PEST sequence

{{Short description|Peptide sequence in short-lived proteins}}

A PEST sequence is a peptide sequence that is rich in proline (P), glutamic acid (E), serine (S) and threonine (T). It is associated with proteins that have a short intracellular half-life, so might act as a signal peptide for protein degradation.{{cite journal |vauthors=Rogers S, Wells R, Rechsteiner M |title=Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis |journal=Science |volume=234 |issue=4774 |pages=364–8 |year=1986 |pmid=2876518 |doi=10.1126/science.2876518|bibcode=1986Sci...234..364R }} This may be mediated via the proteasome{{cite journal |vauthors=Reverte CG, Ahearn MD, Hake LE |title=CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome |journal=Dev. Biol. |volume=231 |issue=2 |pages=447–58 |year=2001 |pmid=11237472 |doi=10.1006/dbio.2001.0153|doi-access=free }}{{cite journal |vauthors=Spencer ML, Theodosiou M, Noonan DJ |title=NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif |journal=J. Biol. Chem. |volume=279 |issue=35 |pages=37069–78 |year=2004 |pmid=15229225 |doi=10.1074/jbc.M402507200|doi-access=free }} or calpain.{{cite journal |vauthors=Shumway SD, Maki M, Miyamoto S |title=The PEST Domain of IκBα is necessary and sufficient for in vitro degradation by mu-calpain |journal=J. Biol. Chem. |volume=274 |issue=43 |pages=30874–81 |year=1999 |pmid=10521480 |doi=10.1074/jbc.274.43.30874|doi-access=free }}