Pseudomonas sRNA P16

{{DISPLAYTITLE:Pseudomonas sRNA P16}}

{{Infobox rfam

| Name = Pseudomonas sRNA P16

| image = RF00628.jpg

| width =

| caption = Predicted secondary structure and sequence conservation of P16

| Symbol = P16

| AltSymbols =

| Rfam = RF00628

| miRBase =

| miRBase_family =

| RNA_type = Gene

| Tax_domain = Bacteria

| GO =

| SO = {{SO|0000655}}

| CAS_number =

| EntrezGene =

| HGNCid =

| OMIM =

| PDB =

| RefSeq =

| Chromosome =

| Arm =

| Band =

| LocusSupplementaryData =

}}

Pseudomonas sRNA P16 is a ncRNA that was predicted using bioinformatic tools in the genome of the opportunistic pathogen Pseudomonas aeruginosa and its expression verified by northern blot analysis.{{cite journal |vauthors=Livny J, Brencic A, Lory S, Waldor MK |title=Identification of 17 Pseudomonas aeruginosa sRNAs and prediction of sRNA-encoding genes in 10 diverse pathogens using the bioinformatic tool sRNAPredict2 |journal=Nucleic Acids Res. |volume=34 |issue=12 |pages=3484–3493 |year=2006 |pmid=16870723 |doi=10.1093/nar/gkl453 |pmc=1524904}} P16 sRNA appears to be conserved across several Pseudomonas species and is consistently located downstream of a predicted TatD deoxyribonuclease gene. P16 has a predicted Rho independent terminator at the 3′ end but the function of P16 is unknown.

It has been shown that this sRNA is transcribed from an RpoS-dependent promoter under positive, probably indirect GacA control in two Pseudomonas species. It was renamed RgsA (for regulation by GacA and stress).{{Cite journal|last1=González|first1=Nicolas|last2=Heeb|first2=Stephan|last3=Valverde|first3=Claudio|last4=Kay|first4=Elisabeth|last5=Reimmann|first5=Cornelia|last6=Junier|first6=Thomas|last7=Haas|first7=Dieter|date=2008-04-13|title=Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species|journal=BMC Genomics|volume=9|pages=167|doi=10.1186/1471-2164-9-167|issn=1471-2164|pmc=2375449|pmid=18405392 |doi-access=free }} RpoS mRNA expression is repressed by RgsA during the exponential phase. The Hfq RNA chaperone is required for the repression.{{Cite journal|last1=Lu|first1=Pei|last2=Wang|first2=Yifei|last3=Hu|first3=Yangbo|last4=Chen|first4=Shiyun|date=April 2018|title=RgsA, an RpoS-dependent sRNA, negatively regulates rpoS expression in Pseudomonas aeruginosa|journal=Microbiology|volume=164|issue=4|pages=716–724|doi=10.1099/mic.0.000632|issn=1465-2080|pmid=29473822|doi-access=free}}