RNA-induced transcriptional silencing
{{short description|Form of RNA interference}}
{{redirect|RITS|the juvenile prison Rhode Island Training School|Rhode Island Department of Children, Youth & Families}}
RNA-induced transcriptional silencing (RITS) is a form of RNA interference by which short RNA molecules – such as small interfering RNA (siRNA) – trigger the downregulation of transcription of a particular gene or genomic region. This is usually accomplished by posttranslational modification of histone tails (e.g. methylation of lysine 9 of histone H3) which target the genomic region for heterochromatin formation. The protein complex that binds to siRNAs and interacts with the methylated lysine 9 residue of histones H3 (H3K9me2) is the RITS complex.
RITS was discovered in the fission yeast Schizosaccharomyces pombe, and has been shown to be involved in the initiation and spreading of heterochromatin in the mating-type region and in centromere formation. The RITS complex in S. pombe contains at least a piwi domain-containing RNase H-like argonaute, a chromodomain protein Chp1, and an argonaute interacting protein Tas3 which can also bind to Chp1,{{cite journal |vauthors=Verdel A, Jia S, Gerber S, Sugiyama T, Gygi S, Grewal S, Moazed D |title=RNAi-mediated targeting of heterochromatin by the RITS complex |journal=Science |volume=303 |issue=5658 |pages=672–6 |year=2004 |pmid=14704433 |doi=10.1126/science.1093686 |pmc=3244756|bibcode=2004Sci...303..672V }} while heterochromatin formation has been shown to require at least argonaute and an RNA-dependent RNA polymerase.{{cite journal |vauthors=Irvine D, Zaratiegui M, Tolia N, Goto D, Chitwood D, Vaughn M, Joshua-Tor L, Martienssen R |title=Argonaute slicing is required for heterochromatic silencing and spreading |journal=Science |volume=313 |issue=5790 |pages=1134–7 |year=2006 |pmid=16931764 |doi=10.1126/science.1128813|bibcode=2006Sci...313.1134I |s2cid=42997104 }} Loss of these genes in S. pombe results in abnormal heterochromatin organization and impairment of centromere function,{{cite journal |vauthors=Volpe T, Kidner C, Hall I, Teng G, Grewal S, Martienssen R |title=Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi |journal=Science |volume=297 |issue=5588 |pages=1833–7 |year=2002 |pmid=12193640 |doi=10.1126/science.1074973|bibcode=2002Sci...297.1833V |s2cid=2613813 |doi-access=free }} resulting in lagging chromosomes on anaphase during cell division.{{cite journal |vauthors=Volpe T, Schramke V, Hamilton G, White S, Teng G, Martienssen R, Allshire R |title=RNA interference is required for normal centromere function in fission yeast |journal=Chromosome Res |volume=11 |issue=2 |pages=137–46 |year=2003 |pmid=12733640 |doi=10.1023/A:1022815931524|s2cid=23813417 }}
Function and mechanisms
The maintenance of heterochromatin regions by RITS complexes has been described as a self-reinforcing feedback loop, in which RITS complexes stably bind the methylated histones of a heterochromatin region using the Chp1 protein and induce co-transcriptional degradation of any nascent messenger RNA (mRNA) transcripts, which are then used as RNA-dependent RNA polymerase substrates to replenish the complement of siRNA molecules to form more RITS complexes.{{cite journal |vauthors=Sugiyama T, Cam H, Verdel A, Moazed D, Grewal S |title=RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production |journal=Proc Natl Acad Sci USA |volume=102 |issue=1 |pages=152–7 |year=2005 |pmid=15615848 |doi=10.1073/pnas.0407641102 |pmc=544066|doi-access=free |bibcode=2005PNAS..102..152S }} The RITS complex localizes to heterochromatic regions through the base pairing of the nascent heterochromatic transcripts as well as through the Chp chromodomain which recognizes methylated histones found in heterochromatin.{{Cite journal|last1=Volpe|first1=Tom|last2=Martienssen|first2=Robert A.|date=2011-09-01|title=RNA Interference and Heterochromatin Assembly|journal=Cold Spring Harbor Perspectives in Biology|volume=3|issue=9|pages=a003731|doi=10.1101/cshperspect.a003731|issn=1943-0264|pmc=3181039|pmid=21441597}} Once incorporated into the heterochromatin, the RITS complex is also known to play a role in the recruitment of other RNAi complexes as well as other chromatin modifying enzymes to specific genomic regions.{{Cite journal|last=Moazed|first=Danesh|date=2009-01-22|title=Small RNAs in transcriptional gene silencing and genome defence|journal=Nature|volume=457|issue=7228|pages=413–420|doi=10.1038/nature07756|issn=0028-0836|pmc=3246369|pmid=19158787|bibcode=2009Natur.457..413M}} Heterochromatin formation, but possibly not maintenance, is dependent on the ribonuclease protein dicer, which is used to generate the initial complement of siRNAs.{{cite journal |vauthors=Noma K, Sugiyama T, Cam H, Verdel A, Zofall M, Jia S, Moazed D, Grewal S |title=RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing |journal=Nat Genet |volume=36 |issue=11 |pages=1174–80 |year=2004 |pmid=15475954 |doi=10.1038/ng1452|doi-access=free }}
Importance in other species
The relevance of observations from fission yeast mating-type regions and centromeres to mammals is not clear, as some evidence suggests that heterochromatin maintenance in mammalian cells is independent of the components of the RNAi pathway.{{cite journal |vauthors=Wang F, Koyama N, Nishida H, Haraguchi T, Reith W, Tsukamoto T |title=The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells |journal=Mol Cell Biol |volume=26 |issue=11 |pages=4028–40 |year=2006 |pmid=16705157 |doi=10.1128/MCB.02189-05 |pmc=1489094}} It is known, however, that plants and animals have analogous mechanism for small RNA-guided heterochromatin formation, and it is believed that the mechanisms described above for S. pombe are highly conserved and play some role in heterochromatin formation in mammals as well. In higher eukaryotes, RNAi-dependent heterochromatic silencing appears to play a larger role in germline cells than in primary cells or cell lines, and is only one of the many different forms of gene silencing used throughout the genome, making it more difficult to study.{{Cite journal|last1=Volpe|first1=Tom|last2=Martienssen|first2=Robert A.|date=2011-09-01|title=RNA Interference and Heterochromatin Assembly|journal=Cold Spring Harbor Perspectives in Biology|volume=3|issue=9|pages=a003731|doi=10.1101/cshperspect.a003731|issn=1943-0264|pmc=3181039|pmid=21441597}}
The role of RNAi in transcriptional gene silencing in plants has been characterized fairly well, and functions primarily through DNA methylation via the RdDM pathway. In this process, which is distinct from the process described above, argonaut-bound siRNA recognizes nascent RNA transcripts or the target DNA to guide the methylation and silencing of the target genomic region.{{Cite journal|last1=Matzke|first1=Marjori|last2=Kanno|first2=Tatsuo|last3=Daxinger|first3=Lucia|last4=Huettel|first4=Bruno|last5=Matzke|first5=Antonius J. M.|date=2009-06-01|title=RNA-mediated chromatin-based silencing in plants|journal=Current Opinion in Cell Biology|volume=21|issue=3|pages=367–376|doi=10.1016/j.ceb.2009.01.025|issn=1879-0410|pmid=19243928}}