Mustard Database
{{Short description|Biological database}}
{{Infobox biodatabase|title=Mustard|logo=|description=Contains AR determinants and curated AR genes identified from metagenomics|scope=Antimicrobial resistance genes and AR Determinants|organism=Bacteria|center=|laboratory=|author=|pmid=30478291|released=|standard=|format=|url={{URL|http://mgps.eu/Mustard/}}|download=[http://mgps.eu/Mustard/index.php?id=db Download]|webservice=|sql=|sparql=|webapp=|standalone=|license=|versioning=|frequency=|curation=|bookmark=yes|version=}}
Mustard is a database that tracks Antimicrobial Resistance Determinants (ARDs). The method by which it tracks ARDs is using their own method adapted from Protein Homology Modelling called Pairwise Comparative Modelling (PCM), which increase specificity protein prediction, especially for distantly related protein homologues.{{Cite journal|last1=Ehrlich|first1=S. Dusko|last2=Kennedy|first2=Sean P.|last3=Doré|first3=Joël|last4=Haimet|first4=Florence|last5=Weiszer|first5=Kevin|last6=Levenez|first6=Florence|last7=Letur|first7=Aline|last8=Léjard|first8=Véronique|last9=Léonard|first9=Pierre|date=January 2019|title=Prediction of the intestinal resistome by a three-dimensional structure-based method|journal=Nature Microbiology|volume=4|issue=1|pages=112–123|doi=10.1038/s41564-018-0292-6|pmid=30478291|s2cid=53757858|issn=2058-5276|url=http://pure-oai.bham.ac.uk/ws/files/56105905/Ruppe_et_al_Prediction_of_the_intestinal_resistome_Nature_Microbiology_2018.pdf|doi-access=free}} Using PCM, 6095 ARDs from 20 families in the human gut microbiota. Antibiotic resistance databases used were ResFinder,{{Cite journal|last1=Zankari|first1=E.|last2=Hasman|first2=H.|last3=Cosentino|first3=S.|last4=Vestergaard|first4=M.|last5=Rasmussen|first5=S.|last6=Lund|first6=O.|last7=Aarestrup|first7=F. M.|last8=Larsen|first8=M. V.|date=2012-07-10|title=Identification of acquired antimicrobial resistance genes|journal=Journal of Antimicrobial Chemotherapy|volume=67|issue=11|pages=2640–2644|doi=10.1093/jac/dks261|pmid=22782487|issn=0305-7453|pmc=3468078}} ARG-ANNOT,{{Cite journal|last1=Gupta|first1=Sushim Kumar|last2=Padmanabhan|first2=Babu Roshan|last3=Diene|first3=Seydina M.|last4=Lopez-Rojas|first4=Rafael|last5=Kempf|first5=Marie|last6=Landraud|first6=Luce|last7=Rolain|first7=Jean-Marc|date=2013-10-21|title=ARG-ANNOT, a New Bioinformatic Tool To Discover Antibiotic Resistance Genes in Bacterial Genomes|journal=Antimicrobial Agents and Chemotherapy|volume=58|issue=1|pages=212–220|doi=10.1128/aac.01310-13|pmid=24145532|issn=0066-4804|pmc=3910750}} the now defunct Lahey Clinic,{{cite web|url=http://www.fibim.unisi.it/REDDB/|title=RED-DB|accessdate=July 27, 2019}} Marilyn Roberts website for tetracycline and macrolide resistance genes{{cite web|url=http://faculty.washington.edu/marilynr/|title=Marilyn Roberts website}} and metagenomics.{{Cite journal|last1=Allen|first1=Heather K|last2=Moe|first2=Luke A|last3=Rodbumrer|first3=Jitsupang|last4=Gaarder|first4=Andra|last5=Handelsman|first5=Jo|date=2008-10-09|title=Functional metagenomics reveals diverse β-lactamases in a remote Alaskan soil|journal=The ISME Journal|volume=3|issue=2|pages=243–251|doi=10.1038/ismej.2008.86|pmid=18843302|s2cid=16496336|issn=1751-7362|doi-access=free}}{{Cite journal|last1=Moore|first1=Aimée M.|last2=Patel|first2=Sanket|last3=Forsberg|first3=Kevin J.|last4=Wang|first4=Bin|last5=Bentley|first5=Gayle|last6=Razia|first6=Yasmin|last7=Qin|first7=Xuan|last8=Tarr|first8=Phillip I.|last9=Dantas|first9=Gautam|date=2013-11-13|title=Pediatric Fecal Microbiota Harbor Diverse and Novel Antibiotic Resistance Genes|journal=PLOS ONE|volume=8|issue=11|pages=e78822|doi=10.1371/journal.pone.0078822|pmid=24236055|pmc=3827270 |bibcode=2013PLoSO...878822M |issn=1932-6203|doi-access=free}}{{Cite journal|last1=Sommer|first1=M. O. A.|last2=Dantas|first2=G.|last3=Church|first3=G. M.|date=2009-08-27|title=Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora|journal=Science|volume=325|issue=5944|pages=1128–1131|doi=10.1126/science.1176950|pmid=19713526|issn=0036-8075|pmc=4720503|bibcode=2009Sci...325.1128S }}