Optimal cutting temperature compound
Image:Tissue for frozen section in cryostat.JPG: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production]]
Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.
Components
From MSDS:{{Cite web
| publisher= Sakura
|url= https://www.sakuraus.com/SakuraWebsite/media/SDS/0000582-03-Rev-F-Tissue-Tek-OCT-Compound-SDS_US-English-(effective-1-29-16)-(1).pdf?ext=.pdf
|title= Safety Data Sheet
|access-date=26 November 2024}}
- 5–15% polyvinyl alcohol
- 1–10% polyethylene glycol
- 75–95% non-reactive ingredients
See also
References
{{reflist}}
Further reading
- {{cite journal |last1=Turbett |first1=Gavin |last2=Sellner |first2=Loryn |title=The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction |journal=Diagnostic Molecular Pathology |volume=6 |issue=5 |pages=298–303 |year=1997 |pmid=9458390 |doi=10.1097/00019606-199710000-00009}}
{{pathology-stub}}