Premnaspirodiene oxygenase

{{Short description|Class of enzymes}}

{{Infobox enzyme

| Name = Premnaspirodiene oxygenase

| EC_number = 1.14.13.121

| CAS_number =

| GO_code =

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Premnaspirodiene oxygenase ({{EC number|1.14.13.121}}, HPO, Hyoscymus muticus premnaspirodiene oxygenase) is an enzyme with systematic name (-)-vetispiradiene,NADPH:oxygen 2alpha-oxidoreductase.{{cite journal | vauthors = Takahashi S, Yeo YS, Zhao Y, O'Maille PE, Greenhagen BT, Noel JP, Coates RM, Chappell J | title = Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates | journal = The Journal of Biological Chemistry | volume = 282 | issue = 43 | pages = 31744–54 | date = October 2007 | pmid = 17715131 | pmc = 2695360 | doi = 10.1074/jbc.M703378200 | doi-access = free }} This enzyme catalyses the following chemical reaction

: (-)-vetispiradiene + 2 NADPH + 2 H⁺ + 2 O₂ $\backslash rightleftharpoons$ solavetivone + 2 NADP⁺ + 3 H₂O (overall reaction)

:(1a) (-)-vetispiradiene + NADPH + H⁺ + O₂ $\backslash rightleftharpoons$ solavetivol + NADP⁺ + H₂O

:(1b) solavetivol + NADPH + H⁺ + O₂ $\backslash rightleftharpoons$ solavetivone + NADP⁺ + 2 H₂O

Premnaspirodiene oxygenase heme-thiolate protein (P-450).