MAP-Seq
{{Short description|Method for neuronal projection mapping}}
MAP-Seq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed by Anthony M. Zador and his team at Cold Spring Harbor Laboratory and published in Neuron, a Cell Press magazine.{{Cite journal|last=Kebschull|first=Justus M.|last2=Silva|first2=Pedro Garcia da|last3=Reid|first3=Ashlan P.|last4=Peikon|first4=Ian D.|last5=Albeanu|first5=Dinu F.|last6=Zador|first6=Anthony M.|title=High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA|journal=Neuron|volume=91|issue=5|pages=975–987|doi=10.1016/j.neuron.2016.07.036|pmid=27545715|year=2016|pmc=6640135}} It has been cited over 300 times.
Mechanism
The method works by uniquely labeling neurons in a source region by injecting a viral library encoding a diverse collection of RNA sequences ("barcodes"). The barcode mRNA is expressed at high levels and transported into the axon terminals at distal target projection regions. Following this, the cells from source and putative target regions of interest are harvested, and their RNA is extracted and sequenced. By matching the presence of the unique "barcode" in the source and target tissue, one can map the projections of neuron in a one-to-many fashion.{{Cite news|url=http://www.rna-seqblog.com/revolutionary-method-to-map-brains-at-single-neuron-resolution-successfully-demonstrated/|title=Revolutionary method to map brains at single-neuron resolution successfully demonstrated|date=2016-09-21|access-date=2018-03-29|language=en-US}}
See also
References
External links
- {{Cite web|last=Brouillette|first=Monique|date=2018-04-04|title=New Brain Maps With Unmatched Detail May Change Neuroscience|url=https://www.quantamagazine.org/new-brain-maps-with-unmatched-detail-may-change-neuroscience-20180404|website=Quanta Magazine|language=en}}
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