
| align="left" style="background:#F2F3F4" width="87px" | Enzyme
| align="center" style="background:#F2F3F4" width="88px" | PDB code
| align="left" style="background:#F2F3F4" width="290px" | Source
| align="left" style="background:#F2F3F4" width="180px" | Recognition sequence
| align="left" style="background:#F2F3F4" width="320px" | Cut
| align="left" style="background:#F2F3F4" width="215px" | Isoschizomers |
| Bac36I
|
| Bacillus alcalophilus 36
| 5' GGNCC
3' CCNGG
| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'
| AspS9I, AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV |
BaeI[{{Cite journal|vauthors=Sears LE, Zhou B, Aliotta JM, Morgan RD, Kong H | title = BaeI, another unusual BcgI-like restriction endonuclease | journal = Nucleic Acids Res| volume = 24 | issue = 18| pages = 3590–2|date=September 1996 | pmid = 8836187| pmc = 146138 | doi = 10.1093/nar/24.18.3590}}]
|
| Bacillus sphaericus
| 5' ACN4GTAYC
3' TGN4CATYG
| 5' ---ACN4GTAYCN6NNNNNN {{pad|2em}} --- 3'
3' ---TGN4CATYGN6N {{pad|2em}} NNNNN--- 5'
| {{pad}}— None in May 2010 — |
BalI[{{Cite journal|vauthors=Ueno H, Kato I, Ishino Y | title =Cloning and expression of the BalI restriction-modification system | journal = Nucleic Acids Res| volume = 24 | issue = 12| pages = 2268–70|date=June 1996 | pmid = 8710495| pmc = 145948 | doi = 10.1093/nar/24.12.2268}}][{{Cite journal|vauthors=Gelinas RE, Myers PA, Weiss GH, Roberts RJ, Murray K | title = A specific endonuclease from Brevibacterium albidum| journal = J Mol Biol | volume = 114 | issue = 3 | pages = 433–40 |date=August 1977 | pmid = 909093 | doi = 10.1016/0022-2836(77)90260-1}}]
|
| Brevibacterium albidum
| 5' TGGCCA
3' ACCGGT
| 5' ---TGG {{pad|2em}} CCA--- 3'
3' ---ACC {{pad|2em}} GGT--- 5'
| |
| Bal228I
|
| Bacillus alcalophilus 228
| 5' GGNCC
3' CCNGG
| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'
| AspS9I, AvcI, BavAII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV |
BamHI[{{Cite journal|vauthors=Wei H, Therrien C, Blanchard A, Guan S, Zhu Z | title =The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases | journal =Nucleic Acids Res | volume = 36| issue = 9| pages = e50|date=May 2008 | pmid = 18413342| pmc =2396408 | doi =10.1093/nar/gkn182 }}][{{Cite journal|vauthors=Nishigaki K, Kaneko Y, Wakuda H, Husimi Y, Tanaka T | title = Type II restriction endonucleases cleave single-stranded DNAs in general | journal = Nucleic Acids Res | volume = 13 | issue = 16 | pages = 5747–60 |date=August 1985 | pmid = 2994012| pmc = 321909 | doi = 10.1093/nar/13.16.5747}}][{{Cite journal|vauthors=Nelson M, Christ C, Schildkraut I | title = Alteration of apparent restriction endonuclease recognition specificities by DNA methylases | journal = Nucleic Acids Res | volume = 12 | issue = 13 | pages = 5165–73 |date=July 1984 | pmid = 6087274| pmc = 318911 | doi = 10.1093/nar/12.13.5165}}][{{Cite journal|vauthors=Nelson PS, Papas TS, Schweinfest CW | title = Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios | journal = Nucleic Acids Res | volume = 21 | issue = 3 | pages = 681–6 |date=February 1993 | pmid = 8441677| pmc = 309169 | doi = 10.1093/nar/21.3.681}}][{{Cite journal|vauthors=Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW | title = A general method for the purification of restriction enzymes| journal = Nucleic Acids Res | volume = 5 | issue = 7| pages = 2373–80|date=July 1978| pmid = 673857| pmc = 342170 | doi = 10.1093/nar/5.7.2373 }}][{{Cite journal|vauthors=Huang LH, Farnet CM, Ehrlich KC, Ehrlich M | title = Digestion of highly modified bacteriophage DNA by restriction endonucleases | journal = Nucleic Acids Res | volume = 10 | issue = 5 | pages = 1579–91 |date=March 1982 | pmid = 6280151| pmc = 320551 | doi = 10.1093/nar/10.5.1579}}][{{Cite journal|vauthors=Wilson GA, Young FE | title =Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H | journal = J Mol Biol| volume = 97| issue = 1| pages = 123–5|date=September 1975| pmid = 1177312 | doi = 10.1016/S0022-2836(75)80028-3}}][{{Cite journal|vauthors=Roberts RJ, Wilson GA, Young FE | title = Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H | journal = Nature | volume = 265| issue = 5589| pages =82–4 |date=January 1977 | pmid = 834250 | doi = 10.1038/265082a0| bibcode = 1977Natur.265...82R | s2cid = 4282096 }}][{{Cite journal|vauthors=Brooks JE, Benner JS, Heiter DF, Silber KR, Sznyter LA, Jager-Quinton T, Moran LS, Slatko BE, Wilson GG, Nwankwo DO | title =Cloning the BamHI restriction modification system | journal = Nucleic Acids Res | volume = 17 | issue = 3| pages = 979–97|date=February 1989 | pmid = 2537955| pmc = 331717 | doi =10.1093/nar/17.3.979 }}][{{Cite journal|vauthors=Viadiu H, Aggarwal AK | title = Structure of BamHI bound to nonspecific DNA: a model for DNA sliding | journal = Mol. Cell | volume = 5 | issue = 5 | pages = 889–95 |date=May 2000 | pmid = 10882125 | doi = 10.1016/S1097-2765(00)80329-9| doi-access = free }}][{{Cite journal|vauthors=George J, Chirikjian JG | title = Sequence-specific endonuclease BamHI: relaxation of sequence recognition| journal =Proc Natl Acad Sci USA | volume = 79| issue = 8| pages = 2432–6|date=April 1982 | pmid = 6283522| pmc = 346212 | doi =10.1073/pnas.79.8.2432 | bibcode = 1982PNAS...79.2432G| doi-access = free}}]
[{{Cite journal|vauthors=Mukhopadhyay P, Roy KB | title = Sequence-specific BamHI methylase. Purification and characterization | journal = The Journal of Biological Chemistry| volume = 259| issue = 16| pages = 10357–62| year = 1984| doi = 10.1016/S0021-9258(18)90972-5 | pmid = 6469968 | doi-access = free }}][{{Cite journal|vauthors=Robinson CR, Sligar SG | title = Heterogeneity in molecular recognition by restriction endonucleases: osmotic and hydrostatic pressure effects on BamHI, Pvu II, and EcoRV specificity | journal = Proc Natl Acad Sci USA | volume = 92 | issue = 8 | pages = 3444–8 |date=April 1995 | pmid = 7724581| pmc = 42183 | doi = 10.1073/pnas.92.8.3444| bibcode = 1995PNAS...92.3444R | doi-access = free }}][{{Cite journal|vauthors=Viadiu H, Aggarwal AK | title = The role of metals in catalysis by the restriction endonuclease BamHI | journal = Nat Struct Biol | volume = 5 | issue = 10 | pages = 910–6 |date=October 1998 | pmid = 9783752 | doi = 10.1038/2352| s2cid = 25062657 }}][{{Cite journal|vauthors=Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK | title = Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding | journal = Science | volume = 269 | issue = 5224 | pages = 656–63 |date=August 1995 | pmid = 7624794 | doi = 10.1126/science.7624794| bibcode = 1995Sci...269..656N }}][{{Cite journal|vauthors=Roy KB, Vrushank D | title = Bam HI cleaves the self complementary dodecamer d-CGCGGAGCCGCG, before the two G's and possibly binds in the DNA major groove | journal = Biochem Mol Biol Int | volume = 36 | issue = 4 | pages = 759–70 |date=July 1995 | pmid = 8528138 }}][{{Cite journal|vauthors=Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK | title = Structure of restriction endonuclease bamhi phased at 1.95 A resolution by MAD analysis | journal = Structure | volume = 2 | issue = 5 | pages = 439–52 |date=May 1994 | pmid = 8081758 | doi = 10.1016/S0969-2126(00)00045-9| doi-access = free }}][{{Cite journal|vauthors=Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK | title = Structure of restriction endonuclease BamHI and its relationship to EcoRI | journal = Nature | volume = 368 | issue = 6472 | pages = 660–4 |date=April 1994 | pmid = 8145855 | doi = 10.1038/368660a0| bibcode = 1994Natur.368..660N | s2cid = 2999008 }}][{{Cite journal|vauthors=Roy KB, Vrushank D, Jayaram B | title = Use of isotope-dilution phenomenon to advantage in the determination of kinetic constants Km and Kcat for BamHI restriction endonuclease: an empirical and iterative approach | journal = Anal Biochem | volume = 220 | issue = 1 | pages = 160–4 |date=July 1994 | pmid = 7978240 | doi = 10.1006/abio.1994.1313 }}][{{Cite journal|vauthors=Brooks JE, Nathan PD, Landry D, Sznyter LA, Waite-Rees P, Ives CL, Moran LS, Slatko BE, Benner JS | title = Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts | journal = Nucleic Acids Res | volume = 19 | issue = 4 | pages = 841–50 |date=February 1991 | pmid = 1901989| pmc = 333720 | doi = 10.1093/nar/19.4.841}}][{{Cite journal| doi = 10.1093/protein/11.10.931|vauthors=Mukhopadhyay P, Roy KB | title = Protein engineering of BamHI restriction endonuclease: replacement of Cys54 by Ala enhances catalytic activity | journal = Protein Eng | volume = 11 | issue = 10 | pages = 931–5 |date=October 1998 | pmid = 9862213 | doi-access = free }}]
| align="center" | [http://www.pdb.org/pdb/explore/explore.do?structureId=1BAM 1BAM]
| Bacillus amyloliquefaciens H
| 5' GGATCC
3' CCTAGG
| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'
| AccEBI, AliI, ApaCI, AsiI, Bce751I, Bsp98I, Bsp4009I, BspAAIII, CelI, Nsp29132II, NspSAIV, SolI, SurI |
| BamNII
|
| Bacillus amyloliquefaciens N
| 5' GGWCC
3' CCWGG
| 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5'
| BcuAI, BsrAI, CauI, EagMI, FdiI, HgiCII, HgiJI, SinI |
BanI[{{Cite journal|vauthors=Sugisaki H, Maekawa Y, Kanazawa S, Takanami M | title = New restriction endonucleases from Acetobacter aceti and Bacillus aneurinolyticus | journal = Nucleic Acids Res | volume = 10 | issue = 19 | pages = 5747–52 |date=October 1982 | pmid = 6292849| pmc = 320926 | doi = 10.1093/nar/10.19.5747 }}][{{Cite journal|vauthors=Schildkraut I, Lynch J, Morgan R | title = The cleavage site for the restriction endonucleases BanI and HgiC I is 5' ...G decreases GPyPuCC ...3' | journal = Schildkraut I, Lynch J, Morgan R | volume = 15 | issue = 13 | pages = 5492 |date=July 1987 | pmid = 3037499| pmc = 305987 | doi = 10.1093/nar/15.13.5492 }}]
|
| Bacillus aneurinolyticus
| 5' GGYRCC
3' CCRYGG
| 5' ---G {{pad|2em}} GYRCC--- 3'
3' ---CCRYG {{pad|2em}} G--- 5'
| AccB1I, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI |
BanII|
| Bacillus aneurinolyticus
| 5' GRGCYC
3' CYCGRG
| 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5'
| |
BanIII|
| Bacillus aneurinolyticus
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| AagI, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI |
| BanAI
|
| Bacillus anthracis
| 5' GGCC
3' CCGG
| 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5'
| |
| BasI
|
| Bacillus sp.
| 5' CCAN5TGG
3' GGTN5ACC
| 5' ---CCANNNN {{pad|2em}} NTGG--- 3'
3' ---GGTN {{pad|2em}} NNNNACC--- 5'
| AccB7I, AcpII, Asp10HII, Esp1396I, PflBI, PflMI, Van91I |
| BauI
|
| Bacillus aquaemaris RFL1
| 5' CACGAG
3' GTGCTC
| 5' ---C {{pad|2em}} ACGAG--- 3'
3' ---GTGCT {{pad|2em}} C--- 5'
| |
| BavI
|
| Bacillus alvei
| 5' CAGCTG
3' GTCGAC
| 5' ---CAG {{pad|2em}} CTG--- 3'
3' ---GTC {{pad|2em}} GAC--- 5'
| |
| BavAI
|
| Bacillus alvei A
| 5' CAGCTG
3' GTCGAC
| 5' ---CAG {{pad|2em}} CTG--- 3'
3' ---GTC {{pad|2em}} GAC--- 5'
| |
| BavAII
|
| Bacillus alvei A
| 5' GGNCC
3' CCNGG
| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'
| AspS9I, AvcI, BavBII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV |
| BavBI
|
| Bacillus alvei B
| 5' CAGCTG
3' GTCGAC
| 5' ---CAG {{pad|2em}} CTG--- 3'
3' ---GTC {{pad|2em}} GAC--- 5'
| |
| BavBII
|
| Bacillus alvei B
| 5' GGNCC
3' CCNGG
| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'
| AspS9I, Bac36I, BavAII, BshKI, BspBII, Bsu54I, FmuI, Pde12I |
| BavCI
|
| Bacillus alvei C
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| AagI, BanIII, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI |
BbeI[{{Cite journal|vauthors=Khosaka T, Sakurai T, Takahashi H, Saito H | title = A new site-specific endonuclease Bbei from Bifidobacterium breve | journal = Gene | volume = 17 | issue = 2 | pages = 117–22 |date=February 1982 | pmid = 6282709 | doi = 10.1016/0378-1119(82)90063-4}}]
|
| Bifidobacterium breve YIT4006
| 5' GGCGCC
3' CCGCGG
| 5' ---GGCGC {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} CGCGG--- 5'
| |
| BbiII
|
| Bifidobacterium bifidum YIT4007
| 5' GRCGYC
3' CYGCRG
| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'
| AcyI, AhaII, BbiII, HgiHII, Hin1I, Hsp92I, Msp17I, PamII |
| Bbi24I
|
| Bifidobacterium bifidum S-24
| 5' ACGCGT
3' TGCGCA
| 5' ---A {{pad|2em}} CGCGT--- 3'
3' ---TGCGC {{pad|2em}} A--- 5'
| |
| BbrI
|
| Bordetella bronchiseptica 4994
| 5' AAGCTT
3' TTCGAA
| 5' ---A {{pad|2em}} AGCTT--- 3'
3' ---TTCGA {{pad|2em}} A--- 5'
| |
| Bbr7I
|
| Bacillus brevis 7
| 5' GAAGAC
3' CTTCTG
| 5' ---GAAGACN6N {{pad|2em}} NNNNN--- 3'
3' ---CTTCTGN6NNNNNN {{pad|2em}} --- 5'
| |
BbrPI[{{Cite journal|vauthors=Vesely Z, Schmitz GG, Jarsch M, Kessler C | title = BbrPI, a novel PmacI isoschizomer from Bacillus brevis recognizing 5'-CAC/GTG-3' | journal = Nucleic Acids Res | volume = 18 | issue = 11 | pages = 3423 |date=June 1990 | pmid = 2162525| pmc = 330975 | doi = 10.1093/nar/18.11.3423}}][{{Cite journal|vauthors=Kafri T, Hershko A, Razin A | title = Probing CpG methylation at CACGTG with BbrPI restriction enzyme | journal = Nucleic Acids Res | volume = 21 | issue = 12 | pages = 2950 |date=June 1993 | pmid = 8332512| pmc = 309701 | doi = 10.1093/nar/21.12.2950}}]
|
| Bacillus brevis
| 5' CACGTG
3' GTGCAC
| 5' ---CAC {{pad|2em}} GTG--- 3'
3' ---GTG {{pad|2em}} CAC--- 5'
| AcvI, BcoAI, Eco72I, PmaCI, PmlI, PspCI |
BbsI[{{Cite journal|vauthors=Protozanova E, Demidov VV, Nielsen PE, Frank-Kamenetskii MD | title = Pseudocomplementary PNAs as selective modifiers of protein activity on duplex DNA: the case of type IIs restriction enzymes | journal = Nucleic Acids Res | volume = 31 | issue = 14 | pages = 3929–35 |date=July 2003 | pmid = 12853608| pmc = 165965 | doi = 10.1093/nar/gkg450}}]
|
| Bacillus laterosporus
| 5' GAAGAC
3' CTTCTG
| 5' ---GAAGACNN {{pad|2em}} NNNN--- 3'
3' ---CTTCTGNNNNNN {{pad|2em}} --- 5'
| |
BbuI[{{Cite journal|vauthors=Aubert E, Spurgeon S, Ray W, Davies J | title = Purification and characterization of an isoschizomer of SphI from Bacillus circulans | journal = Nucleic Acids Res | volume = 18 | issue = 20 | pages = 6152 |date=October 1990 | pmid = 2146591| pmc = 332440 | doi = 10.1093/nar/18.20.6152}}]
|
| Bacillus circulans
| 5' GCATGC
3' CGTACG
| 5' ---GCATG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GTACG--- 5'
| |
BbvI[{{Cite journal|vauthors=Gingeras TR, MIlazzo JP, Roberts RJ | title = A computer assisted method for the determination of restriction enzyme recognifion sites | journal = Nucleic Acids Res | volume = 5 | issue = 11 | pages = 4105–27 |date=November 1978 | pmid = 724510| pmc = 342737 | doi = 10.1093/nar/5.11.4105}}][{{Cite journal|vauthors=Hattman S, Keister T, Gottehrer A | title = Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis | journal = J Mol Biol | volume = 124 | issue = 4 | pages = 701–11 |date=October 1978 | pmid = 712853 | doi = 10.1016/0022-2836(78)90178-X}}]
|
| Bacillus brevis
| 5' GCAGC
3' CGTCG
| 5' ---GCAGCN7N {{pad|2em}} NNNN--- 3'
3' ---CGTCGN7NNNNN {{pad|2em}} --- 5'
| AlwXI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I |
BbvII[{{Cite journal| doi = 10.1016/0014-5793(84)80973-4|vauthors=Matvienko NI, Pachkunov DM, Kramarov VM | title = The recognition sequence of site-specific endonuclease BbvII from Bacillus brevis 80 | journal = FEBS Lett | volume = 177 | pages = 23–36 | year = 1984| doi-access = free }}]
|
| Bacillus brevis 80
| 5' GAAGAC
3' CTTCTG
| 5' ---GAAGACNN {{pad|2em}} NNNN--- 3'
3' ---CTTCTGNNNNNN {{pad|2em}} --- 5'
| |
Bbv12I[{{Cite journal|vauthors=Repin VE, Rechkunova NI, Degtyarev SK, Hachaturyan AA, Afrikyan EK | title = Aerobic spore-forming bacteria as producers of restriction endonucleases | journal = Biol J Armenia | volume = 42 | pages = 969–72 | year = 1989}}]
|
| Bacillus brevis 12
| 5' GWGCWC
3' CWCGWG
| 5' ---GWGCW {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} WCGWG--- 5'
|
{|
| Alw21I, AspHI, Bsh45I, BsiHKAI, |
| HgiAI,
| style="background: darkgray; color: white" | {{pad|0.1em}}HpyF46II,{{pad|0.1em}}
| MspV281I |
|-
|Bbv16II
|
| Bacillus brevis 16
| 5' GAAGAC
3' CTTCTG
| 5' ---GAAGACNN {{pad|2em}} NNNN--- 3'
3' ---CTTCTGNNNNNN {{pad|2em}} --- 5'
|
|-
|BbvAI
|
| Bacillus brevis A
| 5' GAAN4TTC
3' CTTN4AAG
| 5' ---GAANN {{pad|2em}} NNTTC--- 3'
3' ---CTTNN {{pad|2em}} NNAAG--- 5'
| Asp700I, MroXI, PdmI, XmnI
|-
|BbvAII
|
| Bacillus brevis A
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| BavCI, Bci29I, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
|-
|BbvAIII
|
| Bacillus brevis A
| 5' TCCGGA
3' AGGCCT
| 5' ---T {{pad|2em}} CCGGA--- 3'
3' ---AGGCC {{pad|2em}} T--- 5'
| AccIII, Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Bsu23I, Kpn2I
|-
|BbvBI
|
| Bacillus brevis B
| 5' GGYRCC
3' CCRYGG
| 5' ---G {{pad|2em}} GYRCC--- 3'
3' ---CCRYG {{pad|2em}} G--- 5'
| BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
|-
|BbvCI[{{Cite journal|vauthors=Heiter DF, Lunnen KD, Wilson GG | title = Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI | journal = J Mol Biol | volume = 348 | issue = 3 | pages = 631–40 |date=May 2005 | pmid = 15826660 | doi = 10.1016/j.jmb.2005.02.034}}]
|
| Bacillus brevis
| 5' CCTCAGC
3' GGAGTCG
| 5' ---CC {{pad|2em}} TCAGC--- 3'
3' ---GGAGT {{pad|2em}} CG--- 5'
| AbeI
|-
|Bca77I
|
| Bacillus caldolyticus
| 5' WCCGGW
3' WGGCCW
| 5' ---W {{pad|2em}} CCGGW--- 3'
3' ---WGGCC {{pad|2em}} W--- 5'
|
|-
|BccI
|
| Bacteroides caccae
| 5' CCATC
3' GGTAG
| 5' ---CCATCNNNN {{pad|2em}} N--- 3'
3' ---GGTAGNNNNN {{pad|2em}} --- 5'
|
|-
|Bce4I
|
| Bacillus cereus B4
| 5' GCN7GC
3' CGN7CG
| 5' ---GCNNNNN {{pad|2em}} NNGC--- 3'
3' ---CGNN {{pad|2em}} NNNNNCG--- 5'
|
|-
|Bce22I
|
| Bacillus cereus 22
| 5' GGNCC
3' CCNGG
| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'
| AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, FmuI, Pde12I
|-
|Bce83I[{{Cite journal|author1=Matvienko NN |author2=Kramarov VM |author3=Ivanov LYu |author4=Matvienko NI | title = Bce83I, a restriction endonuclease from Bacillus cereus 83 which recognizes novel nonpalindromic sequence 5'-CTTGAG-3' and is stimulated by S-adenosylmethionine | journal = Nucleic Acids Res | volume = 20 | issue = 7 | pages = 1803 |date=April 1992 | pmid = 1315959| pmc = 312275 | doi = 10.1093/nar/20.7.1803 }}]
|
| Bacillus cereus 83
| 5' CTTGAG
3' GAACTC
| 5' ---CTTGAGN13NNN {{pad|2em}} --- 3'
3' ---GAACTCN13N {{pad|2em}} NN--- 5'
|
|-
|Bce243I
|
| Bacillus cereus
| 5' GATC
3' CTAG
| 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5'
| Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
|-
|Bce751I
|
| Bacillus cereus 751
| 5' GGATCC
3' CCTAGG
| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'
| BamHI, Bce751I, BnaI, Bsp98I, Bsp4009I, BstI, NspSAIV, Pfl8I
|-
|BceAI
|
| Bacillus cereus 1315
| 5' ACGGC
3' TGCCG
| 5' ---ACGGCN11N {{pad|2em}} NN--- 3'
3' ---TGCCGN11NNN {{pad|2em}} --- 5'
|
|-
|BceBI
|
| Bacillus cereus 1323
| 5' CGCG
3' GCGC
| 5' ---CG {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} GC--- 5'
| AccII, Bsh1236I, BtkI, Csp68KVI, FalII, FauBII, FnuDII, SelI, ThaI
|-
|BceCI
|
| Bacillus cereus 1195
| 5' GCN7GC
3' CGN7CG
| 5' ---GCNNNNN {{pad|2em}} NNGC--- 3'
3' ---CGNN {{pad|2em}} NNNNNCG--- 5'
|
|-
|BcefI[{{Cite journal|vauthors=Venetianer P, Orosz A | title = BcefI, a new type IIS restriction endonuclease | journal = Nucleic Acids Res | volume = 16 | issue = 7 | pages = 3053–60 |date=April 1988 | pmid = 2835751| pmc = 336451 | doi = 10.1093/nar/16.7.3053 }}]
|
| Bacillus cereus fluorescens
| 5' ACGGC
3' TGCCG
| 5' ---ACGGCN10NN {{pad|2em}} N--- 3'
3' ---TGCCGN10NNN {{pad|2em}} --- 5'
|
|-
|BcgI[{{Cite journal|vauthors=Kong H, Roemer SE, Waite-Rees PA, Benner JS, Wilson GG, Nwankwo DO | title = Characterization of BcgI, a new kind of restriction-modification system | journal = J Biol Chem | volume = 269 | issue = 1 | pages = 683–90 |date=January 1994 | doi = 10.1016/S0021-9258(17)42403-3 | pmid = 8276869 | doi-access = free }}][{{Cite journal|vauthors=Kong H, Morgan RD, Maunus RE, Schildkraut I | title = A unique restriction endonuclease, BcgI, from Bacillus coagulans | journal = Nucleic Acids Res | volume = 21 | issue = 4 | pages = 987–91 |date=February 1993 | pmid = 8451198| pmc = 309233 | doi = 10.1093/nar/21.4.987}}]
|
| Bacillus coagulans
| 5' CGAN6TGC
3' GCTN6ACG
| 5' ---CGAN6TGCN9NNN {{pad|2em}} --- 3'
3' ---GCTN6ACGN9N {{pad|2em}} NN--- 5'
|
|-
|Bci29I
|
| Bacillus circulans 29
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| BavCI, BciBI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
|-
|BciBI
|
| Bacillus circulans B
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| BavCI, BcmI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I
|-
|BciBII
|
| Bacillus circulans B
| 5' CCWGG
3' GGWCC
| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'
| AjnI, ApyI, BptI, Bst1I, BstOI, BstM6I, Bst2UI, EcoRII, MvaI
|-
|BciVI[{{Cite journal|vauthors=Roberts RJ, Macelis D | title = REBASE-restriction enzymes and methylases | journal = Nucleic Acids Res | volume = 25 | issue = 1 | pages = 248–62 |date=January 1997 | pmid = 9016548| pmc = 146408 | doi = 10.1093/nar/25.1.248 }}]
|
| Bacillus circulans
| 5' GTATCC
3' CATAGG
| 5' ---GTATCCN4NN {{pad|2em}} --- 3'
3' ---CATAGGN4N {{pad|2em}} N--- 5'
| BfuI
|-
|BclI[{{Cite journal|vauthors=Rowland GC, Lim PP, Glass RE | title = 'Stop-codon-specific' restriction endonucleases: their use in mapping and gene manipulation | journal = Gene | volume = 116 | issue = 1 | pages = 21–6 |date=July 1992 | pmid = 1628840 | doi = 10.1016/0378-1119(92)90624-X}}][{{Cite journal|vauthors=Bingham AH, Atkinson T, Sciaky D, Roberts RJ | title = A specific endonuclease from Bacillus caldolyticus | journal = Nucleic Acids Res | volume = 5 | issue = 10 | pages = 3457–67 |date=October 1978 | pmid = 724493| pmc = 342687 | doi = 10.1093/nar/5.10.3457}}]
|
| Bacillus caldolyticus
| 5' TGATCA
3' ACTAGT
| 5' ---T {{pad|2em}} GATCA--- 3'
3' ---ACTAG {{pad|2em}} T--- 5'
|
| AbaI, BsiQI, BspXII, |
| BstT7I, FbaI, Ksp22I,
| style="background: darkgray; color: white" | {{pad|0.1em}}ParI{{pad|0.1em}} |
|-
|BcmI
|
| Bacillus sp.
| 5' ATCGAT
3' TAGCTA
| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'
| BsuTUI, Bsu15I, BavCI, Bli86I, BspZEI, Rme21I, BseCI, BdiI
|-
|BcnI[{{Cite journal|vauthors=Janulaitis A, Klimasauskas S, Petrusyte M, Butkus V | title = Cytosine modification in DNA by BcnI methylase yields N4-methylcytosine | journal = FEBS Lett | volume = 161 | issue = 1 | pages = 131–4 |date=September 1983 | pmid = 6884523 | doi = 10.1016/0014-5793(83)80745-5| doi-access = free }}][{{Cite journal|vauthors=Janulaitis AA, Petrusite MA, Jaskelavicene BP, Krayev AS, Skryabin KG, Bayev AA | title = A new restriction endonuclease BcnI from Bacillus centrosporus RFL 1 | journal = FEBS Lett | volume = 137 | issue = 2 | pages = 178–80 |date=January 1982 | pmid = 6277689 | doi = 10.1016/0014-5793(82)80343-8| doi-access = free }}][{{Cite journal|vauthors=Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M | title = Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA | journal = J Mol Biol | volume = 369 | issue = 3 | pages = 722–34 |date=June 2007 | pmid = 17445830 | doi = 10.1016/j.jmb.2007.03.018}}]
| align="center" | [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2ODH 2ODH]
| Bacillus centrosporus RFL1
| 5' CCSGG
3' GGSCC
| 5' ---CC {{pad|2em}} SGG--- 3'
3' ---GGS {{pad|2em}} CC--- 5'
|
| AhaI, AseII, AsuC2I,
| style="background: darkgray; color: white" | BpuMI,
| CauII, |
| colspan="3"| Eco1831I, HgiS22I,Kpn49kII, NciI |
|-
|BcoI[{{Cite journal| doi = 10.1111/j.1574-6968.1990.tb03988.x|vauthors=Leung SM, Kam KM, Chan KY, Shaw PC | title = Purification and characterization of restriction endonuclease BcoI from a soil isolate of Bacillus coagulans | journal = FEMS Microbiol Lett | volume = 66| issue = 2 | pages = 153–6 | year = 1990| pmid = 1936944| doi-access = free }}]
|
| Bacillus coagulans SM 1
| 5' CYCGRG
3' GRGCYC
| 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5'
| Ama87I, AquI, BsoBI, BstSI, Eco88I, NspSAI, OfoI, PunAI
|-
|Bco5I
|
| Bacillus coagulans 5
| 5' CTCTTC
3' GAGAAG
| 5' ---CTCTTCN {{pad|2em}} NNN--- 3'
3' ---GAGAAGNNNN {{pad|2em}} --- 5'
|
|-
|Bco27I
|
| Bacillus coagulans 27
| 5' CCGG
3' GGCC
| 5' ---C {{pad|2em}} CGG--- 3'
3' ---GGC {{pad|2em}} C--- 5'
|
|-
|Bco116I
|
| Bacillus coagulans 116
| 5' CTCTTC
3' GAGAAG
| 5' ---CTCTTCN {{pad|2em}} NNN--- 3'
3' ---GAGAAGNNNN {{pad|2em}} --- 5'
|
|-
|Bco118I
|
| Bacillus coagulans 118
| 5' RCCGGY
3' YGGCCR
| 5' ---R {{pad|2em}} CCGGY--- 3'
3' ---YGGCC {{pad|2em}} R--- 5'
|
|-
|BcoAI
|
| Bacillus coagulans AUCM B-732
| 5' CACGTG
3' GTGCAC
| 5' ---CAC {{pad|2em}} GTG--- 3'
3' ---GTG {{pad|2em}} CAC--- 5'
| AcvI, BbrPI, Eco72I, PmaCI, PmlI, PspCI
|-
|BcoKI
|
| Bacillus coagulans
| 5' CTCTTC
3' GAGAAG
| 5' ---CTCTTCN {{pad|2em}} NNN--- 3'
3' ---GAGAAGNNNN {{pad|2em}} --- 5'
|
|-
|BcuI
|
| Bacillus coagulans Vs 29-022
| 5' ACTAGT
3' TGATCA
| 5' ---A {{pad|2em}} CTAGT--- 3'
3' ---TGATC {{pad|2em}} A--- 5'
| AhII, AclNI, SpeI
|-
|BcuAI
|
| Bacillus cereus BKM B-814
| 5' GGWCC
3' CCWGG
| 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5'
| BamNxI, BsrAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinI
|}
:{{Incomplete list|date=September 2010}}
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