Pseudomonas virus gh1

{{short description|Species of virus}}

{{Virusbox

| name = Bacteriophage gh-1

| image = TEM gh1.png

| image_caption = Electron micrograph of bacteriophage gh-1 negatively stained with uranyl acetate. Magnification X 300,000. Two fibers attached to the wedge-shaped tail are visible on the bacteriophage, as indicated by an arrow.

| parent = Ghunavirus

| species = Ghunavirus gh1

}}

Bacteriophage gh-1 is a bacteriophage capable of infecting susceptible strains of Pseudomonas putida.{{cite journal

| last1 = Lee

| first1 = L.

| last2 = Boezi

| first2 = J.

| title = Characterization of bacteriophage gh-1 for Pseudomonas putida.

| journal = Journal of Bacteriology

| volume = 92

| issue = 6

| pages = 1821–1827

|date= 1966

| publisher = American Society for Microbiology

| doi = 10.1128/JB.92.6.1821-1827.1966

| pmid = 5958111

| pmc = 316266

| language = English

}} It is a member of the genus Ghunavirus, family Autotranscriptaviridae, order Autographivirales.{{cite web|title=History of the taxon: Species: Ghunavirus gh1 (2024 Release, MSL #40)|url=https://ictv.global/taxonomy/taxondetails?taxnode_id=202400585&taxon_name=Ghunavirus%20gh1|publisher=International Committee on Taxonomy of Viruses|access-date=5 April 2025}} It was first isolated in 1966 from a sample taken from the aeration tank at a sewage plant in East Lansing, Michigan.

Sedimentation analysis indicates that gh-1 carries its genetic payload in the form of a 37,359 bp linear strand of dsDNA,{{cite journal

| last1 = Lee

| first1 = L.

| last2 = Boezi

| first2 = J.

| title = Sedimentation Analysis of Pseudomonas putida A.3.12 Bacteriophage gh-1 Deoxyribonucleic Acid

| journal = Journal of Virology

| volume = 1

| issue = 6

| pages = 1274–1276

|date=1967

| publisher = American Society for Microbiology

| doi = 10.1128/JVI.1.6.1274-1276.1967

| language = English

| pmid = 5621492

| pmc = 375419

}} inside an icosahedronal capsid 50 nm in diameter.

One-step growth experiments indicate that the latent period is approximately 21 min, with a burst size of 103.

It has been shown that this phage group requires an intact O-antigen on its host's outer membrane in order to successfully replicate and it is thus likely that lipopolysaccharide acts as the phage receptor.{{cite journal

| last1 = Kovalyova

| first1 = I.

| last2 = Kropinski

| first2 = A.

| title = The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida - Evidence for close relationship to the T7 group

| journal = Journal of Virology

| volume = 311

| issue = 2

| pages = 305–315

|date=2003

| doi = 10.1016/S0042-6822(03)00124-7

| pmid = 12842620

| language = English

| doi-access = free

}}