MSO

• Madison Symphony Orchestra, of Madison, Wisconsin, U.S.
• Melbourne Symphony Orchestra, of Melbourne, Victoria, Australia
• Melbourne Ska Orchestra, of Melbourne, Victoria, Australia
• Milwaukee Symphony Orchestra, of Milwaukee, Wisconsin, U.S.
• Monroe Symphony Orchestra, of Monroe, Louisiana, U.S.
• Montreal Symphony Orchestra, of Montreal, Quebec, Canada

• Martha Stewart Living Omnimedia, a media and merchandising company
• McLaren Special Operations of McLaren Automotive
• Mind Sports Organisation, a vehicle for promoting mental-skill games
• Mind Sports Olympiad

• Methionine sulfoximine, a chemical compound
• Multiple system operator, an operator of multiple cable or direct-broadcast satellite TV systems
• Murashige and Skoog medium, a plant growth medium used in laboratories for cellular cultivation
• Mixed-signal oscilloscope
• Monadic second-order logic, in mathematical logic

• Michigan Southern Railroad (1989), reporting markm MSO
• Missoula Montana Airport, in Montana, U.S., IATA code MSO
• Moston railway station, in Manchester, England, station code MSO

• Maritime security operations, combatting sea–based terrorism and other illegal activities
• Manufacturer Statement of Origin, or Manufacturer's Certificate of Origin
• Mountain Skies Observatory, in Wyoming, U.S.
• A US Navy hull classification symbol: Minesweeper, ocean (MSO)
• Mars Science Orbiter, a proposed spacecraft
• Municipal Services Office (Singapore), a government office

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