Peanut agglutinin
File:Peanut Agglutinin complexed with Gal-a-1,3-Gal. PDB entry 2dvd.png entry 2dvd]]
Peanut agglutinin (PNA) is plant lectin protein derived from the fruits of Arachis hypogaea. Peanut agglutinin may also be referred to as Arachis hypogaea lectin. Lectins recognise and bind particular sugar sequences in carbohydrates; peanut agglutinin binds the carbohydrate sequence Gal-β(1-3)-GalNAc. The name "peanut agglutinin" originates from its ability to stick together (agglutinate) cells, such as neuraminidase-treated erythrocytes,{{cite web|access-date=2010-03-14|url=http://www.medicago.se/sites/default/files/pdf/productsheets/Arachis_Hypogaea_PNA_v._01.pdf|title=PNA specification sheet Medicago AB}}{{dead link|date=March 2018 |bot=InternetArchiveBot |fix-attempted=yes }} which have glycoproteins or glycolipids on their surface which include the Gal-β(1-3)-GalNAc carbohydrate sequence.
Structure
{{Infobox protein family
| Symbol = Lectin_legB
| Name = Legume lectin domain
| image = PDB 1lem EBI.jpg
| width =
| caption = Structure of the monosaccharide binding site of lentil lectin.{{cite journal |vauthors=Loris R, Casset F, Bouckaert J, etal |title=The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study |journal=Glycoconj. J. |volume=11 |issue=6 |pages=507–17 |date=December 1994 |pmid=7696853 |doi= 10.1007/bf00731301|s2cid=20037257 }}
| Pfam = PF00139
| Pfam_clan = CL0004
| InterPro = IPR001220
| SMART =
| PROSITE = PDOC00278
| MEROPS =
| SCOP = 1lem
| TCDB =
| OPM family =
| OPM protein =
| PDB = }}
The protein is 273 amino acids in length with the first 23 residues acting as a signal peptide which is subsequently cleaved. It has a Uniprot accession of [https://www.uniprot.org/uniprot/P02872 P02872]. There are over 20 structures of this protein in the PDB which reveal and all beta-sheet protein with a tetrameric quaternary structure. It is a member of the Lectin_legB PFAM family.
[http://www.ebi.ac.uk/pdbe/searchResults.html?display=both&term=P02872 Available Structures of peanut agglutinin]
Uses in cell biology and biochemistry
Because peanut agglutinin specifically binds a particular carbohydrate sequence it finds use in a range of methods for cell biology and biochemistry. For example in PNA-affinity chromatography the binding specificity of peanut agglutinin is used to isolate glycosylated molecules which have the sugar sequence Gal-β(1-3)-GalNAc. Peanut agglutinin activity is inhibited by lactose and galactose which compete for the binding site.
Other uses include:
- Potent anti-T cell activity.
- Distinguishing between human lymphocyte subsets.
- Identification of cone cell inner and outer segments and to a lesser extent rod cell inner segments in the mammalian retina.{{cite journal| author=Blanks JC, Johnson LV|title=Specific binding of peanut lectin to a class of retinal photoreceptor cells. A species comparison.|journal=Invest Ophthalmol Vis Sci|year=1984|volume=25|issue=5|pages=546–57|pmid=6715128}}
- Tumour tissue determination for transitional mucosa malignancies.
- Identification of mammalian-infective metacyclic promastigote Leishmania major parasites from other life cycle forms also found in the sandfly host.
- Identification of the outer acrosome membrane in sperm, indicating acrosome integrity. {{cite journal|last1=Blumer|first1=Camile Garcia|last2=Restelli|first2=Adriana Ester|last3=Giudice|first3=Paula Toni Del|last4=Soler|first4=Thiesa Butterby|last5=Fraietta|first5=Renato|last6=Nichi|first6=Marcilio|last7=Bertolla|first7=Ricardo Pimenta|last8=Cedenho|first8=Agnaldo Pereira|title=Effect of varicocele on sperm function and semen oxidative stress|journal=BJU International|volume=109|issue=2|pages=259–265|doi=10.1111/j.1464-410X.2011.10240.x|pmid=21592296|year=2012|s2cid=23548329 }}
See also
References
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