Phikmvvirus

{{Virusbox

| taxon = Phikmvvirus

}}

Phikmvvirus is a genus of viruses that infect bacteria. There are currently 16 species in this genus including the type species Pseudomonas virus phiKMV.{{cite web|title=Viral Zone|url=http://viralzone.expasy.org/all_by_species/710.html|publisher=ExPASy|accessdate=15 June 2015}}{{cite web|url=https://ictv.global/taxonomy|title=Virus Taxonomy: 2019 Release|last=|first=|date=|website=talk.ictvonline.org|publisher=International Committee on Taxonomy of Viruses|access-date=4 May 2020|quote=}}

Bacteriophage phiKMV{{cite journal |last1=Lavigne |first1=Rob |last2=Burkal'tseva |first2=Maria V. |last3=Robben |first3=Johan |last4=Sykilinda |first4=Nina N. |last5=Kurochkina |first5=Lidia P. |last6=Grymonprez |first6=Barbara |last7=Jonckx |first7=Bart |last8=Krylov |first8=Victor N. |last9=Mesyanzhinov |first9=Vadim V. |last10=Volckaert |first10=Guido |title=The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa |journal=Virology |volume=312 |issue=1 |pages=49–59 |year=2003 |pmid=12890620 |doi=10.1016/S0042-6822(03)00123-5|display-authors=8 |doi-access=free }} and its relatives are known to be highly virulent phages, producing large ({{Convert|3|-|15|mm|abbr=on}} diameter) clear plaques on a susceptible host.{{cite journal |last1=Ceyssens |first1=P.-J. |last2=Lavigne |first2=R. |last3=Mattheus |first3=W. |last4=Chibeu |first4=A. |last5=Hertveldt |first5=K. |last6=Mast |first6=J. |last7=Robben |first7=J. |last8=Volckaert |first8=G. |title=Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the KMV Subgroup within the T7 Supergroup |journal=Journal of Bacteriology |volume=188 |issue=19 |pages=6924–31 |year=2006 |pmid=16980495 |pmc=1595506 |doi=10.1128/JB.00831-06}}{{cite journal |last1=Lammens |first1=E. |last2= Ceyssens |first2=P.-J. |last3=Voet |first3=M. |last4=Hertveldt |first4=K. |last5= Lavigne |first5=R. | last6=Volckaert |first6=G. |title= Representational Difference Analysis (RDA) of bacteriophage genomes |journal= Journal of Microbiological Methods |volume=77 |issue=2 |pages=207–13 |year=2009|pmid=19232531 |doi=10.1016/j.mimet.2009.02.006}} The only reported exception is phage LKA1, which yields small plaques ({{Convert|1|mm|abbr=on}}) surrounded by a halo. While all other P. aeruginosa-specific phikmvviruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysaccharide layer.

Taxonomy

The following species are recognized:

Virology

Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately {{Convert|60|nm|abbr=on}} and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the genus Phikmvvirus is classified within the Autographiviridae.{{cite journal |last1=Lavigne |first1=Rob |last2=Seto |first2=Donald |last3=Mahadevan |first3=Padmanabhan |last4=Ackermann |first4=Hans-W. |last5=Kropinski |first5=Andrew M. |title=Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools |journal=Research in Microbiology |volume=159 |issue=5 |pages=406–14 |year=2008 |pmid=18555669 |doi=10.1016/j.resmic.2008.03.005|doi-access=free }}

Genus	Structure \|\| Symmetry	Capsid	Genomic arrangement	Genomic segmentation
class="wikitable sortable" style="text-align:center"
Phikmvvirus	Head-Tail	T=7	Non-enveloped	Linear	Monopartite

Life cycle

Viral replication is cytoplasmic. Entry into the host cell is achieved by absorption into the host cell. DNA-templated transcription is the method of transcription. The virus exits the host cell by lysis, and holin/endolysin/spanin proteins. Bacteria serve as the natural host. Transmission routes are passive diffusion.

Genus	Host details	Tissue tropism	Entry details	Release details	Replication site	Assembly site	Transmission
class="wikitable sortable" style="text-align:center"
Phikmvvirus	Bacteria	None	Injection	Lysis	Cytoplasm	Cytoplasm	Passive diffusion

References

External links

[http://viralzone.expasy.org/all_by_species/710.html Viralzone: Phikmvlikevirus]
[http://ictvonline.org/virusTaxonomy.asp ICTV]

Category:Caudovirales

Category:Virus genera