Optimal cutting temperature compound

Image:Tissue for frozen section in cryostat.JPG: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production]]

Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.

Components

From MSDS:{{Cite web

| publisher= Sakura

|url= https://www.sakuraus.com/SakuraWebsite/media/SDS/0000582-03-Rev-F-Tissue-Tek-OCT-Compound-SDS_US-English-(effective-1-29-16)-(1).pdf?ext=.pdf

|title= Safety Data Sheet

|access-date=26 November 2024}}

  • 5–15% polyvinyl alcohol
  • 1–10% polyethylene glycol
  • 75–95% non-reactive ingredients

See also

References

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Further reading

  • {{cite journal |last1=Turbett |first1=Gavin |last2=Sellner |first2=Loryn |title=The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction |journal=Diagnostic Molecular Pathology |volume=6 |issue=5 |pages=298–303 |year=1997 |pmid=9458390 |doi=10.1097/00019606-199710000-00009}}

Category:Histology

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