Pierce Protein Assay
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The Pierce Protein Assay is a method of protein quantification. It provides quick estimation of the protein amount in a given sample.{{Cite web|url = https://tools.lifetechnologies.com/content/sfs/brochures/1602063-Protein-Assay-Handbook.pdf|title = Protein Assay Handbook|date = |accessdate = 9 April 2015|website = |publisher = Life technologies|last = |first = }}
Protocol
The assay is separated into three main parts:
preparation of the Diluted Albumin (BSA) Standards,
preparation of the bicinchoninic acid (BCA) working reagent,
and quantification of proteins (using either test tube or microplate procedure).
Advantages and disadvantages
= Advantages =
This method is able to detect as low as 25 μg/ml and up to 2000 μg/ml of protein in a 65 ul sample, using standard protocol.
This method may be preferred for samples containing detergents or other reducing agents.
This method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford) Assays.
This method has a stable end point.
= Disadvantages =
This method has greater protein-to-protein variability than the BCA Assay.