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PRIM2

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

DNA primase large subunit is an enzyme that in humans is encoded by the PRIM2 gene.{{cite journal | vauthors = Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H| title = Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12 | journal = Genomics | volume = 28 | issue = 2 | pages = 350–3 |date=Feb 1996 | pmid = 8530050 | doi =10.1006/geno.1995.1155 |display-authors=etal}}

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit.{{cite web | title = Entrez Gene: PRIM2A primase, polypeptide 2A, 58kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5558}}

References

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Further reading

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  • {{cite journal |vauthors=Stadlbauer F, Brueckner A, Rehfuess C, etal |title=DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. |journal=Eur. J. Biochem. |volume=222 |issue= 3 |pages= 781–93 |year= 1994 |pmid= 8026492 |doi=10.1111/j.1432-1033.1994.tb18925.x |doi-access=free }}
  • {{cite journal | vauthors=Maruyama K, Sugano S |title=Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. |journal=Gene |volume=138 |issue= 1–2 |pages= 171–4 |year= 1994 |pmid= 8125298 |doi=10.1016/0378-1119(94)90802-8 }}
  • {{cite journal |vauthors=Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal |title=Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library |journal=Gene |volume=200 |issue= 1–2 |pages= 149–56 |year= 1997 |pmid= 9373149 |doi=10.1016/S0378-1119(97)00411-3 }}
  • {{cite journal |vauthors=Coll JM, Hickey RJ, Cronkey EA, etal |title=Mapping specific protein-protein interactions within the core component of the breast cell DNA synthesome |journal=Oncol. Res. |volume=9 |issue= 11–12 |pages= 629–39 |year= 1998 |pmid= 9563011 }}
  • {{cite journal |vauthors=Schneider A, Smith RW, Kautz AR, etal |title=Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit |journal=J. Biol. Chem. |volume=273 |issue= 34 |pages= 21608–15 |year= 1998 |pmid= 9705292 |doi=10.1074/jbc.273.34.21608 |doi-access=free }}
  • {{cite journal | vauthors=Arezi B, Kirk BW, Copeland WC, Kuchta RD |title=Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit |journal=Biochemistry |volume=38 |issue= 39 |pages= 12899–907 |year= 1999 |pmid= 10504261 |doi=10.1021/bi9908991 }}
  • {{cite journal | vauthors=Smith RW, Nasheuer HP |title=Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180 |journal=FEBS Lett. |volume=527 |issue= 1–3 |pages= 143–6 |year= 2002 |pmid= 12220650 |doi=10.1016/S0014-5793(02)03197-6 |s2cid=27852377 }}
  • {{cite journal |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |bibcode=2002PNAS...9916899M |doi-access=free }}
  • {{cite journal |vauthors=Mungall AJ, Palmer SA, Sims SK, etal |title=The DNA sequence and analysis of human chromosome 6 |journal=Nature |volume=425 |issue= 6960 |pages= 805–11 |year= 2003 |pmid= 14574404 |doi= 10.1038/nature02055 |bibcode=2003Natur.425..805M |doi-access= free }}
  • {{cite journal |vauthors=Gerhard DS, Wagner L, Feingold EA, etal |title=The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 }}
  • {{cite journal |vauthors=Rush J, Moritz A, Lee KA, etal |title=Immunoaffinity profiling of tyrosine phosphorylation in cancer cells |journal=Nat. Biotechnol. |volume=23 |issue= 1 |pages= 94–101 |year= 2005 |pmid= 15592455 |doi= 10.1038/nbt1046 |s2cid=7200157 }}

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External links

  • [https://www.ebi.ac.uk/pdbe/pdbe-kb/proteins/P49643 PDBe-KB] provides an overview of all the structure information available in the PDB for Human DNA primase large subunit (PRIM2)

{{DNA replication}}

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